Differential diagnosis of tuberculous meningitis from partially-treated pyogenic meningitis by cell ELISA

BACKGROUND: Tuberculous meningitis (TBM) is a major global health problem, and it is sometimes difficult to perform a differential diagnosis of this disease from other diseases, particularly partially-treated pyogenic meningitis (PTPM). In an earlier study, we demonstrated the presence of a 30-kD protein antigen in cerebrospinal fluid (CSF) of TBM patients. We have also shown that lymphocytes from CSF of TBM patients respond differently to this antigen than do those from PTPM patients. The purpose of this study was to develop an assay that can discriminate between TBM and PTPM. METHODS: We developed a cell enzyme-linked immunosorbant assay (Cell ELISA) to quantitatively measure production of antibodies against the 30-kD protein in B cells from CSF of TBM and PTPM patients. RESULTS: The cell ELISA yielded 92% (11/12) sensitivity and 92% (11/12) specificity for the differential diagnosis of TBM from PTPM. CONCLUSION: When induced with the 30-kD protein antigen, B cells derived from CSF of TBM patients respond to IgG production within 24 h while those derived from PTPM patients do not respond

A Simple & Convenient Solid Phase Synthesis of Bacterial Origin Octapeptide Sequence, Glu-Asp-Gly-Asn-Lys-Pro-Gly-Lys-OH

The repeating octapeptide sequence, Glu-Asp-Gly-Asn-Lys-Pro-Gly-Lys-OH derived from the glycoprotein found in Staphylococcus aureus cell wall is assembled by simple solid phase peptide synthesis methodology using a base labile linker

Genome-wide survey and analysis of microsatellites in nematodes, with a focus on the plant-parasitic species Meloidogyne incognita

<p>Abstract</p> <p>Background</p> <p>Microsatellites are the most popular source of molecular markers for studying population genetic variation in eukaryotes. However, few data are currently available about their genomic distribution and abundance across the phylum Nematoda. The recent completion of the genomes of several nematode species, including <it>Meloidogyne incognita</it>, a major agricultural pest worldwide, now opens the way for a comparative survey and analysis of microsatellites in these organisms.</p> <p>Results</p> <p>Using MsatFinder, the total numbers of 1-6 bp perfect microsatellites detected in the complete genomes of five nematode species (<it>Brugia malayi</it>, <it>Caenorhabditis elegans</it>, <it>M. hapla</it>, <it>M. incognita</it>, <it>Pristionchus pacificus</it>) ranged from 2,842 to 61,547, and covered from 0.09 to 1.20% of the nematode genomes. Under our search criteria, the most common repeat motifs for each length class varied according to the different nematode species considered, with no obvious relation to the AT-richness of their genomes. Overall, (AT)_<it>n</it>, (AG)_{<it>n </it>}and (CT)_{<it>n </it>}were the three most frequent dinucleotide microsatellite motifs found in the five genomes considered. Except for two motifs in <it>P. pacificus</it>, all the most frequent trinucleotide motifs were AT-rich, with (AAT)_{<it>n </it>}and (ATT)_{<it>n </it>}being the only common to the five nematode species. A particular attention was paid to the microsatellite content of the plant-parasitic species <it>M. incognita</it>. In this species, a repertoire of 4,880 microsatellite loci was identified, from which 2,183 appeared suitable to design markers for population genetic studies. Interestingly, 1,094 microsatellites were identified in 801 predicted protein-coding regions, 99% of them being trinucleotides. When compared against the InterPro domain database, 497 of these CDS were successfully annotated, and further assigned to Gene Ontology terms.</p> <p>Conclusions</p> <p>Contrasted patterns of microsatellite abundance and diversity were characterized in five nematode genomes, even in the case of two closely related <it>Meloidogyne </it>species. 2,245 di- to hexanucleotide loci were identified in the genome of <it>M. incognita</it>, providing adequate material for the future development of a wide range of microsatellite markers in this major plant parasite.</p

Non-Image-Forming Light Driven Functions Are Preserved in a Mouse Model of Autosomal Dominant Optic Atrophy

Autosomal dominant optic atrophy (ADOA) is a slowly progressive optic neuropathy that has been associated with mutations of the OPA1 gene. In patients, the disease primarily affects the retinal ganglion cells (RGCs) and causes optic nerve atrophy and visual loss. A subset of RGCs are intrinsically photosensitive, express the photopigment melanopsin and drive non-image-forming (NIF) visual functions including light driven circadian and sleep behaviours and the pupil light reflex. Given the RGC pathology in ADOA, disruption of NIF functions might be predicted. Interestingly in ADOA patients the pupil light reflex was preserved, although NIF behavioural outputs were not examined. The B6; C3-Opa1Q285STOP mouse model of ADOA displays optic nerve abnormalities, RGC dendropathy and functional visual disruption. We performed a comprehensive assessment of light driven NIF functions in this mouse model using wheel running activity monitoring, videotracking and pupillometry. Opa1 mutant mice entrained their activity rhythm to the external light/dark cycle, suppressed their activity in response to acute light exposure at night, generated circadian phase shift responses to 480 nm and 525 nm pulses, demonstrated immobility-defined sleep induction following exposure to a brief light pulse at night and exhibited an intensity dependent pupil light reflex. There were no significant differences in any parameter tested relative to wildtype littermate controls. Furthermore, there was no significant difference in the number of melanopsin-expressing RGCs, cell morphology or melanopsin transcript levels between genotypes. Taken together, these findings suggest the preservation of NIF functions in Opa1 mutants. The results provide support to growing evidence that the melanopsin-expressing RGCs are protected in mitochondrial optic neuropathies

An efficient MAC-based scheme against pollution attacks in XOR network coding-enabled WBANs for remote patient monitoring systems

Wireless Body Area Networks (WBANs) play a pivotal role to remote patient monitoring which is one of the main applications of m-Health. However, WBANs comprise a subset of Wireless Sensor Networks (WSNs), and thus, they inherit the limitations of WSNs in terms of communication bandwidth, reliability and power consumption that should be addressed so that WBANs can reach their full potential. Towards this direction, XOR Network Coding (NC) is a promising solution for WBANs. Nevertheless, XOR NC is vulnerable to pollution attacks, where adversaries (i.e., compromised intermediate nodes) inject into the network corrupted packets that prevent the destination nodes from decoding correctly. This has as a result not only network resource waste but also energy waste at the intermediate nodes. In this sense, pollution attacks comprise a serious threat against WBANs (i.e., resource-constrained wireless networks), that should be addressed so that WBANs can reap the benefits of XOR NC. Therefore, in this paper, we propose an efficient Message Authentication Code (MAC)-based scheme providing resistance against pollution attacks in XOR NC-enabled WBANs for remote patient monitoring systems. Our proposed scheme makes use of a number of MACs which are appended to the end of each native packet. Our results show that the proposed MAC-based scheme is more efficient compared to other competitive schemes for securing XOR NC against pollution attacks in resource-constrained wireless networks, in terms of communication bandwidth and computational complexity

Antihistamine and Wound Healing Potential of Gold Nanoparticles Synthesized Using Bulbine frutescens (L.) Willd

Marizé Cuyler,1 Danielle Twilley,1 Velaphi C Thipe,2 Vusani Mandiwana,3 Michel L Kalombo,3 Suprakas S Ray,4 Rirhandzu Shamaine Rikhotso-Mbungela,4 Arno Janse van Vuuren,5 Will Coetsee,6 Kattesh V Katti,2,&ast; Namrita Lall1,7– 9,&ast; 1Department of Plant and Soil Sciences, University of Pretoria, Pretoria, Gauteng, 0002, South Africa; 2Department of Radiology, Institute of Green Nanotechnology, University of Missouri, Columbia, MO, 65212, USA; 3Chemical Cluster Centre for Nanostructures and Advanced Materials, Council for Scientific and Industrial Research, Pretoria, 0001, South Africa; 4DST/CSIR National Centre for Nanostructured Materials, Council for Scientific and Industrial Research, Pretoria, 0001, South Africa; 5Centre for High Transmission Electron Microscopy, Nelson Mandela University, Port Elizabeth, 6031, South Africa; 6Botanica Natural Products Pty (Ltd), Canterbury Farm MR 254, Alldays, Limpopo, 0909, South Africa; 7School of Natural Resources, University of Missouri, Columbia, MO, 65211, USA; 8College of Pharmacy, JSS Academy of Higher Education and Research, Mysuru, Karnataka, 570015, India; 9Bio-Tech Research and Development Institute, University of the West Indies 770, Kingston, Jamaica&ast;These authors contributed equally to this workCorrespondence: Kattesh V Katti, Department of Radiology, Institute of Green Nanotechnology, University of Missouri, Columbia, MO 65212, USA, Tel +1 (573) 882-5656, Fax +1 (573) 884-5679, Email [email protected] Namrita Lall, Department of Plant and Soil Sciences, University of Pretoria, Pretoria, Gauteng, South Africa, Tel/Fax +27 (012) 420 2524, Email [email protected]: Atopic dermatitis (eczema) is an inflammatory skin condition with synthetic treatments that induce adverse effects and are ineffective. One of the proposed causes for the development of the condition is the outside-in hypothesis, which states that eczema is caused by a disruption in the skin barrier. These disruptions include developing dry cracked skin, which promotes the production of histamine. Bulbine frutescens (BF) is traditionally used to treat wounds and eczema; however, limited research has been conducted to scientifically validate this. Furthermore, gold nanoparticles (AuNPs) have been used to repair damaged skin; however, no research has been conducted on AuNPs synthesized using BF.Purpose: The study aimed to determine whether BF alleviated skin damage through wound healing, reducing the production of histamine and investigate whether AuNPs synthesized using BF would enhance biological activity.Methods: Four extracts and four synthesized AuNPs were prepared using BF and their antiproliferative and wound healing properties against human keratinocyte cells (HaCaT) were evaluated. Thereafter, the selected samples antiproliferative activity and antihistamine activity against phorbol 12-myristate 13-acetate (PMA) stimulated granulocytes were evaluated.Results: Of the eight samples, the freeze-dried leaf juice (BFE; p 200 μg/mL against PMA stimulated granulocytes. Compared to the untreated (media with PMA) control (0.30 ± 0.02 ng/mL), BFEAuNPs significantly inhibited histamine production at a concentration of 100 (p < 0.01) and 50 μg/mL (p < 0.001).Conclusion: BFE and BFEAuNPs stimulated wound closure, while BFEAuNPs significantly inhibited histamine production. Further investigation into BFEAuNPs in vivo wound healing activity and whether it can target histamine-associated receptors on mast cells as a potential mechanism of action should be considered. Keywords: atopic dermatitis, histamine production, outside-in hypothesis, scratch assa