PENGARUH PERASAN DAUN SURUHAN (Peperomia pellucida) TERHADAP MORTALITAS Aedes aegypti

The research aimed to find out the effect of suruhan (Peperomia pellucida) leaves extract to the mortality of Aedes aegypti. The method applied in this research was laboratory experiments.The research was conducted in Zoology Laboratory of Department of Biology, State University of Gorontalo, from August to September 2019. The research employed Completely Randomized Design (CRD) through 4 treatments namely 0%, 15%, 20%, and 25%, as well as 3 replications. In Addition, every treatment involved 10-second instar larvae of Aedes aegypti as test animal. The ANAVA F-Test analysis was to find out the effect of treatment to mortality of Aedes aegypti and LSD (Least Significant Difference) analysis was to find out difference of every treatment. The mortality of Aedes aegypti was not found at concentration 0%, the mortality of Aedes aegypti with average 2,33 animals were found at concentration 15%, the mortality of Aedes aegypti with average 5,00 animals were found at concentration 20%, and the mortality of Aedes aegypti with average 6,67 animals were found at concentration 25%. In addition, the ANAVA F-Test analysis revealed effect of treatment to mortality of Aedes aegypti (

In Silico Analysis of Phalaenopsis Orchid Homeobox1 (POH1) Functional Gene for Shoot Development in Phalaenopsis Orchid

The most favorite ornamental crop in Indonesia is orchid which benefited as floriculture. Therefore, the quality of this crop must be improved.  Biotechnology is appropriate to be used to improve the quality and quantity of orchid plants. To conduct this method, researchers must know what genes function in plant development. In Phalaenopsis orchids, the gene has been identified as homeobox genes called Phalaenopsis Orchid Homeobox1 (POH1). This research aims to conduct in silico analysis of the gene. The materials were retrieved from mRNA and amino acid databases. Then, the materials are aligned, visualized, motif location analysis, motif function discovery, phylogenetic construction, and protein 3D structural modelling. Based on mRNA and amino acid alignment, there are 4 domain regions that are conserved in POH1 and other homologous genes, such as KNOX1, KNOX2, ELK Domain, and Homeobox KN Domain, which roles as a transcription factor involved in plant development. SWISS-MODEL and ColabFold were used in protein modelling of the protein. By ColabbFold modelling, the modelling prediction uses 325 residues, higher than SWISS-MODEL in 59 residues. ColabFold validation by Ramachandra Plot depicts having the most favourite regions is 68.6%, while SWISS-MODEL is 92.3%. Another validation parameter is overall quality factor and QMEAN Score. Protein modelling by ColabFold has overall quality factor 89.252 and QMEAN Score 0.41 ± 0.05. However, SWISS-MODEL 3D prediction has overall quality factor 98.039 and QMEAN score of 0.71 ± 0.11.