Establishement of plasma membrane politary in mammary epithelial cells correlates with changes in prolactin trafficking and in annexin VI recruitment to membranes

International audienc

Lipidomics of the red cell in diagnosis of human disorders.

International audienceApplications of tandem mass spectrometry in the field of lipid clinical chemistry are considered. Haemato-logical and biochemical advantages are presented favoring the choice of red blood cell membranes as a starting material in a wide variety of biomedical fields. Practical considerations are discussed with respect to methods of sampling, storage, and lipid extraction of red blood cells. The chapter describes the capabilities of a direct infusion of raw lipid extracts in the electro-spray ionization source compared with the more sophisticated method of high-performance liquid chromatography coupled with hybrid tandem mass spectrometry. Both methods have been evaluated and have been shown to be suitable for diagnosis and/or monitoring for a variety of human disorders