Higher taxa (i.e. Class/Order), lower taxon (i.e. Family/Genus), and individuals per replicate.

<p>Higher taxa (i.e. Class/Order), lower taxon (i.e. Family/Genus), and individuals per replicate.</p

Proportion of specimens successfully amplified for two different sequence length criteria, Partial barcodes (top) and Full barcodes (bottom).

<p>Replicates are represented by different shapes (circles, pluses and triangles). Treatment designations (A–F) are as indicated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051273#pone-0051273-t001" target="_blank">Table 1</a>.</p

Cumulative frequency graphs of sequence length (base pairs) by treatment.

<p>Circles represent the points at which additional sequences are accumulated. Dashed horizontal lines represent the cutoff between partial barcodes (to the left) and full barcodes (to the right).</p

Nested ANOVA results for treatment and family effects on amplification success.

<p>Nested ANOVA results for treatment and family effects on amplification success.</p

Cumulative frequency graphs of sequence length (base pairs) by lower taxa.

<p>Circles represent the points at which additional sequences are accumulated. Dashed horizontal lines represent the cutoff between partial barcodes (to the left) and full barcodes (to the right).</p

Primers, direction, primer sequence and citation (if available) for all sequences from this study.

<p>Primers, direction, primer sequence and citation (if available) for all sequences from this study.</p

Primer pairs used (forward+reverse) and number of sequence reactions each pair was used.

<p>Primer pairs used (forward+reverse) and number of sequence reactions each pair was used.</p

Preservation treatments showing initial and replenishment concentrations of ethanol, days held in field matrix, day at which DNA extraction was performed, and number of replicates per treatment.

<p>Preservation treatments showing initial and replenishment concentrations of ethanol, days held in field matrix, day at which DNA extraction was performed, and number of replicates per treatment.</p