3CCD and infrared (IR) imaging. Top) Representative grayscale 3CCD images of a hind limb, and Middle) representative grayscale infrared images of a hind limb at (1) baseline, (2) maximum ischemia, (3) 10 minutes post-reperfusion, and (4) 30 minutes post-reperfusion.

<p>Bottom left) Profile of R-B values, derived from 3CCD imaging, over the course of 3.5 hours of ischemia and 30 minutes of reperfusion. Bottom right) Profile of IR values and corresponding mean leg temperature over the course of 3.5 hours of ischemia and 30 minutes of reperfusion. The time points mentioned previously (1–4) are noted.</p

Outcome based comparison of various metrics.

<p>Values are means +/- the standard deviation for animals that 1) recovered full locomotion, and 2) did not recover full locomotion. Differences approaching statistical significance (p-value ≈ 0.1) are indicated by (†). Statistically significant differences (p-value<0.05) are indicated by an asterisk (*).</p

Functional outcomes in sham and experimental ischemia groups.

<p>A) Mean drop foot scores for all experimental groups. A drop foot score of 0 meant that no neuropathy was observed post-operatively. A drop foot score of 1 was consistent with neuropathy in the affected limb for at least one day post-operatively. A drop foot score of 2 was consistent with neuropathy in the affected limb for at least three days post-operatively. A drop foot score of 3 was indicative of neuropathy in the affected limb beyond 5 days post-operatively. B) Mean Tarlov scores for all experimental groups. The dashed line at 5.0 is reflective of normal locomotion. The asterisks indicate statistically significant differences when compared to sham and 3.5 hour occlusion animals—** = p-value < 0.01, *** = p-value < 0.001.</p

PLSDA models to predict return to normal locomotion for all data, imaging only data, and non-imaging data sets.

<p>Receiver operating curves for both calibration and cross-validation data sets. Area under the curve (AUC), a surrogate for accuracy, is displayed for each curve. A) PLSDA model generated from all parameters, but excluding outcome parameters. B) PLSDA model calculated from only imaging parameters. C) PLSDA model generated from all non-imaging parameters, but excluding outcome parameters. The calibration data is plotted in black and the cross-validation data is plotted in gray.</p

Slopes of reperfusion and post-occlusive reactive hyperemia for 3CCD and IR imaging.

<p>Bar plots comparing the IR slopes of reperfusion for all experimental groups (A) and 3CCD slopes of reperfusion, slopes of PORH at 5 minutes reperfusion, and slopes of PORH at 10 minutes reperfusion for all experimental groups (B). Statistically significant differences between 4.7 hour tourniquet animals and other experimental groups are designated by an asterisk (*)–p-value<0.05. Note, for each set of slopes presented in (B), the data is plotted by lowest Tarlov score (i.e. least normal locomotion) and increases to the highest Tarlov score (i.e. most normal locomotion). 3_5O = 3.5 hour occlusion; 3_5T = 3.5 hour tourniquet; 4_7O = 4.7 hour occlusion; 4_7T = 4.7 hour tourniquet.</p

Profiles of operative 3CCD and IR imaging values.

<p>Bar plots of mean R-B values derived from 3CCD imaging (A) and mean leg temperatures in °F (B) for all experimental groups at baseline, maximum ischemia, 10 minutes post-reperfusion, and 30 minutes post-reperfusion. Error bars = SEM (standard error of the mean). 3_5O = 3.5 hour occlusion; 3_5T = 3.5 hour tourniquet; 4_7O = 4.7 hour occlusion; 4_7T = 4.7 hour tourniquet.</p

Mean pathology scores for all experimental groups.

<p>Mean total pathology scores for all experimental groups: A) organs (kidneys and liver), and B) sciatic nerve and skeletal muscle. 3_5O = 3.5 hour occlusion; 3_5T = 3.5 hour tourniquet; 4_7O = 4.7 hour occlusion; 4_7T = 4.7 hour tourniquet.</p

Correlation of imaging parameters with blood chemistries.

<p>Pearson's correlation coefficients (ρ) for parameters involving blood chemistries. IR = infrared imaging; Rpf = Rpfusion; 3CCD = 3CCD imaging; PORH = post-occlusive reactive hyperemia; D = day.</p