1 research outputs found
Anti-Staphylococcal Biofilm Effects of Human Cathelicidin Peptides
<i>Staphylococcus aureus</i> can live together in the
form of biofilms to avoid elimination by the host. Thus, a useful
strategy to counteract bacterial biofilms is to re-engineer human
antimicrobial peptide LL-37 so that it can be used as a remedy for
preventing and removing biofilms. This study reports antibiofilm effects
of four human cathelicidin LL-37 peptides against community-associated
and hospital isolated methicillin-resistant <i>Staphylococcus
aureus</i> (MRSA) strains. Although the intact molecule LL-37
inhibited biofilm formation at low concentrations, it did not inhibit
bacterial attachment nor disrupt preformed biofilms. However, two
17-residue peptides, GF-17 and 17BIPHE2, inhibited bacterial attachment,
biofilm growth, and disrupted established biofilms. An inactive peptide
RI-10 was used as a negative control. Our results obtained using the <i>S. aureus</i> mutants in a static biofilm model are consistent
with the literature obtained in a flow cell biofilm model. Because
17BIPHE2 is the most effective biofilm disruptor with desired stability
to proteases, it is a promising lead for developing new anti-MRSA
biofilm agents