Structural relationships between the NADH dehydrogenases of Paracoccus denitrificans and bovine heart mitochondria as revealed by immunological cross-reactivities

AbstractAn antibody raised against two subunits (Mr, 48000 and 25000) of NADH dehydrogenase from Paracoccusdenitrificans cross-reacts with one of more than 20 polypeptides that form the bovine heart mitochondrial NADH dehydrogenase. The cross-reacting subunit has Mr, 51000 and is believed to be the NADH-binding subunit of the enzyme. Antibodies raised against certain subunits of the bovine heart NADH dehydrogenase were tested for cross-reactivity withP. denitrificans cytoplasmic membranes. Of those tested, only one, an antibody specific for the 49 kDa subunit of mitochondrial NADH dehydrogenase, cross-reacted with the bacterial membranes. It recognised a polypeptide of approximate Mr, 46000. This is an indication for a previously undetected third subunit of NADH dehydrogenase from P. denitrificans. The immunological crossreactions indicate that the NADH dehydrogenases from P. denitrificans and bovine heart mitochondria are related structurally

Molecular Line Observations of Infrared Dark Clouds: Seeking the Precursors to Intermediate and Massive Star Formation

We have identified 41 infrared dark clouds from the 8 micron maps of the Midcourse Space Experiment (MSX), selected to be found within one square degree areas centered on known ultracompact HII regions. We have mapped these infrared dark clouds in N2H+(1-0), CS(2-1) and C18O(1-0) emission using the Five College Radio Astronomy Observatory. The maps of the different species often show striking differences in morphologies, indicating differences in evolutionary state and/or the presence of undetected, deeply embedded protostars. We derive an average mass for these clouds using N2H+ column densities of ~2500 solar masses, a value comparable to that found in previous studies of high mass star forming cores using other mass tracers. The linewidths of these clouds are typically ~2.0 - 2.9 km/s. Based on the fact that they are dark at 8 micron, compact, massive, and have large velocity dispersions, we suggest that these clouds may be the precursor sites of intermediate and high mass star formation.Comment: Accepted to ApJS, 22 pages, 10 pages of figures. For full-resolution images, see http://www.astro.lsa.umich.edu/~seragan/pubs/fcrao/figures.tar.g

NEATH II: N2_2H+^+ as a tracer of imminent star formation in quiescent high-density gas

Star formation activity in molecular clouds is often found to be correlated with the amount of material above a column density threshold of $\sim 10^{22} \, {\rm cm^{-2}}$. Attempts to connect this column density threshold to a ${\it volume}$ density above which star formation can occur are limited by the fact that the volume density of gas is difficult to reliably measure from observations. We post-process hydrodynamical simulations of molecular clouds with a time-dependent chemical network, and investigate the connection between commonly-observed molecular species and star formation activity. We find that many molecules widely assumed to specifically trace the dense, star-forming component of molecular clouds (e.g. HCN, HCO$^+$, CS) actually also exist in substantial quantities in material only transiently enhanced in density, which will eventually return to a more diffuse state without forming any stars. By contrast, N$_2$H$^+$ only exists in detectable quantities above a volume density of $10^4 \, {\rm cm^{-3}}$, the point at which CO, which reacts destructively with N$_2$H$^+$, begins to deplete out of the gas phase onto grain surfaces. This density threshold for detectable quantities of N$_2$H$^+$ corresponds very closely to the volume density at which gas becomes irreversibly gravitationally bound in the simulations: the material traced by N$_2$H$^+$ never reverts to lower densities, and quiescent regions of molecular clouds with visible N$_2$H$^+$ emission are destined to eventually form stars. The N$_2$H$^+$ line intensity is likely to directly correlate with the star formation rate averaged over timescales of around a Myr.Comment: 10 pages, 10 figures. MNRAS accepte

Non-Equilibrium Abundances Treated Holistically (NEATH): the molecular composition of star-forming clouds

Much of what we know about molecular clouds, and by extension star formation, comes from molecular line observations. Interpreting these correctly requires knowledge of the underlying molecular abundances. Simulations of molecular clouds typically only model species that are important for the gas thermodynamics, which tend to be poor tracers of the denser material where stars form. We construct a framework for post-processing these simulations with a full time-dependent chemical network, allowing us to model the behaviour of observationally-important species not present in the reduced network used for the thermodynamics. We use this to investigate the chemical evolution of molecular gas under realistic physical conditions. We find that molecules can be divided into those which reach peak abundances at moderate densities ($10^3 \, {\rm cm^{-3}}$) and decline sharply thereafter (such as CO and HCN), and those which peak at higher densities and then remain roughly constant (e.g. NH$_3$, N$_2$H$^+$). Evolving the chemistry with physical properties held constant at their final values results in a significant overestimation of gas-phase abundances for all molecules, and does not capture the drastic variations in abundance caused by different evolutionary histories. The dynamical evolution of molecular gas cannot be neglected when modelling its chemistry.Comment: 14 pages, 13 figures. MNRAS accepte

Observations of the BL Lac Object 3C 66A with STACEE

We present the analysis and results of recent high-energy gamma-ray observations of the BL Lac object 3C 66A conducted with the Solar Tower Atmospheric Cherenkov Effect Experiment (STACEE). During the 2003-2004 observing season, STACEE extensively observed 3C 66A as part of a multiwavelength campaign on the source. A total of 33.7 hours of data was taken on the source, plus an equivalent-duration background observation. After cleaning the data set a total of 16.3 hours of live time remained, and a net on-source excess of 1134 events was seen against a background of 231742 events. At a significance of 2.2 standard deviations this excess is insufficient to claim a detection of 3C 66A, but is used to establish flux upper limits for the source.Comment: Accepted for publication in the Astrophysical Journa

Spectrum and Variability of Mrk501 as observed by the CAT Imaging Telescope

The CAT Imaging Telescope has observed the BL Lac object Markarian 501 between March and August 1997. We report here on the variability over this time including several large flares. We present also preliminary spectra for all these data, for the low emission state, and for the largest flare.Comment: 4 pages, 4 figures, Late