Effects of Methotrexate on IL-6alphar, VCAM-1 and NF Kappa B Expression in a Rat Model of Metabolic Syndrome

Background: In this study, we used Methotrexate (Mtx) to examine the role of immunomodulation on the activation of IL-6 and VCAM-1, which could generate a microenvironment that supports cardiovascular remodelling.Methods: Male WKY and SHR rats were separated into five groups: Control, FFR: WKY rats receiving a 10% (w/v) fructose solution during all 12 weeks, SHR, FFHR: SHR receiving a 10% (w/v) fructose solution during all 12 weeks and FFHR+Mtx (0,3 mg/kg intraperitoneal one injection per day week for 6 weeks) (n = 8 per group). Metabolic variables and systolic blood pressure were measured. Cardiac and vascular remodelling was also evaluated. To assess this, IL-6R and VCAM-1 immunostaining techniques were used.Results: The FFHR experimental model developed metabolic syndrome, vascular and cardiac remodelling, and vascular inflammation because of increased expression of IL-6 and VCAM-1. Chronic treatment with Mtx completely or partiality reversed the variables studied.Conclusions: The results demonstrated an impact on immunomodulation after mtx treatment, which included a reduction in vascular inflammation and a favourable reduction in metabolic and structural parameters.Fil: Renna, Nicolas Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Ramirez, Jésica M.. Universidad Nacional de Cuyo. Instituto de Genética; ArgentinaFil: García, Rodrigo Damián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Diez, Emiliano Raúl. Universidad Nacional de Cuyo. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Miatello, Roberto Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Instituto de Genética; Argentin

Role of Insulin Resistance in Vascular Inflammation

Cardiovascular diseases (CVDs) such as ischemic heart disease (IHD), stroke, and peripheral artery disease (PAS) are the leading causes of mortality and morbidity around the world; about 30% of global deaths and 10% of global disease burden a year are due to CVDs. In this chapter, we will analyze the classic concepts of vascular remodeling, to later expand the concepts and physiopathological mechanisms of vascular inflammation. The role of immunomodulation from IL-6R alpha and the JAK/STAT3 intracellular cascade, will be proposed as an activator of vascular remodeling mechanisms. In addition, the role of new drugs such as LCZ696 and immunomodulators involved in the local inflammatory response will also be analyzed. The concept of remodeling and vascular inflammation, which a decade ago was only important at the level of basic research, step-by-step has proven crucial in the appearance of atherosclerosis, called subclinical atherosclerosis. Even though much progress has been made in the treatment and discovery of pathophysiological mechanisms, it has not been possible to reduce of cardiovascular risk, this is perhaps, it the decade in which we can advance in this

Rol del gen supresor de tumores SMARCB1/INI1 en el desarrollo de neoplasias pediátricas.

Fil: Ramirez, Jésica. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Genética.Fil: Mampel, Alejandra. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Genética.Fil: Echeverría, María. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Genética.Fil: Calderón, Adriana. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Genética.Fil: Arce, Cecilia. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Genética.Fil: Nalda, Gonzalo. Hospital Humberto Notti (Mendoza, Argentina). Servicio de OncologíaFil: Ortiz, Leonor. Hospital Humberto Notti (Mendoza, Argentina). Servicio de OncologíaFil: Oliva, Julio. Hospital Humberto Notti (Mendoza, Argentina). Servicio de OncologíaFil: Marino, Miguel. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Laboratorio de ADNFil: Vargas, Ana. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Genética