Interacting residues obtained from docking.

Wild protein, L1802P, F1782V, D1778N, V1311M and M936V variants with ligands including their binding residues and hydrophobic interactions.</p

List of nsSNP’s predicted by Mu-Pro, I mutant and ConSURF.

List of nsSNP’s predicted by Mu-Pro, I mutant and ConSURF.</p

Structural validation and comparison of <i>SCN9A</i> gene.

Structural validation and comparison of SCN9A gene.</p

Prediction of disease causing SNPs by P-Mutant, Meta SNP, PhD SNP and SNP & GO.

Prediction of disease causing SNPs by P-Mutant, Meta SNP, PhD SNP and SNP & GO.</p

High risk non-synonymous SNPs identified by SIFT, Polyphen, Mutpred2 and SNAP2.

High risk non-synonymous SNPs identified by SIFT, Polyphen, Mutpred2 and SNAP2.</p

Fig 3 -

Prediction of functional consequences of nsSNPs by A) SIFT and B) PolyPhen.</p

Cumulative prediction of possible deleterious nature of nsSNPs.

Cumulative prediction of possible deleterious nature of nsSNPs.</p

Pie chart displaying the prevalence of deleterious missense mutations.

Evaluation of 15 In silico tools reveals the percentage and numerical quantity of deleterious nsSNPs.</p

Entire workflow for nsSNPs screening in the <i>SCN9A</i> gene using computational tools.

Entire workflow for nsSNPs screening in the SCN9A gene using computational tools.</p

Procheck-RAMACHANDRAN plot of the native <i>SCN9A</i> predicted model.

Procheck-RAMACHANDRAN plot of the native SCN9A predicted model.</p