Comparison of two phenotypical methods to segregate resistant and susceptible lambs to parasitic nematodes

The objective of this study was to compare two segregation methods to select resistant and susceptible female Pelibuey lambs infected naturally with gastrointestinal nematodes (GINs) in relation to their haematological and immunological response. For 6 months, faeces and blood samples were taken fortnightly from 40 grazing 5-month-old female lambs. The lambs were classified according to two methods using faecal egg count (FEC) as a phenotypical trait. In the first (reference) method (M3SE, n = 22), resistant (RES) lambs had FEC lower than the mean – 3 standard errors, the susceptible (SUS) lambs levels higher than + 3 standard errors(n = 10) and the intermediate (INT) lambs (n = 8) were categorised by having FECs between the two values. The second method (QUM) divided the population, using quartiles, into resistant (RES; 25%), intermediate (INT; 50%), and susceptible (SUS; 25%) lambs. The agreement between both methods was estimated using the Kappa index. The packed cell volume (PCV), total plasma protein (TPP) and peripheral eosinophils (EOS) were determined for each group. Serum was used to evaluate the IgA levels. PCV and TPP values were higher (P<0.01) in the RES lambs (31.5 ± 3.4 and 6.16 ± 0.5 g/dL by QUM, respectively, and 31.5 ± 3.9 and 6.24 ± 0.49 g/dL by M3SE, respectively) than the SUS lambs (28.1 ± 4.7 and 5.94 ± 0.5 g/d, respectively, by both methods). The EOS and IgA values increased with age. M3SE and QUM were in moderate agreement (Kappa = 0.43). We concluded that the two segregation methods allowed for the identification of the same female SUS lambs, but a greater number of animals were categorised phenotypically as resistant using the M3SE method. PCV and TPP can help to identify phenotypically resistant animals

Horses’ welfare during transport

Horse transport is standard in the equine industry, and horses are transported for different reasons and activities. Therefore, knowing the factors involved during transport and horse management is critical. Horses are the most transported animals worldwide. However, this is a stressful event for horses. Factors such as vehicle type, driver's experience, length of journey, animal orientation in the vehicle, injuries, and diseases affect horses' welfare. Factors involved in horse transport might be known and considered to have adequate horse welfare.Objective: To review the literature related to horses’ welfare during transport. Approach: Transporting horses is a common practice in the equine industry and obeys to different reasons and activities. Therefore, understanding the factors that intervene during transport and management of the horses is critical. Limitations on study/implications: Horses are the most transported animals globally, this being a very stressful event. Different factors affect their welfare during this process, such as the type of vehicle, the driver’s expertise, the length of the trip, the orientation of the animal in the vehicle, lesions and diseases. Conclusions: The factors involved in horses’ transport should be known and taken into account, to ensure the horses’ welfare

Foliage of Tropical Trees and Shrubs and Their Secondary Metabolites Modify In Vitro Ruminal Fermentation, Methane and Gas Production without a Tight Correlation with the Microbiota

Ruminants, mainly cattle, contribute to greenhouse gases (GHG) emissions as methane (CH4) is produced by ruminal fermentation. Hence, various anti-methanogenic feed strategies have been studied, including the use of plants with secondary metabolites. This study evaluated in vitro ruminal fermentation metrics, microbial composition by digital droplet PCR (ddPCR) and the CH4 production of the foliage of several tropical trees and shrubs: Leucaena leucocephala, Moringa oleifera, Albizia lebbeck, Enterolobium cyclocarpum, Piscidia piscipula, Brosimum alicastrum, Lysiloma latisiliquum, Guazuma ulmifolia, Cnidoscolus aconitifolius, Gliricidia sepium and Bursera simaruba, using Cynodon plectostachyus grass as control. The results showed a wide variation in the chemical composition of the foliage, as well as in the ruminal microbiota. The crude protein (CP) content ranged from 11 to 25%, whereas the content of condensed tannins (CT) and saponins (S) was from 0.02 to 7%, and 3.2 to 6.6%, respectively. The greatest dry matter degradability (DMD) after 72 h was 69% and the least 35%, the latter coinciding with the least gas production (GP). A negative correlation was found between the CT and CH4 production, also between protozoa and fungi with the SGMT group of archaea. We concluded that the foliage of some tropical trees and shrubs has a high nutritional value and the potential to decrease CH4 production due to its CT content

Stability and Effects of Protected Palmitic Acid on In Vitro Rumen Degradability and Fermentation in Lactating Goats

Few studies have evaluated the impact of a lipid-rich diet with palmitic acid (PA) in ruminal fermentation. The objective was to evaluate the in vitro bath culture of the protected PA on the ruminal fermentative variables. Four diets were used: (a) without protected PA (nPA) and (b) inclusion of protected PA at three levels: PA3 = 3%, PA6 = 6%, and PA9 = 9% dry matter (DM). The maximum gas production occurred without including protected PA and the low gas production given with the inclusion of protected PA9 (p ≤ 0.05). Meanwhile, the inclusion with 3 and 6% of protected PA showed a higher level of gas production kinetics than the other treatments (p ≤ 0.05). DM and organic matter degradation after 72 h of incubation linearly decreased (p ≤ 0.05) with a high level of protected PA. The level of C16:0 in the protected AP increased with the higher level of supplemented AP (p ≤ 0.05). The best efficiency in propionic acid, decrease in methane, and increase in biohydrogenation (79%) occurred with the inclusion of protected PA3 (p ≤ 0.05). In conclusion, supplements with protected PA at 3 and 6% increased PA availability. The results indicate good benefits of protected PA on fermentative variables, and these doses seem ideal for future research in lactating goats

Effect of Oregano (<i>Lippia graveolens</i>) Essential Oil as a Phytogenic Feed Additive on Productive Performance, Ruminal Fermentation, and Antioxidant Activity in Lamb Meat

The objective of this study was to evaluate the inclusion of oregano essential oil (OEO) as a phytogenic feed additive in the diet of lambs on the productive performance, ruminal pH, ammoniacal nitrogen production, volatile fatty acids, nitrogen balance, carcass yield, backfat thickness, loin eye (Longissimus dorsi) area, shelf life, and antioxidant activity of the meat. Thirty-nine Suffolk × Hampshire lambs (19 ± 3.7 kg live weight) were used in a completely randomized design with three treatments (n = 13) consisting of 0, 0.02, and 0.04% OEO in the diet. The inclusion of OEO in the diet did not affect (p > 0.05) the productive performance due to the treatments; similarly, no differences (p > 0.05) were observed in the ruminal variables and nitrogen balance. Backfat thickness and loin eye area had similar results (p > 0.05), and the antioxidant activity in meat was improved (p p p p > 0.05). It is concluded that the productive performance, ruminal variables, nitrogen balance, loin eye area, backfat thickness, and carcass yield were not affected by the inclusion of OEO. However, crude protein remained stable and antioxidant activity improved, increasing the shelf life of lamb meat

Effect of Oregano (Lippia graveolens) Essential Oil as a Phytogenic Feed Additive on Productive Performance, Ruminal Fermentation, and Antioxidant Activity in Lamb Meat

The objective of this study was to evaluate the inclusion of oregano essential oil (OEO) as a phytogenic feed additive in the diet of lambs on the productive performance, ruminal pH, ammoniacal nitrogen production, volatile fatty acids, nitrogen balance, carcass yield, backfat thickness, loin eye (Longissimus dorsi) area, shelf life, and antioxidant activity of the meat. Thirty-nine Suffolk &times; Hampshire lambs (19 &plusmn; 3.7 kg live weight) were used in a completely randomized design with three treatments (n = 13) consisting of 0, 0.02, and 0.04% OEO in the diet. The inclusion of OEO in the diet did not affect (p &gt; 0.05) the productive performance due to the treatments; similarly, no differences (p &gt; 0.05) were observed in the ruminal variables and nitrogen balance. Backfat thickness and loin eye area had similar results (p &gt; 0.05), and the antioxidant activity in meat was improved (p &lt; 0.05) with the inclusion of OEO. Shelf life and crude protein content increased (p &lt; 0.05) with the inclusion of OEO in the diet and with the passing of storage days. Dry matter was affected (p &lt; 0.05) by the storage time, with a decrease observed on day 24. pH, ether extract, and ash were not affected (p &gt; 0.05). It is concluded that the productive performance, ruminal variables, nitrogen balance, loin eye area, backfat thickness, and carcass yield were not affected by the inclusion of OEO. However, crude protein remained stable and antioxidant activity improved, increasing the shelf life of lamb meat

Diagnosis and Customs Revealed by Peasants and Shepherds during the Transhumant Grazing of Pastore&ntilde;a Goats in the Mixteca of Oaxaca, Mexico

Transhumant pastoralism is a livelihood for many smallholders in the world. In Mexico, transhumant pastoralism has been practiced for 500 years and is classified as a system of transhumant goat pastoralism (TGP). The focus of the study was to identify and characterize the main goat herders of transhumance in the Mixteca-Baja of Mexico. The investigation was carried out in two phases: The first included exploring the districts to locate, contact, and live with the people involved, 13 peasants named Patrones ranging from 40 to 76 years of age. The second phase investigated the transhumant routes and the zootechnical activities of the herds. They live in five locations with an inventory of 12 thousand goats known as Pastore&ntilde;as. The TGP is a primary economic activity for all the people, and La Matanza is the main economic activity consisting of the slaughter and marketing of goats. The study revealed that the activities depend on the type of economic unit. Transhumant Pastore&ntilde;a goats garner the highest prices paid by merchants ranging from 47&ndash;70.4 USD per goat. In conclusion, the TGP is an interesting production system, and livestock activity has an essential ecological niche combining income generation and conservation of the environment

Diagnosis and Customs Revealed by Peasants and Shepherds during the Transhumant Grazing of Pastoreña Goats in the Mixteca of Oaxaca, Mexico

Transhumant pastoralism is a livelihood for many smallholders in the world. In Mexico, transhumant pastoralism has been practiced for 500 years and is classified as a system of transhumant goat pastoralism (TGP). The focus of the study was to identify and characterize the main goat herders of transhumance in the Mixteca-Baja of Mexico. The investigation was carried out in two phases: The first included exploring the districts to locate, contact, and live with the people involved, 13 peasants named Patrones ranging from 40 to 76 years of age. The second phase investigated the transhumant routes and the zootechnical activities of the herds. They live in five locations with an inventory of 12 thousand goats known as Pastoreñas. The TGP is a primary economic activity for all the people, and La Matanza is the main economic activity consisting of the slaughter and marketing of goats. The study revealed that the activities depend on the type of economic unit. Transhumant Pastoreña goats garner the highest prices paid by merchants ranging from 47–70.4 USD per goat. In conclusion, the TGP is an interesting production system, and livestock activity has an essential ecological niche combining income generation and conservation of the environment