84 research outputs found
Transient Receptor Potential Vanilloid 1 in animal tissues: An overview to highlight similarities and differences with human species
MEASUREMENT OF ALDOSTERONURIA IN HEALTHY AND CARDIOPATHIC DOGS: EARLY EVALUATION OF TWO ELISA METHODS
Effects of different housing systems on haematological profile, salivary cortisol concentration, and behavioural stress responses in calves of different ages
Transient Receptor Potential Vanilloid 1 Expression and Functionality in MCF-7 Cells: A Preliminary Investigation
PURPOSE: Transient receptor potential vanilloid 1 (TRPV1) is a nonselective cation channel belonging to the transient receptor potential family, and it is expressed in different neoplastic tissues. Its activation is associated with regulation of cancer growth and progression. The aim of this research was to study the expression and pharmacological characteristics of TRPV1 in cells derived from human breast cancer MCF-7 cells. METHODS: TRPV1 presence was assessed by binding studies and Western blotting. Receptor binding characteristics were evaluated through competition assays, while 3-(4,5-dimethylthiazol-2-yl)-2,5,-dipheyltetrazolium bromide reduction assays were performed to confirm an early hypothesis regarding the modulation of cancer cell proliferation. The functionality of TRPV1 was evaluated by measuring Ca(2+) uptake in the presence of increasing concentrations of TRPV1 agonists and antagonists. RESULTS: Binding studies identified a single class of TRPV1 (B(max) 1,492±192 fmol/mg protein), and Western blot showed a signal at 100 kDa corresponding to the molecular weight of human TRPV1. Among the different tested agonists and antagonists, anandamide (Ki: 2.8×10(-11) M) and 5-iodoresiniferatoxin (5-I-RTX) (Ki: 5.6×10(-11) M) showed the highest degrees of affinity for TRPV1, respectively. All tested TRPV1 agonists and antagonists caused a significant (p<0.05) decrease in cell growth rate in MCF-7 cells. For agonists and antagonists, the efficacy of tested compounds displayed the following rank order: resiniferatoxin>anandamide>capsaicin and 5-I-RTX=capsazepine, respectively. CONCLUSION: These data indicate that both TRPV1 agonists and antagonists induce significant inhibition of MCF-7 cell growth. Even though the mechanisms involved in the antiproliferative effects of TRPV1 agonists and antagonists should be further investigated, it has been suggested that agonists cause desensitization of the receptor, leading to alteration in Ca(2+)-influx regulation. By contrast, antagonists cause a functional block of the receptor with consequent fatal dysregulation of cell homeostasis
Changes in Serum Blood Parameters in Farmed Rainbow Trout (Oncorhynchus mykiss) Fed with Diets Supplemented with Waste Derived from Supercritical Fluid Extraction of Sweet Basil (Ocimum basilicum)
Concentrations of platelet α2-adrenoceptors, lymphocyte muscarinic receptors, and blood monoamines in dogs (Canis familiaris) affected by canine cognitive dysfunction syndrome
Detection of Leptospira spp. in Water Turtle (Trachemys scripta) Living in Ponds of Urban Parks
Urban parks are green areas of cities where families and children spend hours outside. Turtles often inhabit urban parks. However, even if the animals seem harmless, they may serve as both reservoirs or accidental hosts for different serotypes of Leptospira spp. Leptospira spp. is a waterborne zoonotic bacterium relevant for public health. Reptiles and amphibians may play a role in the epidemiology, transmission, and persistence of Leptospira spp. In the present study, we observed the presence of anti-leptospiral agglutinins in a group of freshwater turtles (Trachemys scripta) captured in three urban ponds of the metropolitan city of Turin, Italy
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