Pomegranate peel extract inhibits internalization and replication of the influenza virus: An in vitro study

Objective: Influenza virus, which is associated with high level of morbidity and mortality, has been recently considered a public health concern; however, the methods of choice to control and treat it are limited. Our previous study showed anti-influenza virus activity of pomegranate peel extract (PPE). In this study, the mechanism through which PPE acts against influenza virus A/Puerto Rico/8/34 (H1N1; PR8) was investigated. Materials and Methods: Ethyl alcohol extract of pomegranate (Punica granatum L.) peel was prepared, and the action mechanism of PPE in inhibiting influenza replication was studied by time-ofdrug-addition assay, virucidal activity, RNA replication, hemagglutination inhibition assay, viral mRNA expression, and western blot analysis. Results: PPE inhibited viral polymerase activity, viral RNA replication, and viral protein expression but could not affect hemagglutination inhibition and virucidal activity. According to time-of-drug-addition assay results, PPE inhibited the virus adsorption and early steps of influenza replication. Conclusion: This study demonstrated that the antiviral effect of PPE on influenza virus is most probably associated with inhibition of viral adsorption and viral RNA transcription. Keywords: Anti-influenza virus; Pomegranate; Punica granatum L.; Mechanism

One-year prevalence of antimicrobial susceptibility pattern of methicillin-resistant Staphylococcus aureus recovered from raw meat

Food with animal origins and especially meat may play an important role in transmission of methicillin-resistant strains of Staphylococcus aureus to humans. The present investigation was carried out to determine the prevalence of MRSA in various types of Iranian meat samples as well as study their antimicrobial resistance properties. Nine-hundred raw meat samples were collected during various months of the year. Samples were cultured and those that were MRSA-positive were subjected to the disk diffusion method to study the antibiotic resistance pattern. One-hundred and sixty out of 900 raw meat samples (17.7%) were positive for MRSA. Raw sheep meat samples had the highest (24.0%), while raw camel meat samples had the lowest (10%) prevalence of MRSA. Samples which were collected in June, July, August, September and June months had the highest prevalence of MRSA. Bacterial strains were also resistant to ampicillin (100%), penicillin G (100%), gatifloxacin (96.8%), ceftriaxone (80%) and oxacillin (76.2%) antibiotics. We found that only one isolate was resistant to all tested antimicrobial agents. Contaminated meat samples are potential risk factor for transmission of MRSA. Thoughtful antibiotics prescription, control the hygienic quality of meat inspections and increase the hygienic status of butchers and slaughterhouses can decrease the prevalence of MRSA in meat