Variation in the ionic composition in convective showers in Pune, India

Rain water samples of equal volume (100 ml) were collected sequentially from 5 convective shower events for a period of 2 years during the summer monsoon seasons of 1988 and 1989 at Pune. A marked decrease in the concentrations of soil-oriented elements Na+, K+, Ca2+ and Mg2+ was observed in the first few samples in all the events followed by a decrease in pH of the subsequent samples. Surprisingly, SO42- also showed similar variation, indicating that soil is contributing some SO42- in rain water. Washout process appears to be an important mechanism for the removal of the major ions from the atmosphere. However, rainout processes seem to be important for the removal of NO3- ions. The study points out that sequential sampling during a single shower appears to give better information about the relationship among H+, Na+, K+, Ca2+, Mg2+, SO42- and NO3- as compared to bulk sampling for longer duration

Characteristics of aerosols at traffic junctions in Pune city

Vehicular emissions are one of the potential sources of air pollution in the urban regions. Pune, one of the rapidly growing cities of India, faces the severe threat from this problem. Observations of total suspended particulates and Aitken nuclei were conducted at 4 traffic junctions in Pune city during summer (May 2000) and winter (Jan-Feb. 2001). Concentrations of TSP crossed the CPCB limit, recommended for commercial zone, at all the traffic junctions and also the number density of Aitken nuclei was very high. However, aerosols were alkaline in nature, in spite of high concentrations of SO4 and NO3, mainly due to the neutralizing effect of Ca, NH4, Na, Mg and K

Testis and Antler Dysgenesis in Sitka Black-Tailed Deer on Kodiak Island, Alaska: Sequela of Environmental Endocrine Disruption?

It had been observed that many male Sitka black-tailed deer (Odocoileus hemionus sitkensis) on Kodiak Island, Alaska, had abnormal antlers, were cryptorchid, and presented no evidence of hypospadias. We sought to better understand the problem and investigated 171 male deer for phenotypic aberrations and 12 for detailed testicular histopathology. For the low-lying Aliulik Peninsula (AP), 61 of 94 deer were bilateral cryptorchids (BCOs); 70% of these had abnormal antlers. Elsewhere on the Kodiak Archipelago, only 5 of 65 deer were BCOs. All 11 abdominal testes examined had no spermatogenesis but contained abnormalities including carcinoma in situ–like cells, possible precursors of seminoma; Sertoli cell, Leydig cell, and stromal cell tumors; carcinoma and adenoma of rete testis; and microlithiasis or calcifications. Cysts also were evident within the excurrent ducts. Two of 10 scrotal testes contained similar abnormalities, although spermatogenesis was ongoing. We cannot rule out that these abnormalities are linked sequelae of a mutation(s) in a founder animal, followed by transmission over many years and causing high prevalence only on the AP. However, based on lesions observed, we hypothesize that it is more likely that this testis–antler dysgenesis resulted from continuing exposure of pregnant females to an estrogenic environmental agent(s), thereby transforming testicular cells, affecting development of primordial antler pedicles, and blocking transabdominal descent of fetal testes. A browse (e.g., kelp) favored by deer in this locale might carry the putative estrogenic agent(s)

Atmospheric aerosol characteristic studies at Pune and Thiruvananthapuram during INDOEX programme - 1998

Total suspended particulates (TSP) and mass size distribution of aerosols were monitored by using a high-volume air sampler and a low-volume 9-stage Anderson sampler, respectively during March-April 1998 at Pune and during 5-9 January 1998 at Thiruvananthapuram. All these samples were extracted for water-and acid-soluble components using the standard extraction method. The water-soluble extracts were analysed for Cl, SO4, NO3, NH4, Na, K, Ca and Mg and acid-soluble extracts for Al, Fe, Mn, Cu, Zn, Pb, Cd, Ni and Sb. The concentration of TSP varied from 67 to 185 μg/m3 with an average of 122 μg/m3 at Pune and from 35 to 73 μg/m3 with an average of 57 μg/m3 at Thiruvananthapuram. The mass size distribution of aerosols showed bimodal distribution having one peak in coarse mode and the other in fine mode at both the locations. The results of the study are presented here

Studies of trace gases and Aitken Nuclei at inland and coastal stations - A part of INDOEX programme

As a part of the Indian Ocean Experiment (INDOEX) programme, ground-based measurements of trace gases (SO2, NO2, NH3 and O3) and Aitken Nuclei (AN) were carried out at Pune during March-April 1998. Also, measurements of surface ozone and AN were made during 5-10 January 1998, the inter comparison campaign of various instruments at Thiruvananthapuram. The mean concentrations of SO2, NO2, NH3 and O3 at Pune were 3.75, 5.81, 3.21 and 57.8 μg/m3 respectively and that of O3 at Thiruvananthapuram was 44 μg/m3. The average concentration of AN was about five times higher at Pune (19,000/cm3) than that at Thiruvananthapuram (4,400/cm3), indicating more pollution at Pune than at Thiruvananthapuram. The diurnal variation of surface ozone showed high concentrations during the day and low concentrations during the night at both the locations. Diurnal variation of AN at Pune showed a daytime maximum and a night-time minimum. Whereas, at Thiruvananthapuram night-time values were higher than the daytime values which can be attributed to the influence of the land and sea breeze

Localization of ABCG5 and ABCG8 proteins in human liver, gall bladder and intestine

BACKGROUND: The molecular mechanisms that regulate the entry of dietary sterols into the body and their removal via hepatobiliary secretion are now beginning to be defined. These processes are specifically disrupted in the rare autosomal recessive disease, Sitosterolemia (MIM 210250). Mutations in either, but not both, of two genes ABCG5 or ABCG8, comprising the STSL locus, are now known to cause this disease and their protein products are proposed to function as heterodimers. Under normal circumstances cholesterol, but not non-cholesterol sterols, is preferentially absorbed from the diet. Additionally, any small amounts of non-cholesterol sterols that are absorbed are rapidly taken up by the liver and preferentially excreted into bile. Based upon the defects in sitosterolemia, ABCG5 and ABCG8 serve specifically to exclude non-cholesterol sterol entry at the intestinal level and are involved in sterol excretion at the hepatobiliary level. METHODS: Here we report the biochemical and immuno-localization of ABCG5 and ABCG8 in human liver, gallbladder and intestine using cell fractionation and immunohistochemical analyses. RESULTS: We raised peptide antibodies against ABCG5 and ABCG8 proteins. Using human liver samples, cell fractionation studies showed both proteins are found in membrane fractions, but they did not co-localize with caveolin-rafts, ER, Golgi or mitochondrial markers. Although their distribution in the sub-fractions was similar, they were not completely contiguous. Immunohistochemical analyses showed that while both proteins were readily detectable in the liver, ABCG5 was found predominately lining canalicular membranes, whereas ABCG8 was found in association with bile duct epithelia. At the cellular level, ABCG5 appeared to be apically expressed, whereas ABCG8 had a more diffuse expression pattern. Both ABCG5 and ABCG8 appeared to localize apically as shown by co-localization with MRP2. The distribution patterns of ABCG5 and ABCG8 in the gallbladder were very similar to each other. In the small intestine both ABCG5 and ABCG8 appear to line the brush border. However, at the level of the enterocyte, the cellular distribution patterns of ABCG5 and ABCG8 differed, such that ABCG5 was more diffuse, but ABCG8 was principally apical. Using standard deglycosylation methods, ABCG5 and ABCG8 do not appear to be glycosylated, suggesting a difference between human and mouse proteins. CONCLUSION: We report the distribution patterns of ABCG5 and ABCG8 in human tissues. Cell fractionation studies showed that both proteins co-fractionated in general, but could also be found independent of each other. As predicted, they are expressed apically in both intestine and liver, although their intracellular expression patterns are not completely congruent. These studies support the concept of heterodimerization of ABCG5 and ABCG8, but also support the notion that these proteins may have an independent function

Principles of meiotic chromosome assembly revealed in S. cerevisiae

During meiotic prophase, chromosomes organise into a series of chromatin loops emanating from a proteinaceous axis, but the mechanisms of assembly remain unclear. Here we use Saccharomyces cerevisiae to explore how this elaborate three-dimensional chromosome organisation is linked to genomic sequence. As cells enter meiosis, we observe that strong cohesin-dependent grid-like Hi-C interaction patterns emerge, reminiscent of mammalian interphase organisation, but with distinct regulation. Meiotic patterns agree with simulations of loop extrusion with growth limited by barriers, in which a heterogeneous population of expanding loops develop along the chromosome. Importantly, CTCF, the factor that imposes similar features in mammalian interphase, is absent in S. cerevisiae, suggesting alternative mechanisms of barrier formation. While grid-like interactions emerge independently of meiotic chromosome synapsis, synapsis itself generates additional compaction that matures differentially according to telomere proximity and chromosome size. Collectively, our results elucidate fundamental principles of chromosome assembly and demonstrate the essential role of cohesin within this evolutionarily conserved process

Spatio-temporal evolution of global surface temperature distributions

Climate is known for being characterised by strong non-linearity and chaotic behaviour. Nevertheless, few studies in climate science adopt statistical methods specifically designed for non-stationary or non-linear systems. Here we show how the use of statistical methods from Information Theory can describe the non-stationary behaviour of climate fields, unveiling spatial and temporal patterns that may otherwise be difficult to recognize. We study the maximum temperature at two meters above ground using the NCEP CDAS1 daily reanalysis data, with a spatial resolution of 2.5 by 2.5 degree and covering the time period from 1 January 1948 to 30 November 2018. The spatial and temporal evolution of the temperature time series are retrieved using the Fisher Information Measure, which quantifies the information in a signal, and the Shannon Entropy Power, which is a measure of its uncertainty -- or unpredictability. The results describe the temporal behaviour of the analysed variable. Our findings suggest that tropical and temperate zones are now characterized by higher levels of entropy. Finally, Fisher-Shannon Complexity is introduced and applied to study the evolution of the daily maximum surface temperature distributions.Comment: 7 pages, 4 figure

Multiphase modelling of tumour growth and extracellular matrix interaction: mathematical tools and applications

Resorting to a multiphase modelling framework, tumours are described here as a mixture of tumour and host cells within a porous structure constituted by a remodelling extracellular matrix (ECM), which is wet by a physiological extracellular fluid. The model presented in this article focuses mainly on the description of mechanical interactions of the growing tumour with the host tissue, their influence on tumour growth, and the attachment/detachment mechanisms between cells and ECM. Starting from some recent experimental evidences, we propose to describe the interaction forces involving the extracellular matrix via some concepts coming from viscoplasticity. We then apply the model to the description of the growth of tumour cords and the formation of fibrosis