Description of two biovars in the Rhizobium galegae species: biovar orientalis and biovar officinalis

Summary Twenty-six Rhizobium galegae strains, representing the center of origin of the host plants Galega orientalis and G. officinalis as well as other geographic regions, were used in a polyphasic analysis of the relationships of R. galegae strains. Phage typing, lipopolysaccharide (LPS) profiling, pulsed field gel electrophoresis (PFGE) profiling and rep-PCR (use of repetitive sequences as PCR primers for genomic fingerprinting) with REP and ERIC primers investigated nonsymbiotic properties, whereas plasmid profiling and hybridisation with a nif gene probe, and with nodB, nodD, nod box and an IS sequence from the symbiotic region as probes, were used to reveal the relationships of symbiotic genes. The results were used in pairwise calculations of distances between the strains, and the distances were visualised as a dendrogram. Indexes of association were compared for all tests pooled, and for chromosomal tests and symbiotic markers separately, to display the input of the different categories of tests on the grouping of the strains. Our study shows that symbiosis related genetic traits in R. galegae divide strains belonging to the species into two groups, which correspond to strains forming an effective symbioses with G. orientalis and G. officinalis respectively. We therefore propose that Rhizobium galegae strains forming an effective symbiosis with Galega orientalis are called R. galegae bv. orientalis and strains forming an effective symbiosis with Galega officinalis are called R. galegae bv. officinalis

Dynamic change of NT- PROBNP in patients with ST-elevation myocardial infarction treated with primary coronary intervention

In the recent years important biomarkers have  emerged as  tools for diagnosis and risk stratification in cardiovascular diseases. Such markers are B- type natriuretic peptide ( BNP) and N- terminal B- type  natriuretic peptide (NT-proBNP).Peripheral blood for the serum levels of  NT-proBNP was taken from patients with STEMI before PCI and 24-48hours after the onset of the symptoms of myocardial infarction. Three of the samples  from all 53 turned out to be positive to NT - proBNP  concentration on Day 0. On day 1 there was   a significant  elevation of the positive samples 11 from 53 patients ( 20,7%) ( p = 0,01). All  patients with STEMI and elevated serum levels of NT- proBNP  have left ventricular ejection fraction <50%.  Our results imply that  NT -proBNP level and its increase in the serum  may be used as a biomarker for the severity of the ischemic heart disease

Overexpression of the integrin-linked kinase (ILK) promotes anchorage-independent cell cycle progression

grantor: University of TorontoOverexpression of ILK in rat epithelial cells results in decreased cell adhesion and anchorage-independent growth. I hypothesized, therefore, that overexpression of ILK would directly or indirectly affect the cell cycle machinery. Cell adhesion to substratum has been shown to regulate cyclin A expression as well as cyclin D and E-dependent kinases. Here, I demonstrate that stable transfection and overexpression of the Integrin Linked Kinase (ILK) induces anchorage-independent cell cycle progression but not serum-independent growth of IEC18 rat intestinal epithelial cells. ILK overexpression results in increased expression of cyclin D1 and cyclin A, activation of cdk4 and cyclin E-associated kinases, and hyperphosphorylation of the retinoblastoma protein. In addition, p21 and p27 cdk inhibitors display altered electrophoretic mobilities, with p27 having reduced inhibitory activity. These results indicate that, when overexpressed, ILK induces signalling pathways resulting in the stimulation of G1/S cyclin-cdk activities which are normally regulated by cell adhesion and integrin engagement.M.Sc

Blockchain Software Selection as a Fuzzy Multi-Criteria Problem

Increased consumer requirements for quality, safety and traceability of goods in supply chains has accelerated the implementation of blockchain during the COVID-19 pandemic. The right choice of blockchain software is a complicated task and an important prerequisite for successful deployment. In this study, we propose a conceptual framework for group multi-criteria selection of blockchain software in fuzzy environment according to organization needs and experts’ judgements. The applicability of the new framework has been verified through an illustrative example for ranking blockchain systems. The evaluations of compared alternatives were calculated by using measurement of alternatives and ranking according to the compromise solution (MARCOS) method. The robustness of the new framework was proven by sensitivity analysis in which two (crisp and fuzzy) MARCOS models with two different sets of weighting coefficients were compared

Distribution of microbial abundance in long-term copper contaminated soils from Topolnitsa-Pirdop valley, Southern Bulgaria

This study presents the distribution of bacterial and fungal abundances in long-term copper (Cu) contaminated soils in Topolnitsa-Pirdop valley – a highly industrialized zone with a number of mines and processing plants for copper and other non-ferrous metals. The bacterial (16S rRNA gene copies) and fungal (ITS rRNA gene copies) were estimated using quantitative PCR technique in five topsoils, differently Cu contaminated (ranging from 28.05 to 198.9 mg kg-1). Bacterial abundance varied in a range of 1.68 × 1011 to 3.24 × 101116S rRNA genes, whereas fungi amounted from 1.95 × 108 to 6.71 × 108 ITS rRNA genes. Fungal and bacterial abundances were significantly (fungi) and insignificantly (bacteria) influenced by Cu contamination. The fungal/bacterial ratio related negatively with soil Cu, which shifted microbial communities’ structure towards bacterial dominance. Since the ratio between bacteria and fungi are vital in explaining many soil functions, the calculated changes in this ratio indicated deterioration in soil quality, being of primary importance for plant production

Differences in bacterial functional profiles from loamy sand and clay loam textured soils under fungicide QuadrisR impact

The non-target effect of the fungicide QuadrisR on the bacterial community from grassland loamy sand (LS) and cropland clay loam (CL) soils with unknown history of fungicide usage was investigated. QuadrisR was applied to soil mesocosms at 0.0 mg kg-1 (Az0), 2.90 mg kg-1 (Az1), 14.65 mg kg-1 (Az2) and 35.0 mg kg-1 (Az3) calculated towards the active ingredient azoxystrobin (Az). Response of bacterial communities to QuadrisR was investigated during a 120-day incubation experiment, evaluating the shifts in bacterial catabolic profiles by the community-level physiological profiling (CLPP) technique and Biolog EcoPlatesTM method. QuadrisR decreased the overall catabolic activity (AWCD) of soil bacterial communities and the rate of decrease was independent of soil type and fungicide concentration. Fungicide affected negatively the utilisation of amines and positively that of amino acids in both soil types, whereas the effects on other carbon guilds (carbohydrates, carboxylic acids and polymers) corresponded closely to the respective soil type and fungicide concentration. Results indicated the presence of non-target effects of QuadrisR on bacterial functioning; hence, it is important to address the fungicide side-effects on soil health

Correlation between bacterial abundance, soil properties and heavy metal contamination in the area of non-ferrous metal processing plant, Southern Bulgaria

In the present study, the correlation between bacterial abundance and soil physicochemical properties along the heavy metal contamination gradient in the area of non-ferrous metal processing plant was assessed. Our results showed that bacterial abundance (number of heterotrophic bacteria and number of 16S rRNA gene copies) decreased with 45–56% (CFU) and 54–87% (16S rRNA gene) along the Zn, Pb and Cd contamination gradient. The total bacterial abundance (16S rRNA gene) increased exponentially in contrast to the abundance of heterotrophic bacteria. The reduction of bacterial abundance in heavily contaminated soil indicated that the soil properties (soil pH, total organic carbon, inorganic ions, soil texture) could modify the effects of heavy metals and the response of microorganisms to that stress in long-term contaminated soils

Cell adhesion and the integrin-linked kinase regulate the LEF-1 and β-catenin signaling pathways

The integrin-linked kinase (ILK) is an ankyrin repeat containing serine-threonine protein kinase that can interact directly with the cytoplasmic domains of the β1 and β3 integrin subunits and whose kinase activity is modulated by cell–extracellular matrix interactions. Overexpression of constitutively active ILK results in loss of cell–cell adhesion, anchorage-independent growth, and tumorigenicity in nude mice. We now show that modest overexpression of ILK in intestinal epithelial cells as well as in mammary epithelial cells results in an invasive phenotype concomitant with a down-regulation of E-cadherin expression, translocation of β-catenin to the nucleus, formation of a complex between β-catenin and the high mobility group transcription factor, LEF-1, and transcriptional activation by this LEF-1/β-catenin complex. We also find that LEF-1 protein expression is rapidly modulated by cell detachment from the extracellular matrix, and that LEF-1 protein levels are constitutively up-regulated at ILK overexpression. These effects are specific for ILK, because transformation by activated H-ras or v-src oncogenes do not result in the activation of LEF-1/β-catenin. The results demonstrate that the oncogenic properties of ILK involve activation of the LEF-1/β-catenin signaling pathway, and also suggest ILK-mediated cross-talk between cell–matrix interactions and cell–cell adhesion as well as components of the Wnt signaling pathway