Full-Body Circuit Training Improves Body Composition and Cardiorespiratory Fitness in Overweight Sedentary Adults – A Randomized Controlled Trial

Obesity and low cardiorespiratory fitness are major risk factors for numerous non-communicable diseases and mortality, and efficient treatment protocols to counteract these conditions are highly warranted. We evaluated the effect of high-intensity circuit training (CIT) on body composition and cardiorespiratory fitness of sedentary overweight individuals. Cardiorespiratory and body composition were assessed before and after a 8-week circuit training (CIT; four sessions of full-body CIT per week; age, 38 ± 9 years old; height, 174 ± 10 cm; body mass, 93.1 ± 22.2 kg; n = 32), and 8-week inactiv-ity (CON; neither training nor lifestyle changes from week; 0–8 age, 39 ± 7 years old, height, 168 ± 8 cm, body mass, 89.5 ± 17.5 kg; n = 33). The two-way repeated-measures ANOVA revealed moderate to large reductions were observed in body mass, BMI, and fat percentage after CIT (d=0.43–0.81; P<0.05). In contrast, all body composition parameters remained stable after 8-week inactivity (P<0.05). A small to moderate group x moment interaction was found for body mass, BMI and % fat (d=0.10–0.60; P<0.05). Small improvements in VO2max were observed after CIT (d=0.48 [0.11–0.85]; P=0.010), while small to moderate reductions were observed in VO2max and PV were observed after inactivity (d=0.47 [0.11–0.83] and 0.64 [0.26–1.01], respectively; P<0.05). A small moment x group interaction was observed for VO2max (d= 0.19 [0.13–0.26]; P<0.001). Our findings suggest that 8-week of full-body circuit training may improve cardiorespiratory fitness and body composition in sedentary overweight individuals

Application of Individualized Speed Zones to Quantify External Training Load in Professional Soccer

This study aimed to examine the interchangeability of two external training load (ETL) monitoring methods: arbitrary vs. individualized speed zones. Thirteen male outfield players from a professional soccer team were monitored during training sessions using 10-Hz GPS units over an 8-week competitive period (n = 302 observations). Low-speed activities (LSA), moderate-speed running (MSR), high-speed running (HSR) and sprinting were defined using arbitrary speed zones as <14.4, 14.4-19.8, 19.8-25.1 and ≥25.2 km·h-1, and using individualized speed zones based on a combination of maximal aerobic speed (MAS, derived from the Yo-yo Intermittent recovery test level 1), maximal sprinting speed (MSS, derived from the maximal speed reached during training) and anaerobic speed reserve (ASR) as <80% MAS, 80-100% MAS, 100% MAS or 29% ASR and ≥30% ASR. Distance covered in both arbitrary and individualized methods was almost certainly correlated in all speed zones (p < 0.01; r = 0.67-0.78). However, significant differences between methods were observed in all speed zones (p < 0.01). LSA was almost certainly higher when using the arbitrary method than when using the individualized method (p < 0.01; ES = 5.47 [5.18; 5.76], respectively). Conversely, MSR, HSR and sprinting speed were higher in the individualized method than in the arbitrary method (p < 0.01; ES = 5.10 [4.82; 5.37], 0.86 [0.72; 1.00] and 1.22 [1.08; 1.37], respectively). Arbitrary and individualized methods for ETL quantification based on speed zones showed similar sensitivity in depicting player locomotor demands. However, since these methods significantly differ at absolute level (based on measurement bias), arbitrary and individualized speed zones should not be used interchangeably.info:eu-repo/semantics/publishedVersio

Intra-individual variability of sleep and nocturnal cardiac autonomic activity in elite female soccer players during an international tournament

To describe individual sleeping patterns and nocturnal cardiac autonomic activity of National team female soccer players during an international tournament.info:eu-repo/semantics/publishedVersio

Anthropometric and functional profile of selected vs. non-selected 13-to-17-year-old soccer players

The purpose of this study was to compare anthropometric and functional profiles of 13-to-17-year-old soccer players according to their competitive level. Height, body mass, percentage of body fat, countermovement jump height, change of direction ability, 5- and 15-m sprint times, repeated sprint ability (RSA), intermittent recovery performance, and dribbling skills were collected in 115 young Italian soccer players. Players were divided into selected (i.e., competing at national level, n = 17 U15 and 47 U17) and non-selected (i.e., competing at regional level, n = 43 U15 and 8 U17) groups. U17 selected players were taller, quicker over 5 and 15 m, more agile, and had better RSA, prolonged intermittent recovery ability, and dribbling skills than their non-selected counterparts (d = 0.28-0.55, p &lt; 0.05). In particular, selected players showed lower times on the first three and the last shuttle of the RSA test (d = 0.28-0.34, p &lt; 0.05). No significant differences were observed in U15 players (p &gt; 0.05). Discriminant analysis revealed that dribbling skills, 15-m sprint time, and height best discriminate U17 players by competitive level (p &lt; 0.001). Anthropometric characteristics and functional abilities can discriminate across competitive standards between male U17 but not U15 soccer players. In particular, these findings suggest the importance of dribbling skills, 15-m sprint, and height in U17 players

Cholesterol and Its Metabolites in Tumor Growth: Therapeutic Potential of Statins in Cancer Treatment

Cholesterol is essential for cell function and viability. It is a component of the plasma membrane and lipid rafts and is a precursor for bile acids, steroid hormones, and Vitamin D. As a ligand for estrogen-related receptor alpha (ESRRA), cholesterol becomes a signaling molecule. Furthermore, cholesterol-derived oxysterols activate liver X receptors (LXRs) or estrogen receptors (ERs). Several studies performed in cancer cells reveal that cholesterol synthesis is enhanced compared to normal cells. Additionally, high serum cholesterol levels are associated with increased risk for many cancers, but thus far, clinical trials with 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors (statins) have had mixed results. Statins inhibit cholesterol synthesis within cells through the inhibition of HMG-CoA reductase, the rate-limiting enzyme in the mevalonate and cholesterol synthetic pathway. Many downstream products of mevalonate have a role in cell proliferation, since they are required for maintenance of membrane integrity; signaling, as some proteins to be active must undergo prenylation; protein synthesis, as isopentenyladenine is an essential substrate for the modification of certain tRNAs; and cell-cycle progression. In this review starting from recent acquired findings on the role that cholesterol and its metabolites fulfill in the contest of cancer cells, we discuss the results of studies focused to investigate the use of statins in order to prevent cancer growth and metastasis

Cholesterol as an Endogenous ERRα Agonist: A New Perspective to Cancer Treatment

The estrogen-related receptors (ERRs) are important members of nuclear receptors which contain three isoforms (α, β, and γ). ERRα is the best-characterized isoform expressed mainly in high-energy demanding tissues where it preferentially works in association with the peroxisome proliferator-activated receptor-γ co-activator 1α (PGC-1α) and PGC-1β. ERRα together with its cofactors modulates cellular metabolism, supports the growth of rapidly dividing cells, directs metabolic programs required for cell differentiation and maintains cellular energy homeostasis in differentiated cells. In cancer cells, the functional association between ERRα and PGC-1s is further influenced by oncogenic signals and induces metabolic programs favoring cell growth and proliferation as well as tumor progression. Recently, cholesterol has been identified as a natural ERRα ligand using a combined biochemical strategy. This new finding highlighted some important physiological aspects related to the use of cholesterol-lowering drugs such as statins and bisphosphonates. Even more meaningful is the link between increased cholesterol levels and certain cancer phenotypes characterized by an overexpressed ERRα such as mammary, prostatic, and colorectal cancers, where the metabolic adaptation affects many cancer processes. Moreover, high-energy demanding cancer-related processes are strictly related to the cross-talk between tumor cells and some key players of tumor microenvironment, such as tumor-associated macrophage that fuels cancer progression. Some evidence suggests that high cholesterol content and ERRα activity favor the inflammatory environment by the production of different cytokines. In this review, starting from the most recent observations on the physiological role of the new signaling activated by the natural ligand of ERRα, we propose a new hypothesis on the suitability to control cholesterol levels as a chance in modulating ERRα activity in those tumors in which its expression and activity are increased

Training in women soccer players: A systematic review on training load monitoring

ObjectiveThe present systematic review aimed to provide an overview of training load (TL), along with their responses, monitoring during training sessions in highly trained and elite adult women soccer players.Data sourceElectronic databases searches (PubMed, Scopus, Web of Science and Ebsco) for relevant studies published in peer-reviewed journals were conducted, and eligibility criteria were based on the PICOS model in accordance with PRISMA guidelines.Study selectionStudies were considered as follows: (a) highly trained and elite adult (&gt;18 years) women’s soccer players; (b) continuous (minimum 1-week duration) TL monitoring in the context of the team routine; (c) TL collected from entire training session. Methodological qualitative assessments and risk of bias criteria were used for judging the studies.Data extractionA total of 1,163 studies were identified, and 16 were included. The selected studies were fully screened to extract the population characteristics; the number of players; a type of study design; region where the study was performed; the main findings.Data synthesisAccumulated external TL (ETL) during the pre-season was positively correlated to enhanced adaptations in intermittent exercise capacity. Daily ETL was negatively correlated to next-day self-reported fatigue and muscle soreness. Daily internal TL (ITL) was negatively correlated to post-session sleep duration and sleep efficiency. One study showed that higher accumulated player load and total distance were associated with injury.ConclusionInformation about TL during training sessions in women soccer players is very sparse, and it is currently very difficult to consider evidence-based practices for training sessions in highly trained and elite adult women soccer players. Moreover, the dose–response relationships between TL and training outcome (e.g., fatigue, training adaptations and injuries) need to be further explored to understand the optimal training stimulus to enhance performance outcomes while preserving player health

Statins reduce intratumor cholesterol affecting adrenocortical cancer growth

Mitotane causes hypercholesterolemia in ACC patients. We suppose that cholesterol increases within the tumor and can be used to activate proliferative pathways. In this study, we used statins to decrease intratumor cholesterol and investigated the effects on ACC growth related to ER\u3b1 action at the nuclear and mitochondrial levels. We first used microarray to investigate mitotane effect on genes involved in cholesterol homeostasis and evaluated their relationship with patients' survival in ACC TCGA. We then blocked cholesterol synthesis with simvastatin and determined the effects on H295R cell proliferation, estradiol production and ER\u3b1 activity in vitro and in xenograft tumors. We found that mitotane increases intratumor cholesterol content and expression of genes involved in cholesterol homeostasis, among them INSIG, whose expression affects patients' survival. Treatment of H295R cells with simvastatin to block cholesterol synthesis decreased cellular cholesterol content and this affected cell viability. Simvastatin reduced estradiol production and decreased nuclear and mitochondrial ER\u3b1 function. A mitochondrial target of ER\u3b1, the respiratory complex IV (COX IV) was reduced after simvastatin treatment, which profoundly affected mitochondrial respiration activating apoptosis. In vivo experiments confirmed the ability of simvastatin to reduce tumor volume and weight of grafted H295R cells, intratumor cholesterol content, Ki-67 and ER\u3b1, COX IV expression and activity and increase TUNEL positive cells. Collectively these data demonstrate that a reduction in intratumor cholesterol content prevents estradiol production, inhibits mitochondrial respiratory chain inducing apoptosis in ACC cells. Inhibition of mitochondrial respiration by simvastatin represents a novel strategy to counteract ACC growth