2 research outputs found
Elevated synaptic vesicle release probability in synaptophysin/gyrin family quadruple knockouts
Synaptophysins 1 and 2 and synaptogyrins 1 and 3 constitute a major family of
synaptic vesicle membrane proteins. Unlike other widely expressed synaptic vesicle proteins such
as vSNAREs and synaptotagmins, the primary function has not been resolved. Here, we report
robust elevation in the probability of release of readily releasable vesicles with both high and low
release probabilities at a variety of synapse types from knockout mice missing all four family
members. Neither the number of readily releasable vesicles, nor the timing of recruitment to the
readily releasable pool was affected. The results suggest that family members serve as negative
regulators of neurotransmission, acting directly at the level of exocytosis to dampen connection
strength selectively when presynaptic action potentials fire at low frequency. The widespread
expression suggests that chemical synapses may play a frequency filtering role in biological
computation that is more elemental than presently envisioned
Elevated synaptic vesicle release probability in synaptophysin/gyrin family quadruple knockouts
Synaptophysins 1 and 2 and synaptogyrins 1 and 3 constitute a major family of
synaptic vesicle membrane proteins. Unlike other widely expressed synaptic vesicle proteins such
as vSNAREs and synaptotagmins, the primary function has not been resolved. Here, we report
robust elevation in the probability of release of readily releasable vesicles with both high and low
release probabilities at a variety of synapse types from knockout mice missing all four family
members. Neither the number of readily releasable vesicles, nor the timing of recruitment to the
readily releasable pool was affected. The results suggest that family members serve as negative
regulators of neurotransmission, acting directly at the level of exocytosis to dampen connection
strength selectively when presynaptic action potentials fire at low frequency. The widespread
expression suggests that chemical synapses may play a frequency filtering role in biological
computation that is more elemental than presently envisioned