Diversity of the meningococcal population in the Basque Country, north of Spain, 1997–2008.

<p>The ratio between the number of isolates and four parameters (number of sequence types [ST], clonal complexes, alleles and polymorphic sites) is shown among 461 invasive meningococci.</p

Annual distribution of serogroup ET15 variant of the ST11/ET37 complex.

<p>Annual distribution of serogroup B (n = 43) and C isolates (n = 63) belonging to the ET15 variant of the ET37 complex showing multilocus sequence type 11.</p

Distribution by clonal complex, sequence type and PorA type of 319 serogroup B and 123 serogroup C invasive meningococci from the Basque Country, north of Spain (1997–2008).

<p><b>Bold</b><b>type</b> indicates sequence types represented by 10 or more isolates.</p

Serogroup, sequence type and clonal complex yearly frequency among 461 available invasive meningococci from the Basque Country, north of Spain (1997–2008).

1<p>Serogroups A (n = 2), Y (n = 11) and W-135 (n = 6) are not represented in the table.</p>2<p>New STs described in this article.</p>3<p>Ratio: the higher the value, the lower the diversity (greater clonality)</p>4<p><i>I_A</i>: indicates the frequency of recombination events. <i>I_A</i> = 0 indicates frequent recombination events and <i>I_A</i>>0 indicates increasing clonality due to a lack of recombination events.</p

eBURST analysis of 461 invasive meningococci from the Basque Country, north of Spain (1997–2008).

1<p>ST: Sequence types in order of frequency (the number of isolates is given in brackets).</p>2<p>The representative phenotype was the phenotype expressed by most (>50%) of the strains in the group.</p>3<p><b>In bold</b> the new groups and singletons described in this article.</p>4<p>NT: Non-typable.</p

Number of cases and incidence rate (in brackets) of invasive <i>Neisseria meningitidis</i> of serogroup B and C by groups of age in the Basque Country, north of Spain (1997–2008).

a<p>number of cases.</p>b<p>incidence per 100,000 inhabitants.</p><p>NS: Difference not significant.</p

Bayesian skyline plot of the population growth of the subtype F cluster.

<p>The black line represents the median estimate of the effective number of infections through time (logarithmic scale) and the shaded area represents the 95% HPD credibility interval. The horizontal axis represents calendar years.</p

Distribution of viruses of the subtype F cluster sequenced by us according to city of sample collection.

<p>Distribution of viruses of the subtype F cluster sequenced by us according to city of sample collection.</p

Maximum clade credibility tree of PR-RT sequences of the subtype F Western European cluster and Galician subcluster.

<p>Nodes supported by PP = 1 and PP = 0.95–0.99 are marked with filled and unfilled circles, respectively. Colors of terminal and internal branches represent sampling locations and most probable locations of the corresponding nodes, respectively, according to the legend on the right. For the nodes corresponding to the Galician subcluster and the Western European cluster, the posterior probabilities for the most probable locations and the tMRCAs are indicated above the subtending branches (95% HPD intervals are in parentheses).</p

Maximum likelihood tree of near full-length genome sequences of viruses of the subtype F cluster.

<p>F1 subsubtype references are included in the analysis, with country of sample collection indicated with the ISO two-letter country code. The tree is rooted with an F2 subsubtype sequence. Only bootstrap values ≥80% are shown.</p