In vitro development of in vivo-derived swine embryos after hyperosmotic treatment and centrifugation (the number of gilts providing embryos for this experiment was 21).

<p>In vitro development of in vivo-derived swine embryos after hyperosmotic treatment and centrifugation (the number of gilts providing embryos for this experiment was 21).</p

Blastocyst development and number of nuclei in embryos after various osmolality treatments.

<p>(^ABP<0.05; N = 160 for blastocyst development, and 113 for number of nuclei; 23 different gilts provided embryos for this experiment.).</p

Dysregulated oxidative phosphorylation pathway of deceased cloned piglets.

<p>Genes in green boxes were down-regulated in clones (fold change > 1.5). Genes in orange box and red box were upregulated in clones by more than 1.5-, and 2.0-fold, respectively. Data were analyzed by using the Fisher’s exact test (<i>p</i> < 9.95 x 10^–7, Benjamini and Hochberg FDR = 0.00019).</p

Mitochondrial <i>ND1</i> gene sequence analysis.

<p>^aStrain-specific DNA polymorphism</p><p>^bNonsense mutation</p><p>Mitochondrial <i>ND1</i> gene sequence analysis.</p

Differentially expressed mitochondrial genes in live cloned piglets.

<p>^anDNA: nuclear DNA</p><p>^bmtDNA: mitochondrial DNA</p><p>^cFold change = clone/age-matched control</p><p>Differentially expressed mitochondrial genes in live cloned piglets.</p

Primer sequences for qPCR.

<p>Primer sequences for qPCR.</p

Differentially expressed mitochondrial genes in deceased cloned piglets.

<p>^anDNA: nuclear DNA</p><p>^bmtDNA: mitochondrial DNA</p><p>^cFold change = clone/age-matched control</p><p>Differentially expressed mitochondrial genes in deceased cloned piglets.</p

Comparisons of representative gene expressions from qPCR and microarray.

<p>Black bars indicate mean gene expression data from qPCR, and gray bars from microarray. Error bars mean ± SD. Data were analyzed by one-sided t-test (*<i>p</i> < 0.05, ***<i>p</i> < 0.001).</p

Hierarchical clustering of global, nuclear DNA (nDNA)- and mitochondrial DNA (mtDNA)-encoded genes of deceased (top panel) and live clones (bottom panel) vs. age-matched controls.

<p>From left to right, clustering with all informative probes, nuclear DNA- and mitochondrial DNA-encoded probes involved in mitochondrial functions were depicted. r = correlation coefficient, red line = clones, blue line = controls, red animal ID = Duroc, blue animal ID = Large White.</p

Real time PCR validation of Illumina sequencing data for <i>WNT7A</i>, <i>FZD4</i> and <i>DVL1</i>.

<p>Real time PCR validation of Illumina sequencing data for <i>WNT7A</i>, <i>FZD4</i> and <i>DVL1</i>.</p