L'intérêt de la spectrométrie de masse à très haut champ (FT-ICR) pour la métabolomique

National audienc

Caractérisation rapide du xénométabolome par spectrométrie de masse à très haute résolution combinée à un filtre de défaut de masse

Caractérisation rapide du xénométabolome par spectrométrie de masse à très haute résolution combinée à un filtre de défaut de masse. 7. Journées Scientifiques du Réseau Français de Métabolomique et Fluxomiqu

High throughput metabolomics by ion mobility mass spectrometry

High throughput metabolomics by ion mobility mass spectrometry. 65. ASMS Conference on Mass Spectrometry and Allied Topic

Different ion mobility‐mass spectrometry coupling techniques to promote metabolomics

International audienceMetabolomics has become increasingly popular in recent years for many applications ranging from clinical diagnosis, human health to biotechnological questioning. Despite technological advances, metabolomic studies are still currently limited by the difficulty of identifying all metabolites, a class of compounds with great chemical diversity. Although lengthy chromatographic analyses are often used to obtain comprehensive data, many isobar and isomer metabolites still remain unresolved, which is a critical point for the compound identification. Currently, ion mobility spectrometry is being explored in metabolomics as a way to improve metabolome coverage, analysis throughput and isomer separation. In this review, all the steps of a typical workflow for untargeted metabolomics are discussed considering the use of an ion mobility instrument. An overview of metabolomics is first presented followed by a brief description of ion mobility instrumentation. The ion mobility potential for complex mixture analysis is discussed regarding its coupling with a mass spectrometer alone, providing gas-phase separation before mass analysis as well as its combination with different separation platforms (conventional hyphenation but also multidimensional ion mobility couplings), offering multidimensional separation. Various instrumental and analytical conditions for improving the ion mobility separation are also described. Finally, data mining, including software packages and visualization approaches, as well as the construction of ion mobility databases for the metabolite identification are examined

How to really perform high throughput metabolomic analyses efficiently?

International audienceHigh-throughput analyses are based on technologies characterized by their rapidity, simplicity, sensitivity, robustness, low cost and high efficiency. They offer the potential for screening a large number of samples per day, which cannot be done using classical methods. High-throughput analyses have shown their feasibility and efficiency in multidisciplinary fields such as drug screening, bioassays of compound against mycobacteria. Another successful application is based on direct introduction mass spectrometry for the analysis of very complex organic materials in petroleomics. High-throughput analyses appear to be very attractive in metabolomics, which aims to study the interface between the chemical universe and biology, to detect general metabolic disruptions induced by external factors through a metabolite profiling approach. In this review, we focus on high-throughput metabolomics using high resolution mass spectrometry (HRMS). Specifically, performances and limits of available analytical techniques dedicated to high-throughput analyses, including sample introduction methods, HRMS and data processing tools are discussed

Identification of xenobiotic metabolites from biological fluids using flow injection analysis high‐resolution mass spectrometry and post‐acquisition data filtering: Identification of xenometabolites using FIA‐HRMS and data filtering

International audienceno abstrac

High throughput metabolomics based on ultra high resolution detection and dedicated chemometric tools as an efficient approach to address health monitoring

High throughput metabolomics based on ultra high resolution detection and dedicated chemometric tools as an efficient approach to address health monitoring. 23. International Scientific Conference of LAA