Phytochemical Screening and Evaluation of Antioxidant, Antidiabetic, and Antimicrobial Potential of Bergenia ligulata

Bergenia ligulata, locally known as “Pashanbheda,” belongs to the family Saxifragaceae and is a perennial herb with a short and thick stem. This plant has numerous medicinal properties including antioxidant, antidiabetic, antibacterial, and anti-inflammatory properties. The qualitative and quantitative analysis shows that the rhizome of this plant contains various phytochemical constituents. Qualitative analysis shows that it contains phenols, flavonoids, tannins, terpenoids, alkaloids, steroids, carbohydrates, and glycosides. Thirty- two bioactive compounds were identifiedduring GC-MS analysis. Dihydro-3-methylene-5-methyl-2-furanon, Stigmast-5-en-3-ol, 9-Octadecenamide(Z), 6,9-Octadecadienoic acid, methyl ester, 9,12-Octadecadienoic acid (Z, Z)-, methyl compounds have antioxidant, antibacterial and anti-inflammatory activities. The total phenol and flavonoid content from the quantitative analysis revealed a significant amount of phenol i.e., 67.1±0.02*6mg/g, and flavonoid content i.e., 89.11±0.01* mg/g. In vitro, antioxidant activities were estimated by diphenyl-1-picrylhydrazyl assay showing the highest % inhibition of 47.24±0.06* for plant extract and 41.63±0.057* for standard respectively. The in vitro, antidiabetic activitieswere estimated by alpha-amylase inhibition assay showing the highest % inhibition of 67.14±0.05* at 250 μg/ ml for methanolic plant extract and 61.42±0.01* at 250 μg/ ml for standard and in vitro antibacterial potential were detected by using Agar well diffusion process. The anti-bacterial action was found maximum in aqueous plant extract against staphylococcus aureus bacteria

Study of sociodemographic profile and pattern of gynaecological malignancies in a tertiary care center

Background: Gynaecological malignancies are second most common cancer of females after cancer breast. Carcinoma cervix is the commonest gynaecological malignancy in developing countries while in developed countries, ovarian cancer is the commonest gynaecological malignancy. Appropriate screening and timely diagnosis can save many lives. The objective of this study was to study socio-demographic profile of patients presenting with gynaecological malignancy. And to study presenting symptoms and pattern of gynaecological malignancies in themMethods: It was a retrospective record based study from December 2011 to December 2015. Various parameters from Case records of patients with gynaecological malignancies who visited outpatient department of unit IV were retrieved and analysis done.Results: In our study, most of the patients (30.52%) belonged to age group of 51-60 years, were multiparous (94.21%), Illiterate (67.3%) and belonged to rural background (65.28%). Most of the patients of cancer cervix had their presenting complaint as post-menopausal bleeding (48.38%), followed by excessive white discharge (33.87%). Majority of patients (65.26%) had cancer cervix, were in FIGO stage II .Commonest histopathological type was squamous cell carcinoma.Conclusions: Most common gynaecological malignancy in India is carcinoma cervix. Proper screening and timely diagnosis can save many lives

In Vitro Anti Inflammatory and Antioxidant Properties and GC MS Based Phytoconstituent Screening of Methanolic Fruit Berry Extracts of Gaultheria Trichophylla Royle

Gaultheria trichophylla fruit was collected from the western part of the Himalayan Mountains of Himachal Pradesh. It belongs to the family Ericaceae. In our research, the inhibition of protein albumin denaturation assay and the DPPH radical scavenging activity were analyzed to reveal the anti-inflammatory and antioxidant effects of G. trichophylla methanolic fruit extract. GC-MS analysis and qualitative and quantitative phytochemical analyses were also determined. The preliminary qualitative screening of the fruit extract identified phytoconstituents like alkaloids, flavonoids, phenols, steroids, glycosides, tannins, terpenoids, and saponins. It was found that fruit extract shows 138.88±2.346 total phenolic content and 53.96±2.049 total flavonoid content at the highest concentrations. The fruit extract possessed higher free radical scavenging activity (91.54±0.015) at 250 µg/ml concentration, having an (IC50=23.81) when compared with standard ascorbic acid (73.47±0.020) at 250 µg/ml concentration, with (IC50=136.20) and is statistically significant. The extract also exhibited higher percent inhibition of protein denaturation (82.56±0.008) at 250 µg/ml concentration, having an IC50=120.98, when compared with standard aspirin (76.47±0.014) at 250 µg/ml concentration, having an IC50=65.09 (statistically significant). GC-MS screening revealed 45 therapeutically active phytoconstituents in the extract, out of which 2,3-Dihydro-3,5-dihydroxy-6-methyl4h-pyran-4-one (15.64%); Hexadecenoic acid (15.19%); Octadec-9-enoic acid (13.78%); 9,12-Octadecadienoic acid (11.04%); 5-Hydroxymethylfurfural (7.81%); 4H-pyran-4-one, 3-hydroxy-2-methyl (7.17%); 4-Oxopentanoic acid (4.07%); 4-Methyl-6-(tetrahydropyran-2-yloxy) hex-4-enal (3.06%); 2-Decenal, (E) (2.82%); and Octadecanoic acid (2.50%) were the bioactive constituents having a high percentage of area in the chromatogram. Thus, we conclude that the fruit extract of G. trichophylla can be a powerful source of bioactive phytoconstituents of therapeutic value, anti-inflammatory, and antioxidant agents

GCMS Analysis In Vitro Antioxidant and Antidiabetic Activity of Oxyria digyna (L.) Hill

Oxyria digyna (mountain sorrel) is an edible and medicinal herb with a wide array of ethnopharmacological uses. A literature review revealed that this species is less explored for its pharmacological efficacy. Methanolic leaf extract of O. digyna was screened for antioxidant potential utilizing “2,2-diphenyl-1-picrylhydrazyl” DPPH and “Hydrogen peroxide” H202 assay, in addition, the antidiabetic potential was evaluated using enzyme alpha-amylase. Further, the bioactive compounds were analyzed through GC/MS and FTIR techniques. The antioxidant results demonstrated that the extract showed strong reducing potential for both DPPH and Hydrogen peroxide assay, plant extract revealed scavenging activity with an IC50 value of 42.55±0.7311 µg/ml for DPPH and an IC50 value of 51.77±1.855 µg/ml for H2O2. Furthermore, O. digyna showed a moderate inhibitory outcome towards alpha-amylase with an IC50 =131.02±1.90 µg/ml while the standard showed an IC50 = of 22.05±3.9 µg/ml. The extract exhibited an enormous amount of total flavonoid and phenolic. Moreover, the FTIR spectrum showed the presence of alcohol, alkanes, alkyne, aldehyde, etc. and the GC/MS study reveals the presence of sixty compounds. The most prevalent one is 9,12,15- Octadecatrienoic acid (Z, Z, Z) 38.33 %. According to our knowledge, this study is the first to validate its antidiabetic potential and identification of numerous phytoconstituents through GC/MS and identification of several functional groups employing FTIR analysis. The above finding suggests that O. digyna possess a high amount of phenols, and flavonoids, showing significant antioxidant properties, which makes it a promising source of natural antioxidant, also it can be used in food industries and for future drug synthesis. Further, the extract showed potential alpha-amylase inhibition but the potential was less, further, the active biochemical constituent can be isolated and utilized in therapeutic applications

Study of medical disorders in pregnancy among in patients at a tertiary care hospital in Haryana, India

Background: Pregnancy is a physiological condition in which various changes occur in pregnant women just to accommodate growing fetus. Pregnancy is a stress test for woman and may unmask certain underlying chronic diseases like, DM, hypertension which were silent or asymptomatic prior to pregnancy. This study was done to know the incidence, type and demographic profile of medical disorders in pregnancy among in patient at a tertiary care hospital.Methods: It was a retrospective study conducted on 578 patients of medical disorders in pregnancy admitted in a tertiary care hospital from January 2017 to December 2017. Data collected from record room and analysis done.Results: Total antenatal admissions were 4721. Incidence of medical disorders was 12.24%. Majority of women were in age group 20-30 years (65.5%). Low-parity (P0, P1). Hypertensive disorders in pregnancy was commonest reported disorder (42.3%), followed by hematological disorder (38.7%), liver disorder 5%, endocrine disorder 4.8%, epilepsy 3.8% and HIV 2.6% in present study.Conclusions: Hypertensive disorders were commonest medical disorder followed by hematological, liver, endocrine and epilepsy. All medical disorders in pregnancy to be managed by team approach

In Vivo Antidiabetic Activity and GCMS Analysis of Ethanolic Extracts of Rabdosia Rugosa (Wal ex Benth) H Hara

The current study examined the effects of an ethanolic extract of R. rugosa (Rabdosia rugosa (Wall. ex Benth.) H. Hara) on alloxan-induced diabetes rats, detailing its hypoglycemic potential and using gas chromatography-mass spectrometry to analyze its phytoconstituents. The FTIR spectrum verified the presence of various functional groups in the active ingredients. This research opted to use an extract from fresh leaves and flowers. Chichiri is the local name for the shrub R. rugosa, which belongs to the sage family (Labiatae) and is used as traditional medicine. To determine the hypoglycemic activity of plant extracts the rats were randomly assigned to one of four treatment groups for two weeks of experiments: a normal control group that received no treatment, a diabetic control group that received only alloxan (150 mg/kg BW), a drug control group that received glibenclamide (5mg/kg BW), and a treatment group that received R. rugosa extract (50 mg/kg BW). Our results demonstrated that the extract and medication group saw statistically significant improvement (p≤0.001) in body weight, blood glucose levels, lipid profile, liver and renal parameters. The GCMS analysis showed that numerous active phytoconstituents were present. Phenols, alkanes, alcohols, and other compounds were detected in the FTIR spectrum. After examining the data, we determined that the leaves and inflorescences of R. rugosa have hypoglycemic potential. Continued study of the naturally separated chemicals can aid in the creation of organic medications for diabetic treatment

Cultivation of Black Goji Berry (Lycium Ruthenicum Murr.) in the Trans Himalayan Region Ladakh Agro Technique Harvest, Yield and Cost Benefit Analysis

The present study discusses the agro technique, harvesting, yield, and economics of less explored highly medicinal plant black goji berry (Lycium ruthenicum). It was observed that black goji berries can be easily cultivated through seed, pencil-thickness hardwood stem cuttings, and rootstock. Black goji berry harvesting is a very difficult and tedious task for goji growing farmers as the berries get easily ruptured during harvesting and lose their content. To address these obstacles, DIHAR-DRDO, Leh conducted several goji berry harvesting trials deploying various techniques. Collecting fruits/berries to their full potential requires careful planning and tactics, which involves the use of an appropriate harvesting method that minimizes damage. After performing various method of harvesting, the best outcome was reported in cutting the fruit-bearing branch method as its less expensive with minimum fruit damage (5 %), time-saving, and retain the quality of fruit as compared to the other harvesting methods. An approximate cost production and net profit calculation were performed for 1011.71 square meters (02 Kanal) plantations of L. ruthenicum to their average yield of fruit production per plant. The average yield of three-year-old hardwood stem cutting and uprooted plant is 500-600 gm of fresh berry per plant on average. The economic relation to the cost production of L. ruthenicum is highly beneficial and it has all the capabilities of enhancing the socio-economy of the fragile ecosystem

Maternal and neonatal outcome in pregnancy with previous lower segment caesarean section undergoing trial of scar

Background: Women with previous LSCS often have to make a decision about mode of delivery of their second baby. As the rate of caesarean section is continuously increasing, vaginal birth after caesarean section (VBAC) is a good strategy to decrease caesarean rate. The present study was planned to assess the fetomaternal outcome in pregnancies with previous lower segment caesarean section undergoing trial of scar and to identify the factors, which can influence the outcome of trial of scar.Methods: This was a prospective observational study on 100 patients at a tertiary care institute. Pregnant women with previous LSCS were selected randomly for the study on the basis of the inclusion and exclusion criteria. Each labor monitored closely using a partogram. Decision for repeat emergency caesarean was taken by consultant. All women included in the study were followed through delivery and till discharge.Results: Out of 100 pregnant women 49 % cases had successful VBAC, 50% had emergency caesarean and one patient had laparotomy for rupture uterus. In women, who also had a prior vaginal delivery, 72% delivered vaginally, as compared to 40% of the women who did not undergo prior vaginal delivery (p value=0.003). Women who were in spontaneous labor, 59.21% delivered vaginally, whereas women who were induced, 16.6% delivered vaginally. The rate of perinatal complication was more in the patients who required an emergency CS after a failed trial. Conclusions: Our findings may encourage obstetricians to encourage VBAC in the properly screened ANC patients and decrease the rate of recommending caesarean section

An overview of funding pattern of Extramural Research projects of CSIR

560-563Geographical area- and state-university versus institutes- and scientific discipline wise distribution of CSIR funded Extramural Research projects is presented. University system in certain states needs to place more emphasis on scientific research

Phytofabrication of Silver Nanoparticles Using Trigonella foenum-graceum L. Leaf and Evaluation of Its Antimicrobial and Antioxidant Activities

Silver nanoparticles (AgNPs) were fabricated using Trigonella foenum-graceum L. leaf extract, belonging to the variety HM 425, as leaf extracts are a rich source of phytochemicals such as polyphenols, flavonoids, and sugars, which function as reducing, stabilizing, and capping agents in the reduction of silver ions to AgNPs. These phytochemicals were quantitatively determined in leaf extracts, and then, their ability to mediate AgNP biosynthesis was assessed. The optical, structural, and morphological properties of as-synthesized AgNPs were characterized using UV-visible spectroscopy, a particle size analyzer (PSA), FESEM (field emission scanning electron microscopy), HRTEM (high-resolution transmission electron microscopy), and FTIR (Fourier transform infrared spectroscopy). HRTEM analysis demonstrated the formation of spherically shaped AgNPs with a diameter of 4–22 nm. By using the well diffusion method, the antimicrobial potency of AgNPs and leaf extract was evaluated against microbial strains of Staphylococcus aureus, Xanthomonas spp., Macrophomina phaseolina, and Fusarium oxysporum. AgNPs showed significant antioxidant efficacy with IC50 = 426.25 µg/mL in comparison to leaf extract with IC50 = 432.50 µg/mL against 2,2-diphenyl-1-picrylhydrazyl (DPPH). The AgNPs (64.36 mg AAE/g) demonstrated greater total antioxidant capacity using the phosphomolybdneum assay compared to the aqueous leaf extract (55.61 mg AAE/g) at a concentration of 1100 μg/mL. Based on these findings, AgNPs may indeed be useful for biomedical applications and drug delivery systems in the future