4 research outputs found
A Carbon Nanotube Optical Sensor Reports Nuclear Entry <i>via</i> a Noncanonical Pathway
Single-walled
carbon nanotubes are of interest in biomedicine for
imaging and molecular sensing applications and as shuttles for various
cargos such as chemotherapeutic drugs, peptides, proteins, and oligonucleotides.
Carbon nanotube surface chemistry can be modulated for subcellular
targeting while preserving photoluminescence for label-free visualization
in complex biological environments, making them attractive materials
for such studies. The cell nucleus is a potential target for many
pathologies including cancer and infectious diseases. Understanding
mechanisms of nanomaterial delivery to the nucleus may facilitate
diagnostics, drug development, and gene-editing tools. Currently,
there are no systematic studies to understand how these nanomaterials
gain access to the nucleus. Herein, we developed a carbon nanotube
based hybrid material that elucidate a distinct mechanism of nuclear
translocation of a nanomaterial in cultured cells. We developed a
nuclear-targeted probe <i>via</i> cloaking photoluminescent
single-walled carbon nanotubes in a guanidinium-functionalized helical
polycarbodiimide. We found that the nuclear entry of the nanotubes
was mediated by the import receptor importin β without the aid
of importin α and not by the more common importin α/β
pathway. Additionally, the nanotube photoluminescence exhibited distinct
red-shifting upon entry to the nucleus, potentially functioning as
a reporter of the importin β-mediated nuclear transport process.
This work delineates a noncanonical mechanism for nanomaterial delivery
to the nucleus and provides a reporter for the study of nucleus-related
pathologies
A Carbon Nanotube Optical Sensor Reports Nuclear Entry <i>via</i> a Noncanonical Pathway
Single-walled
carbon nanotubes are of interest in biomedicine for
imaging and molecular sensing applications and as shuttles for various
cargos such as chemotherapeutic drugs, peptides, proteins, and oligonucleotides.
Carbon nanotube surface chemistry can be modulated for subcellular
targeting while preserving photoluminescence for label-free visualization
in complex biological environments, making them attractive materials
for such studies. The cell nucleus is a potential target for many
pathologies including cancer and infectious diseases. Understanding
mechanisms of nanomaterial delivery to the nucleus may facilitate
diagnostics, drug development, and gene-editing tools. Currently,
there are no systematic studies to understand how these nanomaterials
gain access to the nucleus. Herein, we developed a carbon nanotube
based hybrid material that elucidate a distinct mechanism of nuclear
translocation of a nanomaterial in cultured cells. We developed a
nuclear-targeted probe <i>via</i> cloaking photoluminescent
single-walled carbon nanotubes in a guanidinium-functionalized helical
polycarbodiimide. We found that the nuclear entry of the nanotubes
was mediated by the import receptor importin β without the aid
of importin α and not by the more common importin α/β
pathway. Additionally, the nanotube photoluminescence exhibited distinct
red-shifting upon entry to the nucleus, potentially functioning as
a reporter of the importin β-mediated nuclear transport process.
This work delineates a noncanonical mechanism for nanomaterial delivery
to the nucleus and provides a reporter for the study of nucleus-related
pathologies
A Carbon Nanotube Optical Sensor Reports Nuclear Entry <i>via</i> a Noncanonical Pathway
Single-walled
carbon nanotubes are of interest in biomedicine for
imaging and molecular sensing applications and as shuttles for various
cargos such as chemotherapeutic drugs, peptides, proteins, and oligonucleotides.
Carbon nanotube surface chemistry can be modulated for subcellular
targeting while preserving photoluminescence for label-free visualization
in complex biological environments, making them attractive materials
for such studies. The cell nucleus is a potential target for many
pathologies including cancer and infectious diseases. Understanding
mechanisms of nanomaterial delivery to the nucleus may facilitate
diagnostics, drug development, and gene-editing tools. Currently,
there are no systematic studies to understand how these nanomaterials
gain access to the nucleus. Herein, we developed a carbon nanotube
based hybrid material that elucidate a distinct mechanism of nuclear
translocation of a nanomaterial in cultured cells. We developed a
nuclear-targeted probe <i>via</i> cloaking photoluminescent
single-walled carbon nanotubes in a guanidinium-functionalized helical
polycarbodiimide. We found that the nuclear entry of the nanotubes
was mediated by the import receptor importin β without the aid
of importin α and not by the more common importin α/β
pathway. Additionally, the nanotube photoluminescence exhibited distinct
red-shifting upon entry to the nucleus, potentially functioning as
a reporter of the importin β-mediated nuclear transport process.
This work delineates a noncanonical mechanism for nanomaterial delivery
to the nucleus and provides a reporter for the study of nucleus-related
pathologies
A Carbon Nanotube Optical Sensor Reports Nuclear Entry <i>via</i> a Noncanonical Pathway
Single-walled
carbon nanotubes are of interest in biomedicine for
imaging and molecular sensing applications and as shuttles for various
cargos such as chemotherapeutic drugs, peptides, proteins, and oligonucleotides.
Carbon nanotube surface chemistry can be modulated for subcellular
targeting while preserving photoluminescence for label-free visualization
in complex biological environments, making them attractive materials
for such studies. The cell nucleus is a potential target for many
pathologies including cancer and infectious diseases. Understanding
mechanisms of nanomaterial delivery to the nucleus may facilitate
diagnostics, drug development, and gene-editing tools. Currently,
there are no systematic studies to understand how these nanomaterials
gain access to the nucleus. Herein, we developed a carbon nanotube
based hybrid material that elucidate a distinct mechanism of nuclear
translocation of a nanomaterial in cultured cells. We developed a
nuclear-targeted probe <i>via</i> cloaking photoluminescent
single-walled carbon nanotubes in a guanidinium-functionalized helical
polycarbodiimide. We found that the nuclear entry of the nanotubes
was mediated by the import receptor importin β without the aid
of importin α and not by the more common importin α/β
pathway. Additionally, the nanotube photoluminescence exhibited distinct
red-shifting upon entry to the nucleus, potentially functioning as
a reporter of the importin β-mediated nuclear transport process.
This work delineates a noncanonical mechanism for nanomaterial delivery
to the nucleus and provides a reporter for the study of nucleus-related
pathologies