Brightfield images of PCO 162 demonstrating the effects of selective seeding.

<p>A: Epithelial cell growth from supernatant removed 30 minutes after initial plating (×20 magnification); B: Cells adhering during the initial 30 minute incubation were almost entirely fibroblastic (×10 magnification).</p

Comparison between the characterisation results from immunoflourescent microscopy (IF) and Imagestream^X (IS^X).

<p>Comparison between the characterisation results from immunoflourescent microscopy (IF) and Imagestream^X (IS^X).</p

Growth curves for 6 representative PCO cultures (PCO143, 146, 149, 164,167 and 168).

<p>The mean and SD of 6 repeats for each time point is plotted, normalised to day 1.</p

Phenotypic appearances of primary cultures.

<p>A: Brightfield demonstrating cobblestone monolayer; immunoflourescent images with antibodies targeted against: B: FITC-anti-pancytokeratin; C: Alexafluor 596 anti-CA125; D: Alexflour 488 anti-EpCAM; E: Alexafluor 596 anti-MOC 31; F: Alexaflour 596 anti-Vimentin</p

Patient demographics by histological subtype.

<p>Patient demographics by histological subtype.</p

Antigen expression in primary cultures.

<p>PCO 158 A: Immunohistochemical detection of CA125 in FFPE tissue; B: Immunofluourescent detection of CA125 with Alexaflour596 from corresponding primary culture</p

HR DNA repair status of PCO culture with corresponding sensitivity to 10 µM Rucaparib.

<p>*Cell survival following 10 µM Rucaprib. Resistant (R)  =  more than 70% survival; Sensitive (S)  =  less than 70% survival.</p

Figure 6

<p>A: ImagestreamX cell plot of representative cells demonstrating the three major cellular sub-populations within ascitic fluid, ; i) CK positive only, ii) CK and CA125 positive and iii) EpCAM, CK and CA125 positive. B: Sub-populations identified in the epithelial cell population in EOC ascitic fluid (n = the total number of epithelial cells identified in 10 ml of ascites).</p

Primary culture outcomes following transport.

<p>Primary culture outcomes following transport.</p

Antibodies used for cell characterisation.

<p>Antibodies used for cell characterisation.</p