Construção de mapa genético e análise comparativa entre cruzamentos interespecíficos de Oryza glumaepatula x Oryza sativa utilizando marcadores microssatélites.

Este trabalho objetivou: 1) desenvolver um mapa genético baseado em marcadores SSR para o cruzamento interespecífico Oryza sativa (Cica 8) x Oryza glumaepatula (RS-16); 2) avaliar a conservação da ligação e ordem dos locos SSR entre dois mapas interespecíficos, os quais possuem o parental RS-16 em comum; 3) genotipar 118 famílias na geração RC2F1 obtida a partir do cruzamento interespecífico entre Oryza sativa (Cica 8) e Oryza glumaepatula (RS-16)

BRS Ourominas: nova cultivar de arroz irrigado para Mato Grosso do Sul.

bitstream/item/24863/1/COT200254.pdfDocumento on-line

Caracterização molecular de linhagens de introgressão do cruzamento interespecífico Oryza sativa x O. glumaepatula por marcadores SSR fluorescentes.

Este trabalho teve como objetivo realizar a caracterização molecular de 114 linhagens de Introgressão RC2F9 derivadas do cruzamento O. sativa Cica-8 x O. glumaepatula RS-16

Selection of rice genotypes with greater seedling vigor under controlled conditions.

This study aimed to identify allele sources for seedling vigour in 65 rice genotypes, using the slantboard test in a germination chamber, and to determine the best parameter for evaluating seedling vigour. The experiment was arranged in the randomized block design with three replicate blocks. Root and primary leaf length data were collected from seedlings grown in the dark at 18 degrees C, 15 days after sowing and at 25 degrees C, ten days after sowing. The best performing genotypes for seedling vigour were included in Group 2, which contained red rice accessions. The vigour was best evaluated under controlled conditions (grown at 18 degrees C, 15 days after sowing) through measurements of roots and primary leaves.

Development of rice lines with gene introgression from the wild Oryza glumaepatula by the AB-QTL methodology.

Wild rice of the species Oryza glumaepatula is found Brazil and has been used to broaden the genetic basis of irrigated rice populations in Embrapa breeding programmes. The objective of this study was to demonstrate and discuss approaches used in the development of Oryza sativa lines containing genes transferred from Oryza glumaepatula, resulting in introgression lines with a broader genetic basis and high yield. First of all, genes were transferred from the wild species to cultivated rice by the AB-QTL methodology. Eighteen families were selected using QTL analysis and agronomical performance data. After the heterosis test, the families CNAi 9020 and CNAi 9024 were selected and submitted to microsatellite marker-assisted selection. Thirty-five lines were then selected with high plant vigour, high tiller and panicle number per plant, high grain yield of the main crop, and a strong regrowth capacity which makes the use of ratoons a feasible alternative

Squeezing generation and revivals in a cavity-ion system in contact with a reservoir

We consider a system consisting of a single two-level ion in a harmonic trap, which is localized inside a non-ideal optical cavity at zero temperature and subjected to the action of two external lasers. We are able to obtain an analytical solution for the total density operator of the system and show that squeezing in the motion of the ion and in the cavity field is generated. We also show that complete revivals of the states of the motion of the ion and of the cavity field occur periodically.Comment: 9 pages, 3 figure

Comparative linkage mapping of Oryza glumaepatulaand Oryza sativa interspecific crosses based on microsatellite and expressed sequence tag markers.

Molecular linkage maps representing the rice genome have been an important tool for breeding programs because they allow the elucidation of polygenic traits and are an efficient tool for monitoring wild introgressions in interspecific crosses. Common markers among rice genetic maps are important in defining the homology of chromosomes and the synteny between genomic target regions. We used 148 markers (expressed sequence tags, microsatellites and single nucleotide polymorphisms) to construct a molecular linkage map based on co-dominant markers for an interspecific backcross population using a wild rice ( Oryza glumaepatula) from Brazil and performed a comparative analysis with other interspecific maps. The comparative analysis revealed a Spearman correlation index of 0.86 for marker order conservation to a previous map constructed for an interspecific cross using the same wild parent. Approximately 90% of markers common to other interspecific maps kept the same order. These results indicate that it will be possible to generate a unique genetic map using the wild donor and that it may be a helpful tool for breeding programs because plants derived from different interspecific populations can be rapidly scanned using markers associated with useful wild traits

Avaliação de linhagens de arroz irrigado em Roraima com tipo de grão para a culinária japonesa.

Em Roraima, o cultivo do arroz representa uma das poucas cadeias produtivas efetivamente estabilizadas, com produção suficiente para abastecer o mercado interno e permitir exportação para outros Estados. No entanto, as agroindústrias locais que comercializam, basicamente, o arroz branco de classe longo-fino, podem ter na diversificação de tipos de grãos, como os demandados para a culinária japonesa, uma oportunidade de verticalização da produção e aumento de renda, já que o preço desse produto chega a ser,em média, duas a três vezes superior ao tipo local. Assim, o objetivo deste trabalho foi avaliar o desempenho produtivo de linhagens de arroz irrigado com tipo de grãos para a culinária japonesa, nas condições de cultivo de Roraima. Foram avaliadas,no período de dezembro de 2003 a março de 2004, 10 linhagens de arroz irrigado em delineamento experimental de blocos ao acaso com quatro repetições. Além de características agronômicas, foi avaliada a qualidade dos grãos. Foram realizadas análises de variância e para a comparação das estimativas das médias dos tratamentos foi aplicado o teste de Tukey em nível de 5% de probabilidade. A linhagem de arroz irrigado CNAi 9903 mostrou-se como a mais adequada para uso na culinária japonesa e apresentou boa produtividade e adaptação às condições de cultivo em Roraima.bitstream/item/28411/1/bp142009.pd

Integrating hydrogen generation and storage in a novel compact electrochemical system based on metal hydrides

A novel electrochemical system has been developed which integrates hydrogen production, storage and compression in only one device, at relatively low cost and high efficiency. The development of efficient and reliable energy storage systems based on hydrogen technology represents a challenge to seasonal storage based on renewable hydrogen. State of the art renewable energy generation systems include separate units such as electrolyser, hydrogen storage vessel and a fuel cell system for the conversion of H2 back into electricity, when required. In this work, a compact unit integrating production and storage is proposed. The developed prototype comprises a six electrode cell assembly using an AB5 type metal hydride and Ni plates as counterelectrodes, in a 35 wt% KOH solution. During charging, hydrogen is absorbed in the metal hydride and corresponding oxygen is conveyed out of the system. Conversely, in the case of discharging hydrogen stored in the metal hydride is released to an external H2 storage. In the present prototype, released hydrogen was delivered into the hydrogen storage up to a pressure of 15 Bar. Metal hydride electrodes with chemical composition LaNi4.3Co0.4Al0.3 were prepared by high frequency vacuum melting followed by high temperature annealing at 1000O C during 8 hours. X-Ray phase analysis showed typical hexagonal structure and no traces of other intermetallic compounds belonging to the La-Ni phase diagram. Thermodynamic study has been performed in a Sieverts type of apparatus produced by Labtech. Int. During cycling, charging was run at 40 A at cell voltages of 1.7 V for two hours which corresponds to C/2 charging time. Hydrogen was released by applying a constant current of 40A for two hours until cell voltage rise from 0.5 to 1.7V, at the end of the processes. The process was studied in-situ using a gas chromatograph from Agilent. It is anticipated that the device will be integrated as a combined hydrogen generator and storage unit in a stand alone system associated to a 1 kW fuel cell

Utilização de marcadores moleculares em programas de ampliação da base genética de espécies cultivadas.

Introdução; Utilização de germoplasma em programas de melhoramento genético; Determinação da variabilidade genética em acessos do banco de germoplasma; Cruzamentos amplos; Marcadores moleculares no melhoramento de plantas; Obtenção de mapas moleculares; Mapeamento de QTLs; Análise de AB-QTLs; Seleção assistida por marcadores; Considerações finais.bitstream/CNPAF/21787/1/doc_155.pd