Regulation of miR394 in Response to Fusarium oxysporum f. sp. cepae (FOC) Infection in Garlic (Allium sativum L)

MicroRNAs (miRNAs) are a class of post transcriptional regulators that negatively regulate gene expression through target mRNA cleavage or translational inhibition and play important roles in plant development and stress response. In the present study, 6 conserved miRNAs from garlic (Allium sativum L.) were analysed to identify differentially expressed miRNAs in response to Fusarium oxysporum f. sp. cepae (FOC) infection. Stem-loop RT-PCR revealed that miR394 is significantly induced in garlic seedlings post treatment with FOC for 72 h. The induction of miR394 expression during FOC infection was restricted to the basal stem plate tissue, the primary site of infection. Garlic miR394 was also upregulated by exogenous application of jasmonic acid. Two putative targets of miR394 encoding F-box domain and cytochrome P450 (CYP450) family proteins were predicted and verified using 5’ RLM-RACE (RNA ligase mediated rapid amplification of cDNA ends) assay. Quantitative RT-PCR showed that the transcript levels of the predicted targets were significantly reduced in garlic plants exposed to FOC. When garlic cultivars with variable sensitivity to FOC were exposed to the pathogen, an upregulation of miR394 and down regulation of the targets were observed in both varieties. However, the expression pattern was delayed in the resistant genotypes. These results suggest that miR394 functions in negative modulation of FOC resistance and the difference in timing and levels of expression in variable genotypes could be examined as markers for selection of FOC resistant garlic cultivars

Histone modification enhances the effectiveness of IL-13 receptor targeted immunotoxin in murine models of human pancreatic cancer

<p>Abstract</p> <p>Background</p> <p>Interleukin-13 Receptor α2 (IL-13Rα2) is a tumor-associated antigen and target for cancer therapy. Since IL-13Rα2 is heterogeneously overexpressed in a variety of human cancers, it would be highly desirable to uniformly upregulate IL-13Rα2 expression in tumors for optimal targeting.</p> <p>Methods</p> <p>We examined epigenetic regulation of <it>IL-13Rα2 </it>in a murine model of human pancreatic cancer by Bisulfite-PCR, sequencing for DNA methylation and chromatin immunoprecipitation for histone modification. Reverse transcription-PCR was performed for examining changes in IL-13Rα2 mRNA expression after treatment with histone deacetylase (HDAC) and c-jun inhibitors. <it>In vitro </it>cytotoxicity assays and <it>in vivo </it>testing in animal tumor models were performed to determine whether HDAC inhibitors could enhance anti-tumor effects of IL-13-PE in pancreatic cancer. Mice harboring subcutaneous tumors were treated with HDAC inhibitors systemically and IL-13-PE intratumorally.</p> <p>Results</p> <p>We found that CpG sites in <it>IL-13Rα2 </it>promoter region were not methylated in all pancreatic cancer cell lines studied including IL-13Rα2-positive and IL-13Rα2-negative cell lines and normal cells. On the other hand, histones at IL-13Rα2 promoter region were highly-acetylated in IL-13Rα2-positive but much less in receptor-negative pancreatic cancer cell lines. When cells were treated with HDAC inhibitors, not only histone acetylation but also IL-13Rα2 expression was dramatically enhanced in receptor-negative pancreatic cancer cells. In contrast, HDAC inhibition did not increase IL-13Rα2 in normal cell lines. In addition, c-jun in IL-13Rα2-positive cells was expressed at higher level than in negative cells. Two types of c-jun inhibitors prevented increase of IL-13Rα2 by HDAC inhibitors. HDAC inhibitors dramatically sensitized cancer cells to immunotoxin in the cytotoxicity assay <it>in vitro </it>and increased IL-13Rα2 in the tumors subcutaneously implanted in the immunodeficient animals but not in normal mice tissues. Combination therapy with HDAC inhibitors and immunotoxin synergistically inhibited growth of not only IL-13Rα2-positive but also IL-13Rα2-negative tumors.</p> <p>Conclusions</p> <p>We have identified a novel function of histone modification in the regulation of IL-13Rα2 in pancreatic cancer cell lines <it>in vitro </it>and <it>in vivo</it>. HDAC inhibition provides a novel opportunity in designing combinatorial therapeutic approaches not only in combination with IL-13-PE but with other immunotoxins for therapy of pancreatic cancer and other cancers.</p

Near-IR studies of recurrent nova V745 Scorpii during its 2014 outburst

The recurrent nova (RN) V745 Scorpii underwent its third known outburst on 2014 February 6. Infrared monitoring of the eruption on an almost daily basis, starting from 1.3d after discovery, shows the emergence of a powerful blast wave generated by the high velocity nova ejecta exceeding 4000 kms$^{-1}$ plowing into its surrounding environment. The temperature of the shocked gas is raised to a high value exceeding 10$^{8}$K immediately after outburst commencement. The energetics of the outburst clearly surpass those of similar symbiotic systems like RS Oph and V407 Cyg which have giant secondaries. The shock does not show a free-expansion stage but rather shows a decelerative Sedov-Taylor phase from the beginning. Such strong shockfronts are known to be sites for $\gamma$ ray generation. V745 Sco is the latest nova, apart from five other known novae, to show $\gamma$ ray emission. It may be an important testbed to resolve the crucial question whether all novae are generically $\gamma$ ray emitters by virtue of having a circumbinary reservoir of material that is shocked by the ejecta rather than $\gamma$ ray generation being restricted to only symbiotic systems with a shocked red giant (RG) wind. The lack of a free-expansion stage favors V745 Sco to have a density enhancement around the white dwarf (WD), above that contributed by a RG wind. Our analysis also suggests that the WD in V745 Sco is very massive and a potential progenitor for a future SN Ia explosion.Comment: To appear in ApJ (Letters

Geostatistical based soil fertility mapping of Horticultural Research Station, Rajikot, Jumla, Nepal

Geostatistical soil mapping is important for determining spatial distribution of soil parameters. This study was conducted to assess soil spatial distribution of the Horticultural Research Station, Rajikot, Jumla, Nepal. The total 27 samples were collected randomly at a depth of 0-20 cm by using soil sampling auger. A GPS device was used for determination of geographical position of soil sampling points. The collected samples were analyzed following standard analytical methods in the laboratory of National Soil Science Research Centre, Khumaltar. The Arc-GIS 10.1 software was used for the mapping spatial distribution of various soil parameters. The observed data revealed the structure was sub-angular blocky and granular, whereas colour were dark brown to dark grayish brown and dark yellowish brown. The sand, silt and clay content were ranged 24.40-72.10%, 19.40-63.10% and 6.20-14.50%, respectively and categorized loam, sandy loam and silt loam in texture. The soil pH was slightly acidic to moderately alkaline (5.01-8.06), and very low available sulphur (0.59-2.41 mg/kg) and very low to low available boron (0.04-0.87 mg/kg).&nbsp; Moreover, very low to medium available manganese (2.18-13.46 mg/kg), very low to very high available iron (4.50-138.58 mg/kg), and low to medium available magnesium (7.20-177.60.53 mg/kg) and zinc (0.26-1.66 mg/kg). Similarly, medium total nitrogen (0.09-0.22%), medium to high organic matter (1.71-6.26%) and available calcium (1200-3144 mg/kg), medium to very high available phosphorus (3.71-82.4 mg/kg) and potassium (59.37-173.05 mg/kg). Correspondingly, high to very high available copper (0.78-4.20 mg/kg). The determined soil test data can be used for sustainable soil management as well as developing future research strategy in the farm

Higher education and participation of indigenous people in India: some reflections

Indigenous people are the most impoverished and socially disadvantaged groups in India. They account for 8.2% of the total Indian population. This group stands at the lower strata of access and participation in higher education despite constitutional protection and strong Government affirmative action policies. The Gross Enrolment Ratio (GER) of these groups has improved significantly during the last decade, but the participation in absolute terms is much below the national average. This paper examines issues concerning higher education access and equity for communities of Indigenous people, gender disparity within this group and also highlights their unique problems which may require divergent policy responses.Etninių grupių atstovai yra labiausiai nuskurdusi ir socialiai diskriminuojama grupė Indijoje. Jie sudaro apie 8,2 proc. visų Indijos gyventojų. Nepaisant Konstitucijoje numatytos paramos ir gausių Vyriausybės skatinamųjų priemonių, ši grupė nepakyla nuo žemiausios pakopos siekdama aukštojo mokslo. Bendra tokių etninių grupių įstojusiųjų asmenų proporcija (The Gross Enrolment Ratio, GER) per pastaruosius dešimt metų ženkliai išaugo, bet absoliutus skaičius rodo žymų atotrūkį nuo nacionalinio vidurkio. Šiame straipsnyje aptariamas aukštojo mokslo prieinamumas ir etninių grupių bendruomenių lygiateisiškumas, lyčių santykis šiose grupėse, pabrėžiamas problemų išskirtinumas ir tai, jog šios problemos reikalauja įvirialypių politinių sprendimų

Zika Virus- Emergence, Evolution, Pathology, Diagnosis, and Control: Current Global Scenario and Future Perspectives- A Comprehensive Review

This review converses the Zika virus which has attained global concern due to its rapid pandemic potential and impact on humans. Though Zika virus was first isolated in 1947, till the recent large-scale outbreak which occurred in Micronesia, in 2007, the virus was placed into the innocuous pathogen category. The World Health Organization on 1 February 2016 declared it as a Public Health Emergency of International Concern.\u27 Of the note, American as well as Pacific Island strains/isolates is relatively closer to Asian lineage strains. The African and American strains share more than 87.5% and 95% homologies with Asian strains/isolates, respectively. Asian strains form independent clusters, except those isolated from China, suggesting relatively more diversity than African strains. Prevention and control are mainly aimed at the vector population (mosquitoes) with Aedes aegypti being the main species. Surveys in Africa and Asia indicated seropositivity in various animal species. However, so far its natural reservoir is unknown. There is an urgent need to understand why Zika virus has shifted from being a virus that caused mild illness to unforeseen birth defects as well as autoimmune-neurological problems. Unfortunately, an effective vaccine is not available yet. Availability of cryo-electron microscopy based on 3.8 angstrom resolution revealing mature Zika virus structure and the probable virus attachment site to host cell would provide critical insights into the development of antiviral treatments and vaccines

Rheological Behavior of Aqueous Suspensions of Laponite: New Insights into the Ageing Phenomena

In this paper, ageing behavior of suspensions of laponite with varying salt concentration is investigated using rheological tools. It is observed that the ageing is accompanied by an increase in the complex viscosity. The succeeding creep experiments performed at various ages showed damped oscillations in the strain. The characteristic time-scale of the damped oscillations, retardation time, showed a prominent decrease with the age of the system. However, this dependence weakens with an increase in the salt concentration, which is known to change microstructure of the system from glass-like to gel-like. We postulate that a decrease in the retardation time can be represented as a decrease in the viscosity (friction) of the dissipative environment surrounding the arrested entities that opposes elastic deformation of the system. We believe that ageing in colloidal glass leads to a greater ordering that enhances relative spacing between the constituents thereby reducing the frictional resistance. However, since a gel state is inherently different in structure (fractal network) than that of a glass (disordered), ageing in the same does not induce ordering. Consequently, we observe inverse dependence of retardation time on age becoming weaker with an increase in the salt concentration. We analyze these results from a perspective of ageing dynamics of both glass state and gel state of laponite suspensions.Comment: 27 Pages, 12 figure