Causes and biophysical consequences of cellulose production by Pseudomonas fluorescens SBW25 at the air-liquid interface

Cellulose over-producing wrinkly spreader mutants of Pseudomonas fluorescens SBW25 have been the focus of much investigation, but conditions promoting the production of cellulose in ancestral SBW25, its effects and consequences have escaped in-depth investigation through lack of in vitro phenotype. Here, using a custom built device, we reveal that in static broth microcosms ancestral SBW25 encounters environmental signals at the air-liquid interface that activate, via three diguanylate cyclase-encoding pathways (Wsp, Aws and Mws), production of cellulose. Secretion of the polymer at the meniscus leads to modification of the environment and growth of numerous micro-colonies that extend from the surface. Accumulation of cellulose and associated microbial growth leads to Rayleigh-Taylor instability resulting in bioconvection and rapid transport of water-soluble products over tens of millimetres. Drawing upon data we build a mathematical model that recapitulates experimental results and captures the interactions between biological, chemical and physical processes.IMPORTANCE This work reveals a hitherto unrecognized behaviour that manifests at the air-liquid interface, which depends on production of cellulose, and hints to undiscovered dimensions to bacterial life at surfaces. Additionally, the study links activation of known diguanylate cyclase-encoding pathways to cellulose expression and to signals encountered at the meniscus. Further significance stems from recognition of the consequences of fluid instabilities arising from surface production of cellulose for transport of water-soluble products over large distances

Understanding the Wolbachia-mediated inhibition of arboviruses in mosquitoes: progress and challenges

Arthropod-borne viruses (arboviruses) pose a considerable threat to human and animal health, yet effective control measures have proven difficult to implement, and novel means of controlling their replication in arthropod vectors, such as mosquitoes, are urgently required. One of the most exciting approaches to emerge from research on arthropods is the use of the endosymbiotic intracellular bacterium Wolbachia to control arbovirus transmission from mosquito to vertebrate. These α-proteobacteria propagate through insects, in part through modulation of host reproduction, thus ensuring spread through species and maintenance in nature. Since it was discovered that Wolbachia endosymbiosis inhibits insect virus replication in Drosophila species, these bacteria have also been shown to inhibit arbovirus replication and spread in mosquitoes. Importantly, it is not clear how these antiviral effects are mediated. This review will summarize recent work and discuss determinants of antiviral effectiveness that may differ between individual Wolbachia/vector/arbovirus interactions. We will also discuss the application of this approach to field settings and the associated risks

Social Control Techniques in College Athletics: An Application of Goffman and Foucault

College athletics originated as a recess from academic demands and were originally organized by students, yet faculty took over supervision to address safety, professionalism, and academic integrity (Hawkins, 2010). This evolution led to the formation of athletic departments and a governing body (Shulman & Bowen, 2001; Hawkins, 2010). Thus, demonstrating a historical element of social control in the motives of sporting establishments; however, official aims such as character building are typically espoused (Shulman & Bowen, 2001), or as Goffman (1961) states, “the reformation of inmates in the direction of some ideal standard” (p. 74). Examination of the use of available resources within NCAA Division I institutions raises questions concerning relationships of power, locus of control, and the mission of universities and athletic departments, (Benford, 2007; Hughes & Coakley, 1991; Shulman & Bowen, 2001). This study analyzes how social control techniques have manifested within NCAA Division I intercollegiate athletics as a perpetuation of commercialization. The experience of three NCAA Division I softball student-athletes is presented. The work is methodologically grounded in the theoretical paradigm of pragmatism and is informed substantively by Foucault’s (1979/1995) panopticism and Goffman’s (1961) total institution. A narrative inquiry as defined by Clandidin and Connelly (1994; 2001; 2006) was designed using semi-structured interviews for data collection. The participants’ unique stories illustrate how they experience the disciplines of a total institution. A polycoval method of data analysis was used to ensure that the multiple voices present in the data were represented (Hatch, 2002). This study expands understanding of how the issues of power, social control, and personal empowerment are experienced by NCAA Division I softball student-athletes

A submillimetre wavelength spectral line search of the Orion molecular cloud core

A submillimetre wavelength molecular line search of the Orion molecular cloud has been made covering a total of about 5 percent of the frequency range 342.8 - 358.6 GHz. This search, coupled with the authors' previous observations of submillimetre transitions in this cloud, has led to the detection of 22 transitions of 14 molecular species, of which 16 are reported here for the first time. No unidentified lines have been detected in the present search. Mapping observations have been obtained for several of the lines and, in the case of H2CO the authors have been able to compare the present data with that obtained from other telescopes, to estimate the density and abundance in the emitting region

How Well Can We Predict Wildlife Corridors? Tests of Alternative Modeling Approaches in Migratory Elk and Dispersing Wolverines

Landscape connectivity has become a key focus of conservation biology as natural habitat is increasingly fragmented by human land use. Several landscape modeling approaches are now relied upon to identify likely dispersal and migration corridors and guide conservation planning. However, the predictive accuracy of these methods has seen limited testing against empirical movement data, which limits confidence in their utility and confuses selection of appropriate methods for a given application. To address these issues, we used GPS collar data from migrating elk and dispersing wolverines to evaluate the ability of common modeling approaches (cost-distance/least-cost path models and circuit theory models) to predict observed movement routes. While both methods made generally similar predictions, cost-distance models consistently outperformed circuit theory models, and predictive success was much higher for elk than for wolverine movements. Furthermore, the form and complexity of underlying landscape resistance maps influenced model performance and revealed unforeseen differences between models. These findings illustrate that corridor model performance depends greatly on focal species and landscape characteristics as well as selection of appropriate methods for the application at hand

Impact of imputation methods on the amount of genetic variation captured by a single-nucleotide polymorphism panel in soybeans

Background Success in genome-wide association studies and marker-assisted selection depends on good phenotypic and genotypic data. The more complete this data is, the more powerful will be the results of analysis. Nevertheless, there are next-generation technologies that seek to provide genotypic information in spite of great proportions of missing data. The procedures these technologies use to impute genetic data, therefore, greatly affect downstream analyses. This study aims to (1) compare the genetic variance in a single-nucleotide polymorphism panel of soybean with missing data imputed using various methods, (2) evaluate the imputation accuracy and post-imputation quality associated with these methods, and (3) evaluate the impact of imputation method on heritability and the accuracy of genome-wide prediction of soybean traits. The imputation methods we evaluated were as follows: multivariate mixed model, hidden Markov model, logical algorithm, k-nearest neighbor, single value decomposition, and random forest. We used raw genotypes from the SoyNAM project and the following phenotypes: plant height, days to maturity, grain yield, and seed protein composition. Results We propose an imputation method based on multivariate mixed models using pedigree information. Our methods comparison indicate that heritability of traits can be affected by the imputation method. Genotypes with missing values imputed with methods that make use of genealogic information can favor genetic analysis of highly polygenic traits, but not genome-wide prediction accuracy. The genotypic matrix captured the highest amount of genetic variance when missing loci were imputed by the method proposed in this paper. Conclusions We concluded that hidden Markov models and random forest imputation are more suitable to studies that aim analyses of highly heritable traits while pedigree-based methods can be used to best analyze traits with low heritability. Despite the notable contribution to heritability, advantages in genomic prediction were not observed by changing the imputation method. We identified significant differences across imputation methods in a dataset missing 20 % of the genotypic values. It means that genotypic data from genotyping technologies that provide a high proportion of missing values, such as GBS, should be handled carefully because the imputation method will impact downstream analysis

In vivo transcriptome analysis provides insights into host-dependent expression of virulence factors by Yersinia entomophaga MH96, during infection of Galleria mellonella

The function of microbes can be inferred from knowledge of genes specifically expressed in natural environments. Here, we report the in vivo transcriptome of the entomopathogenic bacterium Yersinia entomophaga MH96, captured during initial, septicemic, and pre-cadaveric stages of intrahemocoelic infection in Galleria mellonella. A total of 1285 genes were significantly upregulated by MH96 during infection; 829 genes responded to in vivo conditions during at least one stage of infection, 289 responded during two stages of infection, and 167 transcripts responded throughout all three stages of infection compared to in vitro conditions at equivalent cell densities. Genes upregulated during the earliest infection stage included components of the insecticidal toxin complex Yen-TC (chi1, chi2, and yenC1), genes for rearrangement hotspot element containing protein yenC3, cytolethal distending toxin cdtAB, and vegetative insecticidal toxin vip2. Genes more highly expressed throughout the infection cycle included the putative heat-stable enterotoxin yenT and three adhesins (usher-chaperone fimbria, filamentous hemagglutinin, and an AidA-like secreted adhesin). Clustering and functional enrichment of gene expression data also revealed expression of genes encoding type III and VI secretion system-associated effectors. Together these data provide insight into the pathobiology of MH96 and serve as an important resource supporting efforts to identify novel insecticidal agents

The cell adhesion molecule Fasciclin2 regulates brush border length and organization in Drosophila renal tubules

Multicellular organisms rely on cell adhesion molecules to coordinate cell–cell interactions, and to provide navigational cues during tissue formation. In Drosophila, Fasciclin 2 (Fas2) has been intensively studied due to its role in nervous system development and maintenance; yet, Fas2 is most abundantly expressed in the adult renal (Malpighian) tubule rather than in neuronal tissues. The role Fas2 serves in this epithelium is unknown. Here we show that Fas2 is essential to brush border maintenance in renal tubules of Drosophila. Fas2 is dynamically expressed during tubule morphogenesis, localizing to the brush border whenever the tissue is transport competent. Genetic manipulations of Fas2 expression levels impact on both microvilli length and organization, which in turn dramatically affect stimulated rates of fluid secretion by the tissue. Consequently, we demonstrate a radically different role for this well-known cell adhesion molecule, and propose that Fas2-mediated intermicrovillar homophilic adhesion complexes help stabilize the brush border