1 research outputs found
Protein Interactions with Nanoengineered Polyoxazoline Surfaces Generated via Plasma Deposition
Protein adsorption
to biomaterials is critical in determining their
suitability for specific applications, such as implants or biosensors.
Here, we show that surface nanoroughness can be tailored to control
the covalent binding of proteins to plasma-deposited polyoxazoline
(PPOx). Nanoengineered surfaces were created by immobilizing gold
nanoparticles varying in size and surface density on PPOx films. To
keep the surface chemistry consistent while preserving the nanotopography,
all substrates were overcoated with a nanothin PPOx film. Bovine serum
albumin was chosen to study protein interactions with the nanoengineered
surfaces. The results demonstrate that the amount of protein bound
to the surface is not directly correlated with the increase in surface
area. Instead, it is determined by nanotopography-induced geometric
effects and surface wettability. A densely packed array of 16 and
38 nm nanoparticles hinders protein adsorption compared to smooth
PPOx substrates, while it increases for 68 nm nanoparticles. These
adaptable surfaces could be used for designing biomaterials where
proteins adsorption is or is not desirable