63 research outputs found
Supplementary data for article: Ostafe, R.; ProdanoviÄ, R.; Commandeur, U.; Fischer, R. Flow Cytometry-Based Ultra-High-Throughput Screening Assay for Cellulase Activity. Analytical Biochemistry 2013, 435 (1), 93ā98. https://doi.org/10.1016/j.ab.2012.10.043
Supplementary material for: [https://doi.org/10.1016/j.ab.2012.10.043]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1606
Supplementary data for the article: Menghiu, G.; Ostafe, V.; ProdanoviÄ, R.; Fischer, R.; Ostafe, R. A High-Throughput Screening System Based on Fluorescence-Activated Cell Sorting for the Directed Evolution of Chitinase A. International Journal of Molecular Sciences 2021, 22 (6), 3041. https://doi.org/10.3390/ijms22063041.
Supplementary material:
[https://doi.org/10.3390/ijms22063041]Related to published version: [https://cherry.chem.bg.ac.rs/handle/123456789/4393
Supplementary data for the article: KovaÄeviÄ, G.; Ostafe, R.; Balaž, A. M.; Fischer, R.; ProdanoviÄ, R. Development of GFP-Based High-Throughput Screening System for Directed Evolution of Glucose Oxidase. Journal of Bioscience and Bioengineering 2019, 127 (1), 30ā37. https://doi.org/10.1016/j.jbiosc.2018.07.002
Supplementary material for: [https://doi.org/10.1016/j.jbiosc.2018.07.002]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/336
Supplementary data for article: Yu, E. H.; ProdanoviÄ, R.; Gueven, G.; Ostafe, R.; Schwaneberg, U. Electrochemical Oxidation of Glucose Using Mutant Glucose Oxidase from Directed Protein Evolution for Biosensor and Biofuel Cell Applications. Applied Biochemistry and Biotechnology 2011, 165 (7ā8), 1448ā1457. https://doi.org/10.1007/s12010-011-9366-0
Supplementary material for: [https://doi.org/10.1007/s12010-011-9366-0]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1221
Supplementary data for the article: Ostafe, R.; Prodanovic, R.; Ung, W. L.; Weitz, D. A.; Fischer, R. A High-Throughput Cellulase Screening System Based on Droplet Microfluidics. Biomicrofluidics 2014, 8 (4). https://doi.org/10.1063/1.4886771
Supplementary material for: [https://doi.org/10.1063/1.4886771]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1873
Directed evolution of cellulase from Trichoderma reesei for higher activity and development of microtiter plate assay based on cellobiose dehydrogenase
Cellulase (EC 3.2.1.4) are important enzymes in food, paper, textile, detergent and biofuel industries. Most cellulases have low activity and stability. Improving these properties would have substantial impact on numerous industrial processes. Enzymatic properties can be improved by directed evolution, but the screening process is the limiting step. Coupled cellulase assay has been developed in order to improve the screening process. This method does not require boiling samples and allows rapid screening of mutants in a microtiter plate. The aim of this study was to establish enzyme coupled assay where cellulase first hydrolyzes carboxymethylcellulose (CMC), and cellobioses dehydrogenase (CBDH) and dichlorophenolindophenol (DCPIP) is used subsequently for detection of reducing ends
Nanobiokatalizatori za biogorivne Äelije i biosenzorne sisteme
This overview summarizes the application of enzymes in the manufacture and design of biofuel cells and biosensors. The emphasis will be put on the protein engineering techniques used for improving the properties of enzymes such as nanobiocatalysts, e.g. immobilization orientation, stability, activity and efficiency of electron transfer between immobilized enzymes and electrodes. Some possible applications in the military and some future designs of these electric devices will be discussed as well.U ovom preglednom Älanku je sumirana primena enzima u proizvodnji i dizajnu biogorivnih Äelija i biosenzora. Naglasak u pregledu literature je stavljen na tehnike proteinskog inžinjeringa, koje se koriste za poboljÅ”anje osobina enzima u nanobiokatalizatorima kao Å”to su orijentacija kod imobilizacije, stabilnost, aktivnost i efikasnost transfera elektrona izmeÄu imobilizovanog enzima i elektrode. Na kraju pregleda je dato nekoliko primera moguÄe primene u vojsci. Nanobiokatalizatori su biokatalizatori u obliku enzima ili Äelija imobilizovani na nanomaterijalima. Koriste se kao sastavni elementi gorivnih Äelija u vidu imobilizovanih oksidoreduktaza na elektrodama. Na anodi se uz pomoÄ enzima oksiduju hemijska jedinjenja i elektroni predaju elektrodi, dok se na katodi elektroni uz pomoÄ druge oksidoreduktaze prebacuju sa elektrode na vodu ili kiseonik. Enzimi koji se koriste na anodi su glukoza oksidaza, formaldehid dehidrogenaza, alkohol dehidrogenaza i druge oksidaze Å”eÄera. Na katodi se uglavnom koriste lakaze, bilirubin oksidaza, peroksidaze i citohrom c oksidaza. ZahvaljujuÄi razvoju nanotehnologije razvijaju se i minijaturne biogorivne Äelije koje proizvode elektriÄnu energiju za implantirane medicinske ureÄaje (insulinske pumpe, pejsmejkere, biosenzore) koristeÄi glukozu i kiseonik iz ljudske krvi. Biosenzori predstavljaju ureÄaje koji se sastoje iz bioloÅ”ke komponente, transducera i elektriÄne komponente. Oni pretvaraju koncen traciju hemijske supstance u elektriÄni signal i koriste se za analitiku. Kao bioloÅ”ka komponenta se mogu koristiti enzimi, monoklonska antitela, nukleinske kiseline i lipidi. Enzimska logiÄka kola predstavljaju kombinaciju razliÄitih biosenzora (enzimskih reakcija) koji mere nekoliko ulaznih parametara i na osnovu njih daju odgovarajuÄi izlazni signal. KoristeÄi znanja kompjuterske tehnologije enzimskim logiÄkim kolima mogu se simulirati AND, OR, XOR, NOR, NAND, INHIB i XNOR logiÄka kola. Za poboljÅ”anje osobina biokatalizatora u cilju efikasnije primene u bioelektrokatalizi koriste se tehnike proteinskog inžinjeringa kao Å”to su racionalni dizajn i dirigovana evolucija. Dirigovana evolucija koristi iterativne korake mutiranja i selekcije, kako bi biokatalizator evoluirao u pravcu koji nam je potreban. Najsporiji stupanj u ovoj tehnologiji predstavlja 'skrining', te se u novije vreme pomoÄu protoÄne citometrije i mikrofluidike pokuÅ”avaju razviti nove metode visoko propusnog skrininga. U literaturi opisani primeri dirigovane evolucije glukoza oksidaze, glukoza dehidrogenaze, formaldehid dehidrogenaze, laktat dehidrogenaze, peroksidaze i lakaze. Kombinacijom enzimskih logiÄkih kola i mikrofluidne tehnologije se pokuÅ”avaju napraviti laboratorije na Äipu koje bi omoguÄile kontinuirano praÄenje zdravstvenog stanja vojnika na bojnom polju i u sluÄaju Å”oka (ranjavanja) primenu odgovarajuÄe terapije u toku prvih 30 minuta od povrede. To bi obezbedilo veÄi stepen preživljavanja vojnika u ratu. TakoÄe upotrebom enzimskih logiÄkih kola i antitela moguÄe je postiÄi uskladiÅ”tenje i Å”ifrovanje informacija, kao i zaÅ”titu lozinkom, odgovarajuÄih elektronskih ureÄaja kao Å”to su biogorivne Äelije Razvoj nanotehnologije, proteinskog inžinjeringa i molekularnog raÄunarstva otvara vrata novim moguÄnostima u proizvodnji i dizajnu biogorivnih Äelija i bisenzorskih sistema, kao i u skladiÅ”tenju i zaÅ”titi informacija
Supplementary data for the article: KovaÄeviÄ, G.; Ostafe, R.; Fischer, R.; ProdanoviÄ, R. Influence of Methionine Residue Position on Oxidative Stability of Glucose Oxidase from Aspergillus Niger. Biochemical Engineering Journal 2019, 146, 143ā149. https://doi.org/10.1016/j.bej.2019.03.016
Supplementary material for: [https://doi.org/10.1016/j.bej.2019.03.016]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2881
Supplementary data for article: Ostafe, R.; ProdanoviÄ, R.; Commandeur, U.; Fischer, R. Flow Cytometry-Based Ultra-High-Throughput Screening Assay for Cellulase Activity. Analytical Biochemistry 2013, 435 (1), 93ā98. https://doi.org/10.1016/j.ab.2012.10.043
Supplementary material for: [https://doi.org/10.1016/j.ab.2012.10.043]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1606
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