Microfluidic chip for spatially and temporally controlled biochemical gradient generation in standard cell-culture Petri dishes

This paper reports the development, modeling, and testing of an original microfluidic chip capable of generating both time-evolving and spatially varying gradients in standard Petri dishes. It consists of three sets of five independently controlled parallel channels, and its architecture allows the generation of complex gradient profiles that can be flexibly positioned and dynamically altered in an open cell-chamber environment. A detailed fabrication protocol for the production of these chips using multilayer soft lithography is reported. A comprehensive computational model is also presented based on COMSOL Multiphysics software that includes both diffusion and advection of the fluid as it exits the microchannels. The results of the simulation are successfully applied to model single-channel experiments. The chip is then tested in multi-channel mode, and its ability to produce complex spatially varied concentration profiles is demonstrated. The achievement of steady state of the gradient profile in less than 5 min also allows for the dynamic variation of the profile. Finally, we apply the present chip architecture to investigate the migration of mouse neutrophils in an Interleukin-8 gradient. We report quantitatively on cell migration driven by Interleukin-8 gradient and provide migration speed distribution

Flight Test Identification and Simulation of a UH-60A Helicopter and Slung Load

Helicopter slung-load operations are common in both military and civil contexts. Helicopters and loads are often qualified for these operations by means of flight tests, which can be expensive and time consuming. There is significant potential to reduce such costs both through revisions in flight-test methods and by using validated simulation models. To these ends, flight tests were conducted at Moffett Field to demonstrate the identification of key dynamic parameters during flight tests (aircraft stability margins and handling-qualities parameters, and load pendulum stability), and to accumulate a data base for simulation development and validation. The test aircraft was a UH-60A Black Hawk, and the primary test load was an instrumented 8- by 6- by 6-ft cargo container. Tests were focused on the lateral and longitudinal axes, which are the axes most affected by the load pendulum modes in the frequency range of interest for handling qualities; tests were conducted at airspeeds from hover to 80 knots. Using telemetered data, the dynamic parameters were evaluated in near real time after each test airspeed and before clearing the aircraft to the next test point. These computations were completed in under 1 min. A simulation model was implemented by integrating an advanced model of the UH-60A aerodynamics, dynamic equations for the two-body slung-load system, and load static aerodynamics obtained from wind-tunnel measurements. Comparisons with flight data for the helicopter alone and with a slung load showed good overall agreement for all parameters and test points; however, unmodeled secondary dynamic losses around 2 Hz were found in the helicopter model and they resulted in conservative stability margin estimates

Flight Control System Development for the BURRO Autonomous UAV

Developing autonomous flying vehicles has been a growing field in aeronautical research within the last decade and will continue into the next century. With concerns about safety, size, and cost of manned aircraft, several autonomous vehicle projects are currently being developed; uninhabited rotorcraft offer solutions to requirements for hover, vertical take-off and landing, as well as slung load transportation capabilities. The newness of the technology requires flight control engineers to question what design approaches, control law architectures, and performance criteria apply to control law development and handling quality evaluation. To help answer these questions, this paper documents the control law design process for Kaman Aerospace BURRO project. This paper will describe the approach taken to design control laws and develop math models which will be used to convert the manned K-MAX into the BURRO autonomous rotorcraft. With the ability of the K-MAX to lift its own weight (6000 lb) the load significantly affects the dynamics of the system; the paper addresses the additional design requirements for slung load autonomous flight. The approach taken in this design was to: 1) generate accurate math models of the K-MAX helicopter with and without slung loads, 2) select design specifications that would deliver good performance as well as satisfy mission criteria, and 3) develop and tune the control system architecture to meet the design specs and mission criteria. An accurate math model was desired for control system development. The Comprehensive Identification from Frequency Responses (CIFER(R)) software package was used to identify a linear math model for unloaded and loaded flight at hover, 50 kts, and 100 kts. The results of an eight degree-of-freedom CIFER(R)-identified linear model for the unloaded hover flight condition are presented herein, and the identification of the two-body slung-load configuration is in progress