The PmNAC1 gene is induced by auxin and expressed in differentiating vascular cells in callus cultures of Passiflora

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)The plant-specific NAM/ATAF/CUC (NAC) transcription factors play critical roles in diverse developmental processes such as the establishment of the shoot apical meristem, lateral root formation, flowering time, senescence, abiotic stress and defense responses. NAC domain transcription factors may be regulated at the transcriptional level by environmental clues, such as hormone concentrations. Here we report the cloning of a Passiflora morifolia putative homolog to a gene encoding a NAC-domain transcription factor, PmNAC1. We have examined the expression patterns of this gene during in vitro callus induction and growth. We observed that the induction of transcript levels of PmNAC1 is correlated to increasing concentrations of 1-naphthalene acetic acid in the culture medium. Additionally, the results of in situ hybridization experiments indicated a dynamic spatial expression pattern for PmNAC1 during callus growth, coincidental with provascular tissue differentiation, especially with xylem cell specification.152275283Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

A Passiflora homolog of a D-type cyclin gene is differentially expressed in response to sucrose, auxin, and cytokinin

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)In higher plants, one of the major components of developmental processes is cell division. The cell division cycle in plants is controlled by cyclins and cyclin-dependend kinases. Nutrient and hormonal signals can influence the roles that D-type cyclins play in the G1-to-S phase transition. Auxins and cytokinins are long known to be important plant hormones controlling plant growth. Additionally, as sucrose is the major transported carbon source in higher plants, it is possible that it plays a major role in cell division. To access the molecular aspects of the effect of auxin, cytokinin and sucrose on the regulation of cell cycle machinery and plant development, we cloned a Passiflora morifolia putative homolog to a D-type cyclin, PmCYCD1, which showed high sequence similarity to other known plant D-type cyclins. We examined the expression patterns of PmCYCD1 during callus induction and growth in in vitro conditions. We observed incremented expression levels of PmCYCD1 correlated to increasing concentrations of sucrose, alpha-naphthalene acetic acid and 6-benzyladenine in the culture medium. Additionally, the results of in situ hybridization experiments indicated a dynamic spatial expression pattern for PmCYCD1 during callus growth.152233242Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

PmTCP1 encodes a putative TCP transcription factor and is differentially expressed during in vitro organogenesis in Passiflora

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)The genus Passiflora includes economically important passion fruits and over 600 other wild species. Micropropagation of Passiflora species is far from routine due to low regeneration frequencies and multiplication rates, so there is great interest in understanding the molecular control of the in vitro regeneration processes of these species. Here, we report the characterization of a Passiflora morifolia gene encoding PmTCP1, a putative TCP transcription factor that showed high sequence similarity to Arabidopsis class I TCPs. The expression patterns of the PmTCP1 gene during in vitro organogenesis and callus growth revealed differential expression of PmTCP1 modulated by different combinations of auxin and cytokinin concentrations in the culture medium. At a constant auxin concentration, cytokinin increased PmTCP1 expression, and at a constant cytokinin concentration, auxin repressed PmTCP1 expression. We also observed a correlation between PmTCP1 expression and the in vitro organogenesis of roots and shoots. We expect that these results will increase our understanding of the molecular networks and environmental signals that modulate the processes of organogenesis during plant development.5013644Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Avicultura Industrial: Novos Conceitos de Materiais, Concepções e Técnicas Construtivas Disponíveis para Galpões Avícolas Brasileiros Industrial Aviculture: New Concepts of Materials, Conceptions and Constructive Techniques Available for Brazilian Poultry Houses

Ao grande crescimento das fronteiras mercadológicas e ao extraordinário progresso científico verificado na avicultura industrial, contrapõe-se a pouca atenção que se deu, até recentemente, às técnicas de alojamento e, efetivamente, ao ambiente de criação das aves. Somente há questão de sete anos, com a perspectiva do processo de globalização que hoje movimenta a economia mundial, essa indústria passou a buscar, nas instalações e no ambiente, as possibilidades de melhoria no desempenho avícola e a redução dos custos de produção como forma de manter a competitividade. A produção de aves em alta densidade passou a ser imperativa e, dessa forma, a exigência de conforto térmico ambiental, que já havia crescido muito com o aumento da precocidade das aves, tornou-se ainda maior, como conseqüência da elevação da densidade de alojamento. Assim, para se manter competitiva, a indústria avícola brasileira está tendo de evoluir, rapidamente, de uma situação de quase indiferença aos princípios do acondicionamento térmico ambiente, para uma situação em que cada empresa ou integração deve tomar decisões relativas à adoção de concepções arquitetônicas e manejos inovadores, associados a sistemas de acondicionamento térmico naturais e artificiais, compatíveis com a sua realidade. Essas decisões incluem a readequação dos galpões já existentes e a concepção de novas unidades. Seguramente, estamos vivendo o momento de mais intensas transformações dos alojamentos avícolas brasileiros até então presenciado. Do exposto, esse trabalho busca abordar o planejamento, a concepção arquitetônica e os diferentes materiais e técnicas construtivas disponíveis para a indústria avícola brasileira no que diz respeito aos galpões de alojamentos das aves, visando propiciar um melhor acondicionamento térmico ambiente, compatível com as diferentes regiões climáticas brasileiras.<br>To the great growth of the market and the extraordinary scientific progress verified in the Brazilian poultry industry, little attention was given, until recently, to the lodging techniques and, indeed, to the environment of growing birds. This is only a seven year-old subject, and with the perspective of the globalization process that today moves the world economy, this industry started to look for facilities and environment, seeking the improvement possibilities in the poultry activity and the reduction of the production costs as a form of maintaining the competitiveness. The high-density production of birds became imperative and, this way, the demand of environmental thermal comfort, which had already grown a lot with the increase of the precocity of the birds, became even larger, as a consequence of the increase of lodging density. Thus, to continue being competitive, the Brazilian poultry industry has to develop, as soon as possible, from the situation of almost indifference of the beginning of the thermal atmosphere conditioning, to a situation in which each company or integration should take its own decisions of adoption of architectural conceptions and innovations associated with systems of thermal, natural and artificial conditioning, compatible with its reality. Decisions that already include the modifications of the existing houses and the conception of new units. We are witnessing, for sure, the moment of more intense transformations of the Brazilian poultry lodgings. From what was exposed, this work intends to approach the planning, the architectural conception and the different materials and available constructive techniques for the Brazilian poultry industry that refers to the lodgings of the birds, seeking to propitiate a better conditioned thermal atmosphere, compatible with the different Brazilian climatic areas