BRIGHT ASTEROIDS IN THE RED SEA - AN EMERGING PATHOGEN CANDIDA HAEMOLUNII: ARE AZOLES THE TREATMENT OPTION?

This is a case study of a 15-year-old female who on chemotherapy and central line catheter-related bloodstream candidemia presented with recurrentepisodes of candidemia, was febrile and breathless. She was diagnosed of Candida haemulonii fungemia. Echinocandins was started as this species ofCandida is usually resistant to the commonly used antifungal agents. On treatment, the patient was afebrile and discharged.Keywords: Candida haemulonii, Candidemia, Case report, Emerging pathogenÂ

Value of dual testing for identifying tuberculous infection

Setting: A rural community in Chingleput district in Tamil Nadu state in south India. Objective: To determine the value of dual testing with PPD-S and PPD-B for identifying subjects with a tuberculous infection. Design: About 240,000 subjects in rural south India, all of whom were tested initially with PPD-S and PPD-B, were followed up for 15 years, mainly by total population survey once in every 212 years. The incidence of culture-positive tuberculosis was estimated using life-table technique. Results: Among 17,530 subjects with an intermediate reaction (8–11 mm) to PPD-S at intake, 285 with an induration to PPD-S exceeding the induration to PPD-B by at least 2 mm, had a significantly higher incidence of culture-positive tuberculosis than the remaining (154 and 93 per 100,000), and similarly 481 who had an induration of o10mm to PPD-B compared to those with X10mm (131 and 93 per 100,000). These subjects may be regarded as having a tuberculous infection. Infection with non-tuberculous mycobacteria conferred protection of about 30% against the development of tuberculosis over a 15-year period. Conclusion: In subjects with an intermediate reaction (8–11 mm) to PPD-S, dual testing with PPD-B enabled identification of those with a tuberculous infection. Most of the reactions were due to non-tuberculous mycobacteria

Investigations on the liquid crystalline phases of cation-induced condensed DNA

Viral and nonviral condensing agents are used in gene therapy to compact oligonucleotides and plasmid DNA into nanostructures for their efficient transport through the cell membranes. Whereas viral vectors are best by the toxic effects on the immune system, most of the nonviral delivery vehicles are not effective for use in clinical system. Recent investigations indicate that the supramolecular organization of DNA in the condensed state is liquid crystalline. The present level of understanding of the liquid crystalline phase of DNA is inadequate and a thorough investigation is required to understand the nature, stability, texture and the influence of various environmental conditions on the structure of the phase. The present study is mainly concerned with the physicochemical investigations on the liquid crystalline transitions during compaction of DNA by cationic species such as polyamines and metallic cations. As a preliminary to the above investigation, studies were conducted on the evolution of mesophase transitions of DNA with various cationic counterion species using polarized light microscopy. These studies indicated significant variations in the phase behaviour of DNA in the presence of Li and other ions. Apart from the neutralization of the charges on the DNA molecule, these ions are found to influence selectively the hydration sphere of DNA that in turn influences the induction and stabilization of the LC phases. The higher stability observed with the liquid crystalline phases of Li-DNA system could be useful in the production of nanostructured DNA. In the case of the polyamine, a structural specificity effect depending on the nature, charge and structure of the polyamine used has been found to be favoured in the crystallization of DNA

Metastasis-Associated protein 1 is an upstream regulator of DNMT3a and stimulator of insulin-growth factor binding protein-3 in breast cancer.

Despite a recognized role of DNA methyltransferase 3a (DNMT3a) in human cancer, the nature of its upstream regulator(s) and relationship with the master chromatin remodeling factor MTA1, continues to be poorly understood. Here, we found an inverse relationship between the levels of MTA1 and DNMT3a in human cancer and that high levels of MTA1 in combination of low DNMT3a status correlates well with poor survival of breast cancer patients. We discovered that MTA1 represses DNMT3a expression via HDAC1/YY1 transcription factor complex. Because IGFBP3 is an established target of DNMT3a, we investigated the effect of MTA1 upon IGFBP3 expression, and found a coactivator role of MTA1/c-Jun/Pol II coactivator complex upon the IGFBP3 transcription. In addition, MTA1 overexpression correlates well with low levels of DNMT3a which, in turn also correlates with a high IGFBP3 status in breast cancer patients and predicts a poor clinical outcome for breast cancer patients. These findings suggest that MTA1 could regulate the expression of IGFBP3 in both DNMT3a-dependent and -independent manner. Together findings presented here recognize an inherent role of MTA1 as a modifier of DNMT3a and IGFBP3 expression, and consequently, the role of MTA1-DNMT3a-IGFBP3 axis in breast cancer progression

Prospective study of the functional outcome of uncemented bipolar hemiarhroplasty in displaced fracture neck of femur in elderly

Background: Hip fractures in older patients are associated with impaired mobility, excess morbidity, mortality and loss of independence. To avoid the drawbacks of internal fixation and for early mobilization, hemiarthroplasty is performed in elderly. The bipolar prosthesis was developed to overcome the shortcomings of the unipolar fixed-head prosthesis. An additional site of motion would be expected to decrease the amount of loosening, dislocation, and head migration. The aim of the study is to assess the functional outcome of the patients with fracture neck of femur treated with bipolar hemiarthroplasty.Methods: In this study, 20 patients above the age of 60 years with acute displaced fracture neck of femur underwent hemireplacement arthroplasty with bipolar prosthesis in Department of Orthopaedics, KIMS, Bangalore. The patients were followed up on 6th, 12th, 18th, 24th week post operatively. Functional outcome was assessed with Harris hip score.Results: In our study, mean average age was 72.3 years with predominantly female patients with 75% of the patients with left sided fracture. 95% of the patients had slight or no pain, 85% of patients had slight or no limp. 50% of the patients could walk without support, 65% of the patients could walk a distance of 6 blocks or more. Average Harris hip score was 82%.Conclusions: These results suggest functional outcome following bipolar hemiarthroplasty is good. Early mobilisation, pain free hip and with good level of activity is obtained following bipolar hemiarthroplasty

Sensitivity of WRF cloud microphysics to simulations of a severe thunderstorm event over southeast India

In the present study, we have used the Weather Research and Forecasting (WRF) model to simulate the features associated with a severe thunderstorm observed over Gadanki (13.5° N, 79.2° E), over southeast India, on 21 May 2008 and examined its sensitivity to four different microphysical (MP) schemes (Thompson, Lin, WSM6 and Morrison). We have used the WRF model with three nested domains with the innermost domain of 2 km grid spacing with explicit convection. The model was integrated for 36 h with the GFS initial conditions of 00:00 UTC, 21 May 2008. For validating simulated features of the thunderstorm, we have considered the vertical wind measurements made by the Indian MST radar installed at Gadanki, reflectivity profiles by the Doppler Weather Radar at Chennai, and automatic weather station data at Gadanki.There are major differences in the simulations of the thunderstorm among the MP schemes, in spite of using the same initial and boundary conditions and model configuration. First of all, all the four schemes simulated severe convection over Gadanki almost an hour before the observed storm. The DWR data suggested passage of two convective cores over Gadanki on 21 May, which was simulated by the model in all the four MP schemes. Comparatively, the Thompson scheme simulated the observed features of the updraft/downdraft cores reasonably well. However, all the four schemes underestimated strength and vertical extend of the updraft cores. The MP schemes also showed problems in simulating the downdrafts associated with the storm. While the Thompson scheme simulated surface rainfall distribution closer to observations, the other three schemes overestimated observed rainfall. However, all the four MP schemes simulated the surface wind variations associated with the thunderstorm reasonably well. The model simulated reflectivity profiles were consistent with the observed reflectivity profile, showing two convective cores. These features are consistent with the simulated condensate profiles, which peaked around 5-6 km. As the results are dependent on initial conditions, in simulations with different initial conditions, different schemes may become closer to observations. The present study suggests not only large sensitivity but also variability of the microphysical schemes in the simulations of the thunderstorm. The study also emphasizes the need for a comprehensive observational campaign using multi-observational platforms to improve the parameterization of the cloud microphysics and land surface processes over the Indian region

Active community surveillance of the impact of different tuberculosis control measures, Tiruvallur, South India, 1968-2001

Background: Tuberculosis is curable, but community surveys documenting epidemiological impact of the WHO-recommended DOTS strategy on tuberculosis prevalence have not been published. We used active community surveillance to compare the impact of DOTS with earlier programmes. Methods: We conducted tuberculosis disease surveys using random cluster sampling of a rural population in South India approximately every 2.5 years from 1968 to 1986, using radiography as a screening tool for sputum examination. In 1999, DOTS was implemented in the area. Prevalence surveys using radiography and symptom screening were conducted at the start of DOTS implementation and after 2.5 years. Results: From 1968 to 1999, culture-positive and smear-positive tuberculosis declined by 2.3 and 2.5% per annum compared with 11.9 and 5.6% after DOTS implementation. The 2.5 year period of DOTS implementation accounted for one-fourth of the decline in prevalence of culture-positive tuberculosis over 33 years. Multivariate analysis showed that prevalence of culture-positive tuberculosis decreased substantially (10.0% per annum, 95% CI: 2.8–16.6%) owing to DOTS after only slight declines related to temporal trends (2.1% annual decline, 95% CI: 1.1–3.2%) and short-course chemotherapy (1.5% annual decline, 95% CI: �9.7% to 11.5%). Under DOTS, the proportion of total cases identified through clinical care increased from 81 to 92%. Conclusions: Following DOTS implementation, prevalence of culture-positive tuberculosis decreased rapidly following a gradual decline for the previous 30 years. In the absence of a large HIV epidemic and with relatively low levels of rifampicin resistance, DOTS was associated with rapid reduction of tuberculosis prevalenc

The Virulence in the Guinea-pig of Tubercle Bacilli Isolated before Treatment from South Indian Patients with Pulmonary Tuberculosis: 1. Homogeneity of the Investigation and a Critique of the Virulence Test

A series of studies on the virulence in the guinea-pig of tubercle bacilli isolated before treatment from Indian tuberculous patients admitted to a controlled comparison of different regimens of domiciliary chemotherapy has recently been undertaken by the Tuberculosis Chemotherapy Centre, Madras. The main object of these studies was to determine whether the differences in virulence of the tubercle bacilli obtained from Indian patients before the start of chemotherapy were related to the severtiy or type of the patients’ disease at that time and to the subsequent response to treatment. Before these relationships could be‘ investigated, however, it was necessary to find out whether the results of the virulence tests, which were carried out over a period of two-and-a-half years at the Centre and at the Microbiological Research Establishment, Porton, England, could be considered as a unified whole-that, is, as if they had all been done on the same day in the same laboratory. A proportion of the cultures was stored at – 20°C for 44-78 weeks, but this did not affect their virulence. Inter-experimental variation was found to be small in the Porton series of tests and undetectable in the Madras series, and the results in the latter series could be successfully adjusted to those in the former by allowing for differences in the means and standard deviations of the distributions for the two series. The measure of virulence used was found to be reasonably acceptable for the analysis of variance technique. Suggestions are made as to ways of improving the efficiency of the experimental design in future studies

An assessment of the bottom-trawl fishery resources of the northeast coast of India

An overview of the bottom trawl fishery resources of the continental shelf of the northeast coast (lat. 15° N-21° N and long. 80^ E-83° El has been attempted based on data collected from the exploratory fishery surveys conducted by the Govt. of India fishing vessels during 1961-1985. The 'swapt-area' method has been employed to estimate the standing stock, and 60% of this has bean reckoned as the potential yield The catch rates in the shelf region ranged between 1 l<g/hr in square 17-33 CI and 377 kg/hr in squares that yielded 150kg/hr or more are distributed widely both in the inshore and offshore grounds- The potential yield estimates for the area explored varied between 083 t/km2 in 20° H-Sl" E and 3 37 t/km^ in 19° N-35°