(A) A schematic representation of the WT and mutant alleles and the targeting vector

E, EcoRI; H, HindIII; B, BamHI; X, XbaI; N, NotI; K, Kpn. (B) Southern blot analysis of tail DNA from WT (), heterozygote () and knockout () mouse littermates. The presence of the 3.2-kb wild-type fragment and the 4.8-kb recombinant fragments are seen in genomic DNA digested with HindIII. (C) Northern blot analysis of BSP mRNA expression in calvaria and femur bones of young and adult and mice. L32, housekeeping gene.<p><b>Copyright information:</b></p><p>Taken from "Bone sialoprotein plays a functional role in bone formation and osteoclastogenesis"</p><p></p><p>The Journal of Experimental Medicine 2008;205(5):1145-1153.</p><p>Published online 12 May 2008</p><p>PMCID:PMC2373846.</p><p></p

Picture (A), measurements of body length (B), body weight (C), and percentage of fat mass (D) of and mice

μCT scout images of whole femurs (E), and femur length (F) at 4 and 12 mo of age for WT () and mutant () female mice; similar results were obtained with males (not depicted). Two-dimensional images of midshaft sections in specimens from both genotypes (G) and cortical thickness (H) at 4 and 12 mo of age for and male and female mice. Values are the mean ± the SEM of 6–10 samples. **, P < 0.01; ****, P < 0.0001 versus matched WT; #, P < 0.05 versus matched females.<p><b>Copyright information:</b></p><p>Taken from "Bone sialoprotein plays a functional role in bone formation and osteoclastogenesis"</p><p></p><p>The Journal of Experimental Medicine 2008;205(5):1145-1153.</p><p>Published online 12 May 2008</p><p>PMCID:PMC2373846.</p><p></p

Micrographs and quantification of total colony forming units-fibroblasts (CFU-F; B), unmineralized ALP colonies (CFU-ALP; C) and mineralized ALP colonies (CFU-O; D) in bone marrow stromal cell cultures of female mutant and WT mice

Values are the mean ± the SEM of 8–15 wells. (E) Micrographs of and female spleen cells grown with RANKL+M-CSF and stained for TRAP activity (TRAP); (F) number of TRAP cells; (G) number of osteoclasts (TRAP cells with ≥3 nuclei) formed in spleen cell and bone marrow cultures. Values are the mean ± the SEM of 12 wells. (H) Number of resorption pits on dentine slices plated with and differentiated osteoclasts; values are the mean ± the SEM of six slices. ***, P < 0.001; ****, P < 0.0001 versus matched WT.<p><b>Copyright information:</b></p><p>Taken from "Bone sialoprotein plays a functional role in bone formation and osteoclastogenesis"</p><p></p><p>The Journal of Experimental Medicine 2008;205(5):1145-1153.</p><p>Published online 12 May 2008</p><p>PMCID:PMC2373846.</p><p></p