2 research outputs found
Additional file 1 of Nitrogen and sulfur-doped carbon quantum dots as fluorescent nanoprobes for spectrofluorimetric determination of olanzapine and diazepam in biological fluids and dosage forms: application to content uniformity testing
Additional file 1. Additional figures and tables
3‑Acetyl-11-keto-β-boswellic Acid-Based Hybrids Alleviate Acetaminophen-Induced Hepatotoxicity in HepG2 by the Regulation of Inflammatory and Oxidative Stress Pathways: An Integrated Approach
In an effort to develop new compounds for managing drug-induced
liver injury, we prepared 23 novel hybrids based on 3-acetyl-11-keto-β-boswellic
acid (AKBA) using various biocompatible linkers. A bioguided approach
was employed to identify the most promising hybrid. Eight compounds
exhibited superior anti-inflammatory activity compared to the parent
compound. Two of these hybrids (5b and 18) were able to reduce gene expression of TNF-α in LPS-induced
inflammation in RAW 264.7 cells, similar to dexamethasone. Subsequently,
the hepatoprotective potential of these hybrids was evaluated against
acetaminophen (APAP) toxicity in HepG2 cells at doses of 1 and 10
μM. Both hybrids effectively restored cytokine levels, which
had been elevated by APAP, to normal levels. Furthermore, they normalized
depleted superoxide dismutase and reduced glutathione levels while
significantly reducing malondialdehyde (MDA) levels. Network pharmacology
analysis suggested that AKBA-based hybrids exert their action by regulating
PI3K and EGFR pathways, activating anti-inflammatory mechanisms, and
initiating tissue repair and regeneration. Molecular docking studies
provided insights into the interaction of the hybrids with PI3K. Additionally,
the hybrids demonstrated good stability at different pH levels, following
first-order kinetics, with relatively long half-lives, suggesting
potential for absorption into circulation without significant degradation