219 research outputs found
Regge behaviour of distribution functions and t and x-evolutions of gluon distribution function at low-x
In this paper t and x-evolutions of gluon distribution function from
Dokshitzer-Gribov-Lipatov-Altarelli-Parisi(DGLAP) evolution equation in leading
order(LO) at low-x, assuming the Regge behaviour of quark and gluon at this
limit, are presented. We compare our results of gluon distribution function
with MRST 2001, MRST 2004 and GRV '98 parameterizations and show the
compatibility of Regge behaviour of quark and gluon distribution functions with
perturbative quantum chromodynamics(PQCD) at low-x. We also discuss the
limitations of Taylor series expansion method used earlier to solve DGLAP
evolution equations, in the Regge behaviour of distribution functions.Comment: 19 pages, 7 figure
Biodegradable Eri silk nanoparticles as a delivery vehicle for bovine lactoferrin against MDA-MB-231 and MCF-7 breast cancer cells
This study used the Eri silk nanoparticles (NPs) for delivering apo-bovine lactoferrin (Apo-bLf) (~2% iron saturated) and Fe-bLf (100% iron saturated) in MDA-MB-231 and MCF-7 breast cancer cell lines. Apo-bLf and Fe-bLf-loaded Eri silk NPs with sizes between 200 and 300 nm (±10 nm) showed a significant internalization within 4 hours in MDA-MB-231 cells when compared to MCF-7 cells. The ex vivo loop assay with chitosan-coated Fe-bLf-loaded silk NPs was able to substantiate its future use in oral administration and showed the maximum absorption within 24 hours by ileum. Both Apo-bLf and Fe-bLf induced increase in expression of low-density lipoprotein receptor-related protein 1 and lactoferrin receptor in epidermal growth factor (EGFR)-positive MDA-MB-231 cells, while transferrin receptor (TfR) and TfR2 in MCF-7 cells facilitated the receptor-mediated endocytosis of NPs. Controlled and sustained release of both bLf from silk NPs was shown to induce more cancer-specific cytotoxicity in MDA-MB-231 and MCF-7 cells compared to normal MCF-10A cells. Due to higher degree of internalization, the extent of cytotoxicity and apoptosis was significantly higher in MDA-MB-231 (EGFR+) cells when compared to MCF-7 (EGFR-) cells. The expression of a prominent anticancer target, survivin, was found to be downregulated at both gene and protein levels. Taken together, all the observations suggest the potential use of Eri silk NPs as a delivery vehicle for an anti-cancer milk protein, and indicate bLf for the treatment of breast cancer
Impact of tranexamic acid on coagulation parameters in patients undergoing total knee replacement surgeries under tourniquet: an observational study
Background: A growing body of evidence has shown Tranexamic Acid (TXA) is effective in decreasing perioperative blood loss and transfusion requirements in both primary and revision joint arthroplasty. TXA is a synthetic drug which limits blood loss through inhibition of fibrinolysis and clot degradation. It helps reduce requirement of colloids and crystalloids and hence provides better haemodynamic stability. The aim of this study was to detect the effect of tranexamic acid on coagulation parameters and effect on bleeding in knee replacement surgeries performed under tourniquet.Methods: Patients undergone surgeries of Total Knee Replacement (TKR) performed under tourniquet were included in the study. A single dosage of 20 mg/kg per body weight of tranexamic acid was administered after application of a tourniquet. Three times blood sample was collected, and coagulation parameters were recorded and compared. The first sample was collected at the time of TXA injection and application of a tourniquet, second after 4 hours and third after 24 hours post TXA injection. Coagulation parameters noted were analyzed using Statistical analysis by SPSS software. All parameters were compared in relation to baseline i.e. at the time of TXA injection.Results: On comparison of demographic profile, morbidity, sofa score and hemodynamic parameters there was the insignificant difference (P > 0.05). Repeated measures of ANOVA at 95% Confidential Interval P value was 0.000 which is less than the significant level that is 0.05 so that value of Platelet Function (PF), Activated Coagulation Time (ACT) and Clot Rate (CR) at 0 hrs, 04 hrs and 24 hrs was statistically significant. Correlation between blood loss and difference of the value of ACT at 0 hrs and 04 hrs is a small negative correlation but statistically nonsignificant (P value is 0.359).Conclusions: After TXA administration there is a change in coagulation parameters like an Activated Coagulation Time (ACT), Platelet Function (PF), and Clot Rate (CR) measured at three intervals, hence it can be a guide to detect early derangement in the coagulation profile in a patient undergoing knee replace surgery. TXA correlation between blood loss with changes in parameters of coagulation i.e. ACT, PF and CR were noted but not significant
Lowering solar mixing angle in inverted hierarchy without charged lepton corrections
In the present work, the inverted hierarchical neutrino mass model which is
characterised by opposite CP parity in the first two mass eigenvalues
, is studied in order to lower the predicted value of solar
mixing angle , from the tri-bimaximal mixing (TBM), without
sacrificing the conditions of maximal atmospheric mixing angle and zero reactor
angle. The present attempt is different from the earlier approach where the
correction from the charged lepton mass matrix is included in the leptonic
mixing matrix to lower the prediction on solar mixing angle. The lowering of
the solar mixing angle without charged lepton correction, can be obtained
through the variation of the input value of a flavour twister term present in
the texture of neutrino mass matrix having a 2-3 symmetry. The present analysis
agrees with the latest experimental bounds on neutrino mass parameters and also
represents an important result on the survival of the inverted hierarchical
neutrino mass models having opposite CP parity in the first two eigenvalues.Comment: 10 pages, two figures. Accepted for publication in Journal of Physics
G:Nuclear and Particle Physic
Deviation from tri-bimaximal mixings in two types of inverted hierarchical neutrino mass models
An attempt is made to explore the possibility for deviations of solar mixing
angle () from tri-bimaximal mixings, without sacrificing the
predictions of maximal atmospheric mixing angle () and zero
reactor angle (). We find that the above conjecture can be
automatically realised in the inverted hierarchical neutrino mass model having
2-3 symmetry, in the basis where charged lepton mass matrix is diagonal. For
the observed ranges of and \bigtriangleup m^2_{23],
we calculate the predictions on for
different input values of the parameters in the neutrino mass matrix. We also
observe a possible crossing over from one type of inverted hierarchical model
having same CP parity (Type-IHA) to other type having opposite CP parity
(Type-IHB). Such neutrino mass matrices can be obtained from the canonical
seesaw formula using diagonal form of Dirac neutrino mass matrix and
non-diagonal texture of right-handed Majorana mass matrix, and may have
important implications in model building using discrete as well as non-abelian
symmetry groups.Comment: 13 pages, 7 figure
Regge behaviour of distribution functions and evolution of gluon distribution function in Next-to-Leading order at low-x
Evolution of gluon distribution function from
Dokshitzer-Gribov-Lipatov-Altarelli-Parisi (DGLAP) evolution equation in
next-to-leading order (NLO) at low-x is presented assuming the Regge behaviour
of quarks and gluons at this limit. We compare our results of gluon
distribution function with MRST2004, GRV98LO and GRV98NLO parameterizations and
show the compatibility of Regge behaviour of quark and gluon distribution
functions with perturbative quantum chromodynamics (PQCD) at low-x.Comment: 12 pages, 4 figure
Obesity-induced insulin resistance in human skeletal muscle is characterised by defective activation of p42/p44 MAP kinase
Insulin resistance (IR), an impaired cellular, tissue and whole body response to insulin, is a major pathophysiological defect of type 2 diabetes mellitus. Although IR is closely associated with obesity, the identity of the molecular defect(s) underlying obesity-induced IR in skeletal muscle remains controversial; reduced post-receptor signalling of the insulin receptor substrate 1 (IRS1) adaptor protein and downstream effectors such as protein kinase B (PKB) have previously been implicated. We examined expression and/or activation of a number of components of the insulin-signalling cascade in skeletal muscle of 22 healthy young men (with body mass index (BMI) range, 20â37 kg/m2). Whole body insulin sensitivity (M value) and body composition was determined by the hyperinsulinaemic (40 mU. minâ1.mâ2.), euglycaemic clamp and by dual energy X-ray absorptiometry (DEXA) respectively. Skeletal muscle (vastus lateralis) biopsies were taken before and after one hour of hyperinsulinaemia and the muscle insulin signalling proteins examined by western blot and immunoprecipitation assay. There was a strong inverse relationship between M-value and BMI. The most striking abnormality was significantly reduced insulin-induced activation of p42/44 MAP kinase, measured by specific assay, in the volunteers with poor insulin sensitivity. However, there was no relationship between individuals' BMI or M-value and protein expression/phosphorylation of IRS1, PKB, or p42/44 MAP kinase protein, under basal or hyperinsulinaemic conditions. In the few individuals with poor insulin sensitivity but preserved p42/44 MAP kinase activation, other signalling defects were evident. These findings implicate defective p42/44 MAP kinase signalling as a potential contributor to obesity-related IR in a non-diabetic population, although clearly multiple signalling defects underlie obesity associated IR
Recommended from our members
Expression of putative targets of immunotherapy in acute myeloid leukemia and healthy tissues
Fabrication and characterization of Eri silk fibers-based sponges for biomedical application
Cocoon-derived semi-domesticated Eri silk fibers still lack exploitation for tissue engineering applications due to their poor solubility using conventional methods. The present work explores the ability to process cocoon fibers of non-mulberry Eri silk (Samia/Philosamia ricini) into sponges through a green approach using ionic liquid (IL) â 1-buthyl-imidazolium acetate as a solvent. The formation of Ă²-sheet structures during Eri silk/IL gelation was acquired by exposing the Eri silk/IL gels to a saturated atmosphere composed of two different solvents: (i) isopropanol/ethanol (physical stabilization) and (ii) genipin, a natural crosslinker, dissolved in ethanol (chemical crosslinking). The sponges were then obtained by freeze-drying. This approach promotes the formation of both stable and ordered non-crosslinked Eri silk fibroin matrices. Moreover, genipin-crosslinked silk fibroin sponges presenting high height recovery capacity after compression, high swelling degree and suitable mechanical properties for tissue engineering applications were produced. The incorporation of a model drug â ibuprofen â and the corresponding release study from the loaded sponges demonstrated the potential of using these matrices as effective drug delivery systems. The assessment of the biological performance of ATDC5 chondrocyte-like cells in contact with the developed sponges showed the promotion of cell adhesion and proliferation, as well as extracellular matrix production within two weeks of culture. Spongesâ intrinsic properties and biological findings open up their potential use for biomedical applications.The authors SSS, DSC, MBO, NMO acknowledge financial support
from Portuguese Foundation for Science and Technology â
FCT (Grants SFRH/BPD/45307/2008, SFRH/BPD/85790/2012,
SFRH/BD/71396/2010 and SFRH/BD/73172/2010, respectively),
ââFundo Social Europeuâ â FSE, and ââPrograma Diferencial de Potencial
Humano POPHâ. This work is also financially supported by the European Union Seventh Framework Programme (FP7/2007-2013)
under grant agreement n REGPOT-CT2012-316331-POLARIS and
from Fundação para a Ciência e Tecnologia (FCT) through the project
ENIGMA â PTDC/EQU-EPR/121491/2010. The laboratory work
of SCK is supported by Department of Biotechnology and Indian
Council of Medical Research, Govt of India. SCK and RLR acknowledge
their short visits either Institutes. SCK is also grateful to 3B´ s
Research Group- Biomaterials, Biodegradables and Biomimetics,
University of Minho, Portugal for providing facilities during his
short visit
- âŚ