Prenatal exposure to tobacco smoke leads to increased mitochondrial DNA content in umbilical cord serum associated to reduced gestational age

<p>We investigated if prenatal exposures to tobacco smoke lead to changes in mitochondrial DNA content (mtDNA) in cord serum and adversely affect newborns’ health. Umbilical cord serum cotinine levels were used to determine in utero exposure to smoking. Cord serum mtDNA was measured by quantitative polymerase chain reaction analysis of the genes coding for cytochrome c oxidase1 (<i>MT</i>-<i>CO1</i>) and cytochrome c oxidase2 (<i>MT</i>-<i>CO2</i>). Log transformed levels of mtDNA coding for <i>MT</i>-<i>CO1</i> and <i>MT</i>-<i>CO2</i> were significantly higher among infants of active smokers with higher serum level of cotinine (<i>p</i> < 0.05) and inversely associated with gestational age (<i>p</i> = 0.08; <i>p</i> = 0.02). Structural equation modeling results confirmed a positive association between cotinine and <i>MT</i>-<i>CO1</i> and2 (<i>p</i> < 0.01) and inverse associations with gestational age (<i>p</i> = 0.02) and <i>IGF</i>-<i>1</i> (<i>p</i> < 0.01). We identified a dose-dependent increase in the level of <i>MT</i>-<i>CO1</i> and <i>MT</i>-<i>CO2</i> associated to increased cord serum cotinine and decreased gestational age.</p

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11 février 19401940/02/11 (A69).Appartient à l’ensemble documentaire : PoitouCh

Additional file 8: of Macrophage migration inhibitory factor - a therapeutic target in gallbladder cancer

Representative images of MIF by immunohistochemistry. Representative sections from cholecystitis tissues (moderate staining) – (i) stained with hematoxylin and eosin; (ii) probed with anti-MIF antibody. Representative sections from gallbladder adenocarcinoma tissue (weak staining); (iii) stained with hematoxylin and eosin; (iv) probed with anti-MIF antibody. (PDF 1714 kb

Additional file 1: of Macrophage migration inhibitory factor - a therapeutic target in gallbladder cancer

Properties of GBC cell lines used in the study. (XLS 27 kb