Hematological profile of sickle cell disease in Chhattisgarh

Background: Sickle cell disease hemoglobinopathy gets inherited in autosomal recessive pattern. In sickle cell disease substitution of amino acid valine for glutamic acid at the sixth position on beta globin chain takes place resulting in sickled hemoglobin which is a hemoglobin tetramer.Methods: A prospective observational study was conducted in the Sickle Cell Institute, Raipur, India, and Department of Pharmacology in collaboration with Department of Biochemistry, Pt. J.N.M. Medical College, Raipur, Chhattisgarh, India, from February 2018 to June 2018. Patients included were in the steady state for a long period of time without any symptoms related to sickle cell disease or any other diseases which could affect hematological parameters. Subjects transfused in the last three months were excluded. Student t test and Pearson Correlation Coefficient test was done on stat pages and socscistatistics calculators. P-value<0.05 was considered as statistically significant.Results: A total of 50 subjects of sickle cell disease homozygous (SS) were studied for hematological parameters. The mean age±SD of 50 subjects were 13.3±9.24 years. Out of 50 subjects, 35 were males and 15 were females. Total RBC count, mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) was low in present study. Significant inverse correlation was found in females between HbA2 and HbF, p=0.01, while it was insignificant and negatively correlated in males being 0.23.Conclusions: Sickle cell disease homozygous is a common and challenging health problem of Chhattisgarh population

Effects of yoga in modifying blood pressure in patients of type 2 diabetes mellitus

Background: The incidence of diabetes mellitus is increasing with the increase in unhealthy dietary habits, physical inactivity and sedentary lifestyle. Hypertension complicated with diabetes further aggravates the problem. Yoga has been found to be helpful in delaying the progression and complications of the disease. The present study was undertaken with an aim to evaluate the effect of yoga in modifying blood pressure in patients of diabetes mellitus.Methods: Thirty patients of diabetes mellitus who were known case of hypertension were taken and their fasting and post-prandial blood glucose levels, systolic and diastolic blood pressure and waist circumference were analysed before and after ninety days of yoga in the department of physiology and biochemistry.Results: Systolic and diastolic blood pressure, waist circumference and fasting and post-prandial blood glucose level showed significant reduction (p value <0.05).Conclusions: This study emphasizes the importance of yoga in the control and management of type 2 diabetes mellitus and hypertension

Facile fabrication of Nishamalaki churna mediated silver nanoparticles with antibacterial application

Antimicrobial resistance (AMR) is one of the most serious threats to today's healthcare system. The prime factor behind increasing AMR is the formation of complex bacterial biofilms which acts as the protective shield between the bacterial cell and the antimicrobial drugs. Among various nanoformulations, green synthesized metallic silver nanoparticles are currently gaining research focus in safely breaking bacterial biofilms due to the inherent antimicrobial property of silver. In the current work, the aqueous extract of the ayurvedic formulation Nishamalaki churna is used to exhibit one pot green synthesis of silver nanoparticles. The physicochemical characteristics of Nishamalaki churna extract mediated AgNPs were evaluated using various analytical techniques, like UV–Visible spectrophotometer, FT-IR spectroscopy, SEM, XRD, DLS-Zeta potential analyzer etc. The synthesized spherical AgNPs were well formed within the size range of 30 nm to 80 nm. Furthermore, the synthesized AgNPs showed potent antibacterial effects against two primary AMR-causing bacterial species like Staphylococcus aureus and Pseudomonas aeruginosa with the successful destruction of their biofilm formation. Additionally, these AgNPs have shown profound antioxidant and anti-inflammatory activities as desirable add-on effects required by a prospective antibacterial agent