Draft genome sequence of the N2-fixing cyanobacterium Nostoc piscinale CENA21, isolated from the Brazilian Amazon floodplain

We announce here the draft genome sequence of Nostoc piscinale CENA21, a diazotrophic heterocyst-forming cyanobacterium isolated from the Solimões River, Amazon Basin, Brazil. It consists of one circular chromosome scaffold with 11 contigs and total size of 7,094,556 bp. Secondary metabolite annotations indicate a good source for the discovery of novel natural products. © 2016 Leão et al.This work has been supported by grants from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and from the Funda ção de Amparo à Pesquisa do Estado do Pará (BIONORTE-CNPq/FAPESPA) to M.P.C.S. This work, including the efforts of Marli Fatima Fiore and Maria Paula Cruz Schneider, was funded by MCTI | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (307045/2010-2 and 306607/2012-3). This work, including the efforts of Tiago Leão and Pedro Ivo Guimaraes, was funded by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (13425-13-7 and 13194-13-5). This work, including the efforts of Pedro Nuno Leão, was funded by Ministry of Education and Science | Fundação para a Ciência e a Tecnologia (FCT) (IF/01358/2014)

<em>Aeromonas allosaccharophila</em> strain AE59-TE2 is highly antagonistic towards multidrug-resistant human pathogens, what does its genome tell us?

Multidrug-resistant bacteria are of critical importance and a problem for human health and food preservation; the discovery of new antimicrobial substances to control their proliferation is part of the solution. This work reports on 57 antagonistic Aeromonas strains, of which 38 strains were antagonistic towards problematic human pathogens. The genome of the most antagonistic strain was sequenced and identified as Aeromonas allosaccharophila. Its genome was fully annotated and mined for genes that might explain that activity. Strain AE59-TE was antagonistic toward clinically relevant gram-negative and gram-positive multidrug-resistant bacteria, including Klebsiella pneumoniae KPC, Escherichia coli ESBL, Salmonella typhimurium, and Staphylococcus aureus MRSA. Strain AE59-TE2 was identified by multilocus sequence analysis. Genome mining identified four genes homologous to the bacteriocin, zoocin A from Streptococcus equi and a gene 98% similar to cvpA linked to colicin V production. A. allosaccharophila strain AE59-TE2 produced antimicrobial activity against a broad range of bacteria, including important gram-negative bacteria, not typically targeted by bacteriocins. Herewere described novel zoocin genes that are promising for industrial applications in the food and health sectors. Interesting and important antagonistic activity is described combined with the first detailed genomic analysis of the species Aeromonas allosaccharophila

Complete genome sequences of corynebacterium pseudotuberculosis strains 3/99-5 and 42/02-A, isolated from sheep in Scotland and Australia, respectively

Here, we report the whole-genome sequences of two ovine-pathogenic Corynebacterium pseudotuberculosis isolates: strain 3/99-5, which represents the first C. pseudotuberculosis genome originating from the United Kingdom, and 42/02-A, the second from Australia. These genome sequences will contribute to the objective of determining the global pan-genome of this bacterium