The association of miR-27a (rs895819) polymorphism with the risk of type 2 diabetes

Background and aims: Type 2 diabetes is an endocrine desease and one of the most common metabolic diseases in the world, and some environmental factors and genetic background are involved in the disease. Genetically, a large number of gene polymorphisms have been identified to be associated with this disease, but few studies have been conducted in polymorphisms miRNA and their correlation with type 2 diabetes. Among miRNAs, miR-27a involves in the predisposition to type 2 diabetes, and mutations can alter the miR-27a function and cause diabetes. The aim of the present study was to investigate the association of miR-27a (rs895819) with the risk of type 2 diabetes. Methods: In the present case-control study, 100 type 2 diabetes patients and 100 healthy individuals were randomly selected. Polymorphism miR-27a (rs895819) was investigated using PCR-RFLP method. To determine the balance or imbalance in two groups and the relationship between polymorphisms and the incidence of type 2 diabetes, Chi-square test was applied. Results: Significant differences in the genotype distribution of miR-27a (rs895819) in patients with type 2 diabetes in comparison to the healthy subjects indicated a P value equal to 0.038 (odds ratio: 2.63, 95% CI: 1.07-6.05). Conclusion: The results suggested that there is an association between miR-27a genetic variants and the incidence of type 2 diabetes. However, further studies are needed to evaluate the significance of genetic variants in different populations

Haplotype Analysis of Seven Non-Syndromeic Autosomal Recessive Hearing Loss Loci in Iranian Families

Objective: Hearing impairment is the most frequent sensorineural defect in 2 forms, syndromic and non–syndromic. The aim of this study is haplotype analysis of seven loci of non–syndromic autosomal recessive hearing loss in Iranian families. Materials & Methods: In this descriptive study, forty one Iranian families with 2 or more affected individuals segregating as an autosomal recessive non–syndromic hearing loss were selected simply and conveniently. The patients have been tested negative for the following loci, DFNB1, DFNB2, DFNB3, DFNB4, DFNB6, DFNB7/11, DFNB8/10, DFNB9, DFNB12, DFNB16, DFNB18, DFNB21, DFNB23, DFNB29 and DFNB4 previously. The subjects have been investigated additional 7 loci (DFNB22, DFNB28, DFNB30, DFNB31, DFNB36, DFNB37 and DFNB67) , to determine the prevalence of these genes involve in these loci. Homozygosity mapping was applied using number of STR (Short Tandem Repeat) markers. Results: Three families linked to the following loci DFNB28, DFNB30 and DFNB 31. Conclusion: In this research, the cause of additional 7% of non–syndromic hearing loss was determined in Iranian population