5 research outputs found
Essential oil composition and antioxidant activity of two Juniperus communis L. varieties growing wild in Serbia
Leaf n-Alkanes as Characters Differentiating Coastal and Continental Juniperus deltoides Populations from the Balkan Peninsula
The composition of the cuticular n-alkanes isolated from the leaves of
nine populations of Juniperus deltoides R. P. Adams from continental and
coastal areas of the Balkan Peninsula was characterized by GC-FID and
GC/MS analyses. In the leaf waxes, 14 n-alkane homologues with
chain-lengths ranging from C22 to C35 were identified. n-Tritriacontane
(C33) was dominant in the waxes of all populations, but variations
between the populations in the contents of all n-alkanes were observed.
Several statistical methods (ANOVA, principal component, discriminant,
and cluster analyses) were used to investigate the diversity and
variability of the cuticular-leaf-n-alkane patterns of the nine J.
deltoides populations. This is the first report on the n-alkane
composition for this species. The multivariate statistical analyses
evidenced a high correlation of the leaf-n-alkane pattern with the
geographical distribution of the investigated samples, differentiating
the coastal from the continental populations of this taxon.Ministry of Education, Science and Technological Development of Serbia
{[}173029, 172053
Headspace volatiles isolated from twigs of Picea omorika from Serbia
The variability of volatiles isolated from twigs by the static headspace (HS) method in seven natural populations of Picea omorika from Serbia was investigated for the first time. In the overall chemical profile, monoterpenes strongly dominated hydrocarbons as the most volatile compounds (95.7%). The dominant compounds were α-pinene (29.5 %), β-pinene (25.7%) and myrcene (13.0%), totaling 68.2% of the volatiles on average. The following nine volatiles were found to be present in medium-to-high amounts (0.5-10%): tricyclene, camphene, α-phellandrene, δ-3-carene, p-cymene, β-phellandrene, terpinolene, (E)-caryophyllene, and germacrene D. Out of the 78 volatiles detected, the six most abundant (α-pinene, β-pinene, myrcene, δ-3-carene, β-phellandrene and camphene) were selected for principle component analysis (PCA) and cluster analyses (CA). PCA revealed a high degree of similarity between populations, while CA showed a degree of separation of two populations from the others. The presented results are in agreement with previous phytochemical and molecular analyses of this species that confirm high variability in both specialized metabolites and genetic markers
CROSS-SPECIES AMPLIFICATION OF NUCLEAR EST-MICROSATELLITES DEVELOPED FOR OTHER Pinus SPECIES IN Pinus nigra
Due to the current lack of nuclear microsatellites (simple sequence
repeats SSRs) specifically developed for Pinus nigra, an important
European coniferous species, we cross-species amplified 12 EST-SSRs
(expressed sequence tagged SSRs) developed for other Pinus species in P.
nigra in order to delineate loci which can be used for assessing levels
of genetic diversity and genetic structuring in this species. We
amplified these loci in individuals from seven populations from the
central Balkans representing four recognized infraspecific taxa of P.
nigra (ssp. nigra, var. gocensis, ssp. pallasiana, and var. banatica).
Contrary to expectations on high transferability of EST-SSRs into
related species, only three out of 12 tested loci were successfully
amplified in P. nigra, but they displayed lack/low levels of
polymorphism or generated multilocus amplification products. Thus, our
estimates on levels of genetic diversity (HE = 0.183) and genetic
differentiation (F-ST = 0.007) were based on variability of a single
locus harboring four alleles only and they should be taken with
cautions. Our study highlights the need for the development of
high-resolution molecular markers, such as co-dominant genic or genomic
SSRs or predominantly biallelic SNPs, or utilization of anonymous
dominant markers, such as AFLPs, for genotyping in P. nigra.Ministry of Education, Science, and Technology of the Republic of Serbia
{[}173029, 173030
Leaf n-Alkanes as Characters Differentiating Coastal and Continental Juniperus deltoides Populations from the Balkan Peninsula
The composition of the cuticular n-alkanes isolated from the leaves of
nine populations of Juniperus deltoides R. P. Adams from continental and
coastal areas of the Balkan Peninsula was characterized by GC-FID and
GC/MS analyses. In the leaf waxes, 14 n-alkane homologues with
chain-lengths ranging from C22 to C35 were identified. n-Tritriacontane
(C33) was dominant in the waxes of all populations, but variations
between the populations in the contents of all n-alkanes were observed.
Several statistical methods (ANOVA, principal component, discriminant,
and cluster analyses) were used to investigate the diversity and
variability of the cuticular-leaf-n-alkane patterns of the nine J.
deltoides populations. This is the first report on the n-alkane
composition for this species. The multivariate statistical analyses
evidenced a high correlation of the leaf-n-alkane pattern with the
geographical distribution of the investigated samples, differentiating
the coastal from the continental populations of this taxon.Ministry of Education, Science and Technological Development of Serbia
{[}173029, 172053