Lipid peroxidation is a nonparenchymal cell event with reperfusion after prolonged liver ischemia

A proposed mechanism for irreversible ischemic liver damage has been peroxidation of membrane phospholipids by free radicals. However, the hepatocyte is laden with enzymes which are antioxidants and, therefore, ought to be relatively resistant to oxidative injury. To test the hypothesis that free radical damage from ischemia and reperfusion of the liver is a nonparenchymal cell process, we studied an in vivo model of ischemia. A point of transition from reversible to irreversible ischemia was defined at ≥60 min of total ischemia by serial measurements of ATP at control, end of ischemia, and end of reperfusion periods (n = 6 each). Nonparenchymal cells were separated out of 10 livers in each ischemic group using a Percoll gradient. Second derivative spectroscopy did not detect conjugated dienes in any hepatocellular fraction, total cellular, mitochondrial, or microsomal, but did in the nonparenchymal cell fractions of livers from the 60- and 90-min ischemia groups. This in vivo study shows that irreversible ischemia in the rat liver is associated with free radical lipid peroxidation, but that the nonparenchymal cells rather than hepatocytes are the focus of this injury. © 1990

A Rare Case of Isolated torsion of Haematosalphinx Presenting as Acute Abdomen During Pregnancy

Isolated fallopian tubal torsion is a rare non obstetric cause for pain abdomen during pregnancy.We report a case of a 34 year old second gravida who presented with history of amennorrhoe of 7 months, colicky pain in the left iliac fossa , with 2 episodes of vomiting. A Trans vaginal scan revealed a left ovarian cyst. An emergency laparotomy was performed for suspected torsion or haemorrhage into an ovarian cyst. Surprisingly ovaries were normal and a congested and necrotic left tubal torsion with a tubal collection (haematosalphinx) was identified and a salpingectomy was then performed. The differential diagnosis is discussed and the literature is reviewed as it is a very rare clinical entity

Successful Pregnancy Outcome In Maternal Crigler Najjar Syndrome Type II.

Estimated incidence of Crigler-Najjar syndrome(CNS) is 1 case per 1,000,000 births(1 million). The overall prevalence of CN syndrome is unknown, with only several hundred people reported to have this disease. It is interestingly very rare to encounter a pregnant adult women with congenital jaundice. Pregnancy in CN type II patients is a diagnostic and a therapeutic challenge because of the high risk of bilirubin encephalopathy with serious neurological damage as life-threatening complications for the fetus. To date 8 pregnancy outcome have been reported from 5 women and we report the6 woman with a successful 9 th pregnancy outcome. We have discussed detail history, presentation and management during pregnancy and care of the new born

Studies on the effect of doxorubicin on MDA, NO2, NO3, Se-GSH peroxidase and SOD levels in albino rat tissues

Doxorubicin, a highly effective anticancer drug, produces cardiotoxicity, which limits its therapeutic potential. The mechanism of this cardiotoxicity has remained elusive. The use of this drug, however,continues to be limited by its dose-related and time interval toxicity. Reactive oxygen species are hypothesized to be a major factor in the toxicity of doxorubicin. The aim of this work was to investigate theeffect of doxorubicin on dose-related and time interval in rat. The study utilized adult albino rats (120 ± 5 g). They were divided into 8 groups of 7 animals each and were kept under standard laboratory conditions.They had free access to commercial pellet diet and water. The room temperature was maintained at 20 ± 5°C. The study measured rat tissue MDA, NO2, NO3, Se-GSH peroxidase and SOD under Dox stress, and the results indicate that MDA and NO generated in Dox treated samples cause neuro, myo, hepato and renal toxicity. Since SOD and Peroxidase are scavenging molecules, increase in their levels in Dox treated samples may be one of mechanism to overcome Dox caused oxidative stress in Dox treated albino rat

ExtSwap: Leveraging Extended Latent Mapper for Generating High Quality Face Swapping

We present a novel face swapping method using the progressively growing structure of a pre-trained StyleGAN. Previous methods use different encoder decoder structures, embedding integration networks to produce high-quality results, but their quality suffers from entangled representation. We disentangle semantics by deriving identity and attribute features separately. By learning to map the concatenated features into the extended latent space, we leverage the state-of-the-art quality and its rich semantic extended latent space. Extensive experiments suggest that the proposed method successfully disentangles identity and attribute features and outperforms many state-of-the-art face swapping methods, both qualitatively and quantitatively