Design and Fabrication of a Gear Box Motor Current Analysis System

To achieve reliable and cost effective diagnosis, Motor current signature analysis is used to investigate the use of an induction motor as a transducer to indicate the faults in multistage gearbox via analyzing supply parameters such as phase current and instantaneous power. In gearboxes, load fluctuations on the gearbox and gear defects are two major sources of vibration. Further at times, measurement of vibration in the gearbox is not easy because of the inaccessibility in mounting the vibration transducers. This analysis system can be used for measuring the characteristics for a perfectly working gearbox and use the data as a standard for measuring faults and defects in other gearboxes. The objective of this paper is to design and fabricate a gearbox motor current analysis system at different gear operations for a constant load. Steady load conditions on the gearbox are tested for current signatures during different gear operations. The motor current analysis system can be used further to specify mainly faults in the gear, misalignment of meshed gears, and loss of contact of the gears

Expression Efficiency of Multiple Il9 Reporter Alleles Is Determined by Cell Lineage

Generation of allelic gene reporter mice has provided a powerful tool to study gene function in vivo. In conjunction with imaging technologies, reporter mouse models facilitate studies of cell lineage tracing, live cell imaging, and gene expression in the context of diseases. Although there are several advantages to using reporter mice, caution is important to ensure the fidelity of the reporter protein representing the gene of interest. In this study, we compared the efficiency of two Il9 reporter strains Il9citrine and Il9GFP in representing IL-9-producing CD4+ TH9 cells. Although both alleles show high specificity in IL-9-expressing populations, we observed that the Il9GFP allele visualized a much larger proportion of the IL-9-producing cells in culture than the Il9citrine reporter allele. In defining the mechanistic basis for these differences, chromatin immunoprecipitation and chromatin accessibility assay showed that the Il9citrine allele was transcriptionally less active in TH9 cells compared with the wild-type allele. The Il9citrine allele also only captured a fraction of IL-9-expressing bone marrow-derived mast cells. In contrast, the Il9 citrine reporter detected Il9 expression in type 2 innate lymphoid cells at a greater percentage than could be identified by IL-9 intracellular cytokine staining. Taken together, our findings demonstrate that the accuracy of IL-9 reporter mouse models may vary with the cell type being examined. These studies demonstrate the importance of choosing appropriate reporter mouse models that are optimal for detecting the cell type of interest as well as the accuracy of conclusions