Characterization of Dynamic Regulatory Gene and Protein Networks in Wheat Roots Upon Perceiving Water Deficit Through Comparative Transcriptomics Survey

A well-developed root system benefits host plants by optimizing water absorption and nutrient uptake and thereby increases plant productivity. In this study we have characterized the root transcriptome using RNA-seq and subsequential functional analysis in a set of drought tolerant and susceptible genotypes. The goal of the study was to elucidate and characterize water deficit-responsive genes in wheat landraces that had been through long-term field and biochemical screening for drought tolerance. The results confirm genotype differences in water-deficit tolerance in line with earlier results from field trials. The transcriptomics survey highlighted a total of 14,187 differentially expressed genes (DEGs) that responded to water deficit. The characterization of these genes shows that all chromosomes contribute to water-deficit tolerance, but to different degrees, and the B genome showed higher involvement than the A and D genomes. The DEGs were mainly mapped to flavonoid, phenylpropanoid, and diterpenoid biosynthesis pathways, as well as glutathione metabolism and hormone signaling. Furthermore, extracellular region, apoplast, cell periphery, and external encapsulating structure were the main water deficit-responsive cellular components in roots. A total of 1,377 DEGs were also predicted to function as transcription factors (TFs) from different families regulating downstream cascades. TFs from the AP2/ERF-ERF, MYB-related, B3, WRKY, Tify, and NAC families were the main genotype-specific regulatory factors. To further characterize the dynamic biosynthetic pathways, protein-protein interaction (PPI) networks were constructed using significant KEGG proteins and putative TFs. In PPIs, enzymes from the CYP450, TaABA8OH2, PAL, and GST families play important roles in water-deficit tolerance in connection with MYB13-1, MADS-box, and NAC transcription factors

BVRI photometric observations, light curve solutions and orbital period analysis of BF Pav

A new ephemeris, period change analysis and light curve modeling of the W UMa-type eclipsing binary BF Pav are presented in this study. Light curves of the system taken in BVRI filters from two observatories, in Australia and Argentina, were modeled using the Wilson-Devinney code. The results of this analysis demonstrate that BF Pav is a contact binary system with a photometric mass ratio q = 1.460 ± 0.014, a fillout factor f = 12.5%, an inclination of 87.97 ± 0.45 deg and a cold spot on the secondary component. By applying the distance modulus formula, the distance of BF Pav was calculated to be d = 268 ± 18 pc which is in good agreement with the Gaia EDR3 distance. We obtain an orbital period increase at a rate of 0.142 s century−1 due to a quadratic trend in the O − C diagram. Also, an alternative sudden period jump probably occurred which could be interpreted as a rapid mass transfer from the lower mass star to its companion of about ∆M = 2.45×10−6 M. Furthermore, there is an oscillatory behavior with a period of 18.3 ± 0.3 yr. Since BF Pav does not seem to have significant magnetic activity, this behavior could be interpreted as the light-time effect caused by an undetected third body in this system. In this case, the probability for the third body to be a low mass star with M ≥ 0.075 M or a brown dwarf is 5.4% and 94.6% respectively. If we assume i0 = 90◦, a3 = 8.04 ± 0.33 AU. The mass of the secondary component was also determined following two different methods which result close to each other.Fil: Poro, Atila. The International Occultation Timing Association Middle East section; IránFil: Alicavus, Fahri. Canakkale Onsekiz Mart University; TurquíaFil: Fernandez Lajus, Eduardo Eusebio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Astrofísica La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Astronómicas y Geofísicas. Instituto de Astrofísica La Plata; ArgentinaFil: Davoudi, Fatemeh. The International Occultation Timing Association Middle East section; IránFil: MirshafieKhozani, PegahSadat. The International Occultation Timing Association Middle East section; IránFil: Blackford, Mark G.. Congarinni Observatory; AustraliaFil: Budding, Edwin. Université du Québec a Montreal; CanadáFil: Jalalabadi, Behjat Zarei. Carter Observatory; Nueva ZelandaFil: Rahimi, Jabar. The International Occultation Timing Association Middle; IránFil: Farahani, Farzaneh Ahangarani. The International Occultation Timing Association Middle; Irá