Potential of bacteriophage K as an antimicrobial strategy against biofilms of Staphylococcus aureus in the food industry

1 poster presented at the 24th International ICFMH Conference, Food Micro 2014, 1-4 Septembre, Nantes, FranceThe potential of phage K in the removal and prevention of Staphylococcus aureus biofilms on stainless steel surfaces was examined. The host range of the phage was tested on 18 strains from the food industry. Two out of six sensitive strains were selected for study of biofilms. Immature 6-h-old biofilms were challenged wfth a wide range of multipticlties of infection {MOl, 0.01-500) for 18 h at 25ºc. A noticeable effect on cell number was appreciated at MOIs≥10, but no effect was detected at lower MOis. Subsequently, 24 h-old biofilms (with a higher cell density and a more dense extracellular matrix) were challenged too at MOIs between 0.01-3.0 for 24 hat 25ºc. Again, a significant effect was only found at MOls > 1. The effectiveness in the prevention of biofilm formation was examined by Infecting planktonlc cultures of S. aureus with sub-lethal and lethal doses of phage K (MOIs 1-10-7-10.0). A notable effect on cell number and biofilm biomass was observed from MOIs >1·10-5. The potential of phage K as a tool for biohygienization in the food Industry acting specifically against biofilms of S. aureus was thus shown. However, neither biofilm cells were completely removed nor biofilm formation was totally prevented, and a sub-population of live cells was left In both cases As a result, the effectiveness of two co-adjuvant-based strategies was subsequently assessed. Combining DNase (0,1-10 mg/ml) with phage K (MOl of 0.3-3.0) did not show an additional effect on the removal of 24 h-old-biofilms at 25°C. A 30 min pre-treatment at 37ºc aimed to enhance DNase activity also had not effect. DNase did not show any effects by itself either. In contrast, combining cis-2-decenoic acid {1 - 100 nM) with phage K (MOl of 0.03-0.3) seemed to show some effects on biofilmsN

Assessment of tolerance development in L. monocytogenes – E. coli dual – species biofilms to Pronase and Benzalkonium chloride treatments

1 poster presented at VI International Conference on Environmental, Industrial and Applied Microbiology, Barcelona, 28-30 October 2015This research was financially supported by the Spanish Ministerio de Economía y Competitividad (ENZYMONO, AGL2010-22212-C02-02). P. Rodríguez-López acknowledges the financial support from the FPI-MICINN programme (Grant number: BES-2011-050544).N

Seafood labelling in different eu countries and their compliance to eu regulations

Poster.-- Aquaculture Europe 2021, October 4-7, Funchal-Madeira, PortugalN

Characterization and potential strategies for the valorisation of the Southwest Atlantic butterfish (Stromateus brasiliensis)

10 pages, 5 tables, 1 figure.-- This article is licensed under a Creative Commons Attribution 4.0 International LicenseThe waste of fish resources constitutes a serious environmental problem that must be avoided. The valorisation of by-catch species and decreasing the discard rate constitute a more efficient and sustainable use of these marine biomasses. In this work, we characterize and propose different potential uses for Stromateus brasiliensis, another frequently discarded (≥ 90%) and poorly studied by-catch species captured in the South Atlantic Ocean (FAO 41) by trawler fishing fleets. Furthermore, in the case of this species, freezing and frozen storage of the whole fish is the only strategy currently employed for its exploitation. The results revealed that muscle from S. brasiliensis presented a high content of polyunsaturated fatty acids (20.34%) and that the concentrations of both total diacyl glyceryl ethers (2.41%) and heavy metals (Hg 0.038, Pb 0.006 and Cd 0.018 mg/kg) were below the established limits for safe human consumption. Likewise, the protein hydrolysates proved to be a good source of amino acids for human consumption or animal feeding. Minced muscle blocks could be made by a mechanical separation process of the flesh, and the composition of minced muscle did not differ much from that of the whole fish. Furthermore, this process allows the incorporation of cryoprotectants and antioxidants to extend the frozen shelf life of this fatty fish. An extraction process from mechanically mixed skin and bones yielded a good source of collagen that should not be neglectedThis work was funded by a contract with the “Cooperativa de Armadores del Puerto de Vigo”Peer reviewe

Novos métodos para garantir a autenticidade dos productos da pesca e a acuicultura: proxecto Seatraces

1 pageN

Seatraces, un proyecto para mejorar los sitemas de trazabilidad y etiquetado

3 pagesPeer reviewe

A Real Time PCR method for the authentication of common cuttlefish Sepia officinalis ) in food products

Poster.-- II International Symposium on Labelling and Authenticity of Seafood - ISLAS 2021, 20th and 21st October, VigoPeer reviewe

A Real-Time PCR Method for the Authentication of Common Cuttlefish (Sepia officinalis) in Food Products

© 2020 by the authors.Cephalopods are very relevant food resources. The common cuttlefish (Sepia officinalis) is highly appreciated by consumers and there is a lack of rapid methods for its authentication in food products. We introduce a new minor groove binding (MGB) TaqMan real-time PCR (Polymerase Chain Reaction) method for the authentication of S. officinalis in food products to amplify a 122 base pairs (bp) fragment of the mitochondrial COI (Cytochrome Oxidase I) region. Reference and commercial samples of S. officinalis showed a threshold cycle (Ct) mean of 14.40, while the rest of the species examined did not amplify, or showed a significantly different Ct (p < 0.001). The calculated efficiency of the system was 101%, and the minimum DNA quantity detected was 10−4 ng. No cross-reactivity was detected with any other species, thus, the designed method differentiates S. officinalis from other species of the genus Sepia and other cephalopod species and works for fresh, frozen, grilled, cooked and canned samples of Sepia spp. The method has proved to be reliable and rapid, and it may prove to be a useful tool for the control of fraud in cuttlefish products.This study is part of the SEATRACES project (www.seatraces.eu), funded by the EU Interreg Atlantic Area Programme (project number EAPA_87/2016).Peer reviewe

Differentiation of the production method in turbot (Scophthalmusmaximus) through the associated microbiota

Poster.-- II International Symposium on Labelling and Authenticity of Seafood – ISLAS 2021, 20th and 21st October, VigoN

Diferenzas significativas entre o rodaballo salvaxe e o de acuicultura

Poster.-- Close Encounters IIM (3rd Kind), Vigo, 23 June 2022Estudo para atopar diferenzas entre o rodaballo salvaxe e acuicultura atendendo os seguintes parámetros: Determinación dos valores de bioimpedancia (BIA), análises composición proximal do peixe, microbioma bacteriano do mucus, branquias e intestino, así como obter os datos biométricos dos individuos de estudoN