4 research outputs found

    A study to assess the prevalence of Herpes Simplex Type 2 (HSV-2) infections in pregnant women in a tertiary care hospital

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    Aim: Assess the prevalence of Herpes Simplex Type 2 (HSV-2) infections in pregnant women in a tertiary care hospital. Methods: There were a total of one hundred pregnant women that participated in this research. 5 ml blood samples were obtained using vein puncture and placed in a sterile plain tube. These samples were then allowed to clot for 30 minutes before being centrifuged at 3000 rpm for 5 minutes. Each of the resulting serums was placed in a fresh vial, given a label, and kept at a temperature of -20 degrees Celsius until it was time for the ELISA analysis. In order to determine whether or not the serum included HSV-2 IgG antibodies, an HSV-2 specific IgG ELISA test kit was used. Results: In this research, 100 pregnant women were recruited. HSV-2 infection was found in 40% of people. The largest infected group was pregnant women under the age of 25, with 57.14 percent infected, and the lowest in older age groups. Housewives had the greatest prevalence (71.79 percent), followed by private workers (32%), while government employees had the lowest seroprevalence (8.33 percent). The relationship between viral infection and employment was statistically significant (p≤ 0.05).&nbsp

    A Study to Assess the Prevalence of Herpes Simplex Type 2 (HSV-2) Infections in Pregnant Women in A Tertiary Care Hospital

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    Aim: Assess the prevalence of Herpes Simplex Type 2 (HSV-2) infections in pregnant women in a tertiary care hospital. Methods: There were a total of one hundred pregnant women that participated in this research. 5 ml blood samples were obtained using vein puncture and placed in a sterile plain tube. These samples were then allowed to clot for 30 minutes before being centrifuged at 3000 rpm for 5 minutes. Each of the resulting serums was placed in a fresh vial, given a label, and kept at a temperature of -20 degrees Celsius until it was time for the ELISA analysis. In order to determine whether or not the serum included HSV-2 IgG antibodies, an HSV-2 specific IgG ELISA test kit was used. Results: In this research, 100 pregnant women were recruited. HSV-2 infection was found in 40% of people. The largest infected group was pregnant women under the age of 25, with 57.14 percent infected, and the lowest in older age groups. Housewives had the greatest prevalence (71.79 percent), followed by private workers (32%), while government employees had the lowest seroprevalence (8.33 percent). The relationship between viral infection and employment was statistically significant (p≤ 0.05).&nbsp

    Diagnostic accuracy of Lipoarabinomannan detection by lateral flow assay in pleural tuberculosis

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    Abstract Background Lipoarabinomannan (LAM) antigen serves as an attractive biomarker to diagnose Tuberculosis (TB). Given the limitations of current diagnostic modalities for Pleural TB, current study evaluated LAM’s potential to serve as a point-of-care test to diagnose pleural TB. Methods A cross sectional, diagnostic accuracy study was conducted during February to November 2021 in a tertiary care hospital in India. LAM antigen detection was performed on pleural fluid as well as early morning urine specimen of suspected pleural TB patients by “Alere/ Abott Determine TB LAM” lateral flow assay (LAM-LFA). The results were compared to microbiological reference standards/MRS (Mycobacterial culture or NAAT) and Composite reference standards/CRS (MRS plus Clinico-radiological diagnosis). Results A total of 170 subjects were included in the analysis, including 26 with Definite TB, 22 with Probable TB, and 122 with No TB. Compared to MRS and CRS, the sensitivity (61.54% & 45.83%) and positive predictive value (PPV) (57.14 & 78.57%) of Pleural LAM-LFA testing were found to be suboptimal, whereas the specificity (91.67% & 95.08%) and negative predictive value (NPV) (92.96% & 81.69%) of the assay were found to be good. Urinary LAM-LFA performed even worse than pleural LAM-LFA, except for its higher specificity against MRS and CRS (97.2% and 98.3%, respectively). Specificity and PPV of pleural LAM detection increased to 100% when analysed in a subgroup of patients with elevated ADA levels (receiver operating curve analysis-derived cut off value > 40 IU/ml). Conclusion Detection of LAM antigen by LFA directly from pleural fluid was found to be a useful test to predict absence of the disease if the test is negative rather than using as a POCT for diagnosis
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