Immune cells control skin lymphatic electrolyte homeostasis and blood pressure

The skin interstitium sequesters excess Na+ and Cl- in salt-sensitive hypertension. Mononuclear phagocyte system (MPS) cells are recruited to the skin, sense the hypertonic electrolyte accumulation in skin, and activate the tonicity-responsive enhancer-binding protein (TONEBP, also known as NFAT5) to initiate expression and secretion of VEGFC, which enhances electrolyte clearance via cutaneous lymph vessels and increases eNOS expression in blood vessels. It is unclear whether this local MPS response to osmotic stress is important to systemic blood pressure control. Herein, we show that deletion of TonEBP in mouse MPS cells prevents the VEGFC response to a high-salt diet (HSD) and increases blood pressure. Additionally, an antibody that blocks the lymph-endothelial VEGFC receptor, VEGFR3, selectively inhibited MPS-driven increases in cutaneous lymphatic capillary density, led to skin Cl- accumulation, and induced salt-sensitive hypertension. Mice overexpressing soluble VEGFR3 in epidermal keratinocytes exhibited hypoplastic cutaneous lymph capillaries and increased Na+, Cl-, and water retention in skin and salt-sensitive hypertension. Further, we found that HSD elevated skin osmolality above plasma levels. These results suggest that the skin contains a hypertonic interstitial fluid compartment in which MPS cells exert homeostatic and blood pressure-regulatory control by local organization of interstitial electrolyte clearance via TONEBP and VEGFC/VEGFR3-mediated modification of cutaneous lymphatic capillary function

Fast solution of the Shafranow equation for comparison with experimental observations

A method is described to construct a polynome approximation of SHAFRANOWs equilibrium conditions from given distributions of the current density. For the flux function a polynomial of a certain degree is assumed. The partial differential equation which connects theflux function and the current distribution is used to determine the coefficients of the polynomial. An accuracy test shows whether the assumed degree of this polynomial is sufficiently high. The plasma pressure flux function. For assumed, and the given current pressure part and the current flux aspect ratio (major radius / minor too great. From the calculated total plasma force and the elctromagnetic force it can be seen, polynomials is sufficiently high.and the current function are related to theboth a polynomial with respect to the flux isdensity is splitted into thepart . This can be done, if theradius of the plasma) is nottotal plasma force and thewhethe

High-salt diet causes expansion of the lymphatic network and increased lymph flow in skin and muscle of rats

OBJECTIVE: A commonly accepted pivotal mechanism in fluid volume and blood pressure regulation is the parallel relationship between body Na+ and extracellular fluid content. Several recent studies have, however, shown that a considerable amount of Na+ can be retained in skin without commensurate water retention. Here, we asked whether a salt accumulation shown to result in VEGF (vascular endothelial growth factor)-C secretion and lymphangiogenesis had any influence on lymphatic function. APPROACH AND RESULTS: By optical imaging of macromolecular tracer washout in skin, we found that salt accumulation resulted in an increase in lymph flow of 26% that was noticeable only after including an overnight recording period. Surprisingly, lymph flow in skeletal muscle recorded with a new positron emission tomography/computed tomography method was also increased after salt exposure. The transcapillary filtration was unaffected by the high-salt diet and deoxycorticosterone-salt treatment, suggesting that the capillary barrier was not influenced by the salt accumulation. A significant reduction in lymph flow after depletion of macrophages/monocytes by clodronate suggests these cells are involved in the observed lymph flow response, together with collecting vessels shown here to enhance their contraction frequency as a response to extracellular Na+. CONCLUSIONS: The observed changes in lymph flow suggest that the lymphatics may influence long-term regulation of tissue fluid balance during salt accumulation by contributing to fluid homeostasis in skin and muscle. Our studies identify lymph clearance as a potential disease-modifying factor that might be targeted in conditions characterized by salt accumulation like chronic kidney disease and salt-sensitive hypertension

Experimental validation of fringing field effects for the multipactor phenomenon

The multipactor effect is a constraining phenomenon in the design of RF components. Due to an increasing number of channels and power levels for multicarrier signals, specific requirements of design must be taken into account in order to prevent the multipactor effect. The current methods of analysis are based on the Hatch and Williams diagrams, which provide the multipaction threshold voltage for a parallel plate geometry as a function of the frequency gap product. However, whereas experimental results have shown good correlation with the theoretical charts for the parallel plate geometry, discrepancies have been found for complex geometries such as irises