13 research outputs found
Real time acidification rate as a live cell parameter.
<p>CB derived hMSC show significant increased metabolic activity under hypoxic situation compared to BM- and CD105-purified CB-derived hMSC.</p
Heart functions 6 weeks after MI.
<p><b><i>A.</i></b> Recovery of cardiac performance shows improvement for hearts with implanted human BM- and CD105-purified CB-hMSC compared to MI-CB hearts. <b><i>B.</i></b> Left ventricular functions at both baseline and stress condition assessed by catheterization.</p
Immunophenotypic analysis of hMSC from different origins.
<p>n = 3; mean [%]±SEM.</p><p>*p<0,05 compared to AT, BM.</p
CD105 cell isolated from BM hMSC were transiently transfected with GFP, antisense-Oligodesoxynukleotide (ODN-CD105), scramble ODN.
<p><b><i>A.</i></b> Transfection efficiency is shown on the upper picture (GFP) <b><i>B.</i></b> Silencing of CD105 blocks BM-hMSC tube formation compared to nature and scrambled group. The BM-hMSC were embedded in Matrigel and incubated in EGM-2 seven days after transfection and then imaged. <i>C.</i> Cell proliferation was analyzed at 24, 48 and 96 h after the ODN-CD105 and scramble ODN transfection by MTT assay.</p
CD105 expression in CB105<sup>+</sup> hMSC over the culture time.
<p>n = 3; mean [%]±SEM.</p
Late cardiomyocytes apoptosis.
<p><b><i>A.</i></b> Representative immunostaining for TUNEL (green) and cardiac troponin (red) at the BZ 6 weeks after MI. <b><i>B.</i></b> Cardiomyocytes apoptosis was significantly reduced in the BZ in MI-BM and MI-CB105<sup>+</sup> compared to MI-C.</p
Infarction size 6 weeks after MI.
<p><b><i>A.</i></b> Representative ventricular cross sections of heart level c. <b><i>B.</i></b> Ratio of infarction size to entire LV is significantly decreased in MI-BM and MI-CB105<sup>+</sup> compared to MIC.</p
Capillary density 6 weeks after MI.
<p><b><i>A.</i></b> Representative endothelial CD31 staining at the infarction border zone of level c sections. <b><i>B.</i></b> Capillary density in both the RA and the BZ of the LV is significantly higher in MI-AT, MI-BM and MI-CB105<sup>+</sup> compared to MIC.</p
Identification of transplanted hMSC in infracted myocardium.
<p>6 weeks after MI: <b><i>A–C.</i></b> Representative immunofluorescent micrographs of hearts transplanted with hMSC. <b><i>A</i></b> transplanted hMSC could be identified in infarcted myocardium <b><i>B.</i></b> A number of hMSC (Arrows, human nuclei in green) were co-localized with CD31 positive cells (red). <b><i>C.</i></b> Occasionally hMSC (Arrow, human nuclei in green) co-localized with cardiac troponin positive cell (red). (Confocal image, original magnification 630×) <b><i>D.</i></b> Quantitative real-time PCR analysis for human GAPDH expression level at different infarction sections: MI-BM and MI-CB105<sup>+</sup> hearts show significantly higher localisation of human cells in the middle and apex section.</p
Fibrosis 6 weeks after MI.
<p><b><i>A.</i></b> Representative Fast Green FCF (myocytes)/Sirius Red (fibrosis) stainings at the BZ. <b><i>B.</i></b> Significantly decrease of collagen deposition has been shown in both the RA and the BZ in MI-CB105<sup>+</sup> compared to MI-CB and MI-C.</p