6 research outputs found
Field evaluation of fluorescence polarization assay, and comparison with competitive ELISA for the detection of antibodies against Brucella melitensis in sheep in Sicily, Italy
Ag/ZnO/PMMA nanocomposites for efficient water reuse
This work attempts to produce photocatalytic surfaces for large-scale applications by depositing nanostructured coatings on polymeric substrates. ZnO/poly(methyl methacrylate) (PMMA) composites were prepared by low-temperature atomic layer deposition (ALD) of ZnO on PMMA substrates. In addition, to increase the photocatalytic and antibacterial activities of ZnO films, Ag nanoparticles were added on ZnO surfaces using plasma-enhanced ALD. The morphology, crystallinity, and chemical composition of the specimens were meticulously examined by scanning and transmission electron microscopies, energy-dispersive X-ray spectroscopy, and X-ray diffraction analyses. The noteworthy photocatalytic activity of the nanocomposites was proved by the degradation of the following organic pollutants in aqueous solution: methylene blue, paracetamol, and sodium lauryl sulfate. The antibacterial properties of the samples were tested using Escherichia coli as a model organism. Moreover, the possible toxic effects of the specimens were checked by biological tests. The present results unambiguously indicate the Ag/ZnO/PMMA nanocomposite as a powerful tool for an advanced wastewater treatment technology.peer-reviewe
Putting Regulatory Data to Work at the Service of Public Health: Utilizing Data Collected Under the Clean Water Act
Under the Clean Water Act, the US Environmental Protection Agency (EPA) collects information from states on intended use and impairment of each water body. We explore the feasibility of using these data, collected for regulatory purposes, for public health analyses. Combining EPA impairment data and stream hydrology information we estimated the percent of stream length impaired for any use, recreational use, or drinking water use per county in the US as exposure variables. For health outcomes we abstracted county-level hospitalization rates of gastrointestinal infections, GI (ICD-9CM 001-009 excluding 008.45) and gastrointestinal symptoms, GS (ICD-9CM 558.9, 787) among US adults aged 65 years and older from the Center for Medicare and Medicaid Services (1991–2004). Linear mixed-effects models were used to assess county-level associations between percent impaired waters and hospitalization rates adjusted for population density, a proxy for person-to-person transmission. Contrary to expectation, both GI and GS were negatively associated with any water impairment in adjusted models (GI: −0.052, 95 % CI: −0.077, −0.028; GS: −0.438, 95 % CI: −0.702, −0.174). GI was also negatively associated with recreational water impairment (−0.079, 95 % CI: −0.123, −0.036 after adjustment). Neither outcome was associated with drinking water impairment. Limited state data were reported to the EPA for specific recreational (27 states) and drinking (13 states) water impairment, thus limiting the power of the study. Though limited, this analysis demonstrates the feasibility of utilizing regulatory data for public health analyses
MORPHOLOGICAL AND BIOLOGICAL VARIABILITY OF STEINERNEMA FELTIAE (NEMATODA, STEINERNEMATIDAE) ITALIAN STRAINS
Steinernema feltiae belongs to the feltiae-kraussei-oregonensis group, clade III, and is an ubiquitarian species of entomopathogenic nematode. It is found in all types of soil and in all types of habitat. Species identification in the entomopathogenic nematodes genera Steinernema is a very complex task, given the broad variability of both morphological and biological traits within populations of a single species. To accomplish this, molecular techniques have been adopted which, however, require additional knowledge. Particularly relevant would be the possibility of testing in a reliable way the variability between different populations of the same species, which might represent different strains with different biological properties. During numerous samplings in Italy, several strains of S. feltiae were isolated. In this paper we analyze the intraspecific variability of the main morphometric and biological data of juveniles and males of 50 Italian populations of S. feltiae. The aim of our work was to determine if morphometric and biological analysis were useful to identify characters having significant diagnostic value, allowing to reliably discriminate among strains. Seven characters routinely computed for morphology (5 morphometrics for infective juveniles, spicula and gubernaculum shapes for males) and 2 biological performances (time to achieve adult stage, reproduction and progeny) were considered. The results showed extreme variability from both morphological and biological points of vie