Firewall interface for java FTP SMTP and HTTP servers

The objective of my thesis is to develop a firewall interface for FTP, HTTP and SMTP servers. A firewall interface is a graphical user interface (GUI) to configure the properties of the server. Using this interface, we can spy a user, block and unblock the IP address of a client, display statistics of user and server. We can track all the user actions while he is connected to the server. These actions are logged and based on the log data we can deny access to users performing illegal operations. All the servers and interfaces are written in Java. Java was chosen because it is platform independent

Identification and characterization of presumptive bovine mammary stem cells

An understanding of the characteristics and regulation of mammary stem cells (MaSCs) is needed to gain insight into normal gland development and carcinogenesis. Previous profiling of MaSCs relied upon immunophenotypic selection of enzymatically dispersed cells by flow cytometry. However, these approaches involved the selection of cells that are removed from their tissue location and cellular microenvironment. In this study, I have utilized an alternative approach called laser microdissection, to excise putative MaSCs, based upon their ability to retain bromodeoxyuridine labeled DNA for an extended period, and control cells from their in situ locations in prepubertal bovine mammary cryosections. First, I established a protocol to immunostain putative MaSCs in tissue cryosections and isolate RNA of high quality. Next, I excised putative MaSCs and control cells from immunostained cryosections using laser microdissection. Global gene expression analysis by microarray provided evidence that MaSCs were located in the basal epithelium and progenitor cells located in suprabasal layers. A number of genes that were up-regulated in MaSCs and progenitor cells were identified and these are potential biomarkers. Analysis of the expression pattern of four genes (NR5A2, NUP153, HNF4A and FNDC3B) by immunohistochemistry showed that the protein expression profile was consistent with microarray data. Detailed immunohistochemical analyses of NR5A2, NUP153, HNF4A and FNDC3B in calf and cows (at various stages of lactation) revealed that their frequency and distribution were consistent with stem/progenitor cell characteristics. Finally, I attempted to manipulate stem/progenitor cells number using cultures of primary mammary epithelial cells. Expansion of stem/progenitor cell is a prerequisite for stem cell therapeutics and facilitates stem cell research. The effect of xanthosine on bovine mammary epithelial cells (MEC) was evaluated. The result of this study showed that xanthosine treatment increased cell proliferation, promoted symmetric cell division and increased expression of telomerase and a novel stem cell marker (FNDC3B). Together, these studies identified novel, potential markers for MaSCs and progenitor cells, and supported the ability of xanthosine to increase stem/progenitor cell number

Comparative evaluation of effect of green tea and green coffee on blood cholesterol level and body weight in albino rats

Background: Overweight and obesity are chronic medical condition responsible for risk factors for many non-communicable diseases. Development of rapid upsurge of these condition in medium and low-income group countries increased the awareness and uses of many types of remedies. Along with other natural herbal products green tea and green coffee have also became popular in communities. Aim of this study was to compare the effect of green tea and green coffee on blood cholesterol and weight in experimental animals.Methods: The experiment of this study was conducted in the Department of Pharmacology, MGM Medical college, Jamshedpur after ethical approval. 24 albino rats were selected and divided randomly in four groups A B C D. Group ‘A’ was kept as control group, group ‘B’ was given fat rich diet to produce hypercholesterolemia, group ‘C’ and ‘D’ were given green tea and green coffee respectively along with cholesterol rich diet. Weight and cholesterol level of rats were measured on 1st day, 2nd week and 4th week.Results: There was significant increase in serum cholesterol in non-treated animals as compared to control group but parameter showed significant less rise of cholesterol and weight in treated group by both green tea and green coffee than non-treated group. The comparative effect of green tea and green coffee had not much difference.Conclusions: This study has provided the information that natural products like green tea and green coffee are useful and helpful in preventive therapy of dyslipidemia and obesity