Association of miR-125b expression with the promoter hypermethylation of a panel of tumour suppressor genes.

<p>(A)DAPK1 (B) p16 (C) RASSF1A (D) PTEN (E) BRCA1 (F) p14. Positive and negative depicts the presence or absence of promoter hypermethylation of the specified tumour suppressor gene respectively.</p

Association between miR-125b expression and clinicopathological characteristics in epithelial ovarian cancer.

<p>Association between miR-125b expression and clinicopathological characteristics in epithelial ovarian cancer.</p

Sequence of mature miR-125b primer used.

<p>Sequence of mature miR-125b primer used.</p

AUC for ROC curve corresponding to the diagnostic value of miR-125b in EOC.

<p>AUC for ROC curve corresponding to the diagnostic value of miR-125b in EOC.</p

Area under curve (AUC) of receiver operating characteristic (ROC) for miR-125b corresponding to (A)Tumor grade (B)Metastasis (C)Lymph node status and (D)Survival of EOC patients.

<p>Area under curve (AUC) of receiver operating characteristic (ROC) for miR-125b corresponding to (A)Tumor grade (B)Metastasis (C)Lymph node status and (D)Survival of EOC patients.</p

Association of serum miR-125b and clinicopathological characteristics in epithelial ovarian cancer patients with respect to A)Stage, (B)Lymph node metastasis and (C)Distant metastasis status.

<p>Association of serum miR-125b and clinicopathological characteristics in epithelial ovarian cancer patients with respect to A)Stage, (B)Lymph node metastasis and (C)Distant metastasis status.</p

Kaplan Meier survival curve with respect to (A) fold change (B) mucinous and serous histopathological subtypes.

<p>Kaplan Meier survival curve with respect to (A) fold change (B) mucinous and serous histopathological subtypes.</p

Serum expression of miR-125b.

<p>(A) Dot plot showing the relative expression of miR-125b in patients and controls (B) ROC curve for miR-125b exhibiting its diagnostic potential in epithelial ovarian cancer.</p

Table_1_In vitro inhibition of biofilm and virulence factor production in azole-resistant strains of Candida albicans isolated from diabetic foot by Artemisia vulgaris stabilized tin (IV) oxide nanoparticles.docx

The advent of nanotechnology has been instrumental in the development of new drugs with novel targets. Recently, metallic nanoparticles have emerged as potential candidates to combat the threat of drug-resistant infections. Diabetic foot ulcers (DFUs) are one of the dreadful complications of diabetes mellitus due to the colonization of numerous drug-resistant pathogenic microbes leading to biofilm formation. Biofilms are difficult to treat due to limited penetration and non-specificity of drugs. Therefore, in the current investigation, SnO2 nanoparticles were biosynthesized using Artemisia vulgaris (AvTO-NPs) as a stabilizing agent and were characterized using ultraviolet–visible (UV–vis) spectroscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX). Furthermore, the efficacy of AvTO-NPs against biofilms and virulence factors of drug-resistant Candida albicans strains isolated from DFUs was assessed. AvTO-NPs displayed minimum inhibitory concentrations (MICs) ranging from 1 mg/mL to 2 mg/mL against four strains of C. albicans. AvTO-NPs significantly inhibited biofilm formation by 54.8%–87%, germ tube formation by 72%–90%, cell surface hydrophobicity by 68.2%–82.8%, and exopolysaccharide (EPS) production by 69%–86.3% in the test strains at respective 1/2xMIC. Biosynthesized NPs were effective in disrupting established mature biofilms of test strains significantly. Elevated levels of reactive oxygen species (ROS) generation in the AvTO-NPs-treated C. albicans could be the possible cause of cell death leading to biofilm inhibition. The useful insights of the present study could be exploited in the current line of treatment to mitigate the threat of biofilm-related persistent DFUs and expedite wound healing.</p

Role of non-coding RNA networks in leukemia progression, metastasis and drug resistance

Early-stage detection of leukemia is a critical determinant for successful treatment of the disease and can increase the survival rate of leukemia patients. The factors limiting the current screening approaches to leukemia include low sensitivity and specificity, high costs, and a low participation rate. An approach based on novel and innovative biomarkers with high accuracy from peripheral blood offers a comfortable and appealing alternative to patients, potentially leading to a higher participation rate. Recently, non-coding RNAs due to their involvement in vital oncogenic processes such as differentiation, proliferation, migration, angiogenesis and apoptosis have attracted much attention as potential diagnostic and prognostic biomarkers in leukemia. Emerging lines of evidence have shown that the mutational spectrum and dysregulated expression of non-coding RNA genes are closely associated with the development and progression of various cancers, including leukemia. In this review, we highlight the expression and functional roles of different types of non-coding RNAs in leukemia and discuss their potential clinical applications as diagnostic or prognostic biomarkers and therapeutic targets.Other Information Published in: Molecular Cancer License: http://creativecommons.org/licenses/by/4.0/See article on publisher's website: http://dx.doi.org/10.1186/s12943-020-01175-9</p