Analysis of perception of risk factors for public health in the use of hormones in assisted reproduction programs in cattle.

The purpose of this study was to analyze the knowledge and the risk perception of cattle farmers and veterinarians on the use of hormones used in assisted reproduction programs in cattle and to determine the possible occupational hazards.Proceedings of the 30th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Foz do Iguaçu, PR, Brazil, August 25th to 27th, 2016, and 32nd Meeting of the European Embryo Transfer Association (AETE); Barcelona, Spain, September 9th and 10th, 2016. A070. Título em português: Análise da percepção de fatores de risco para a saúde coletiva na utilização de hormônios em programas de reprodução assistida em bovinos

GnRH application in cows with low or no estrus expression evaluated with tail-chalk increases the pregnancy rate in beef cows submitted to TAI.

The objective was to evaluate the GnRH application in cows with low or poor estrus expression evaluated using tail chalk removal Score (ESCT) to increase TAI pregnancy rates.Proceedings of the 30th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Foz do Iguaçu, PR, Brazil, August 25th to 27th, 2016, and 32nd Meeting of the European Embryo Transfer Association (AETE); Barcelona, Spain, September 9th and 10th, 2016. A083. Título em português: Aplicação de GnRh em vacas com baixa ou sem expressão de estro avaliadas com bastões marcadores aumenta a taxa de prenhez em vacas de corte submetidas a IATF. Na publicação: Juliana Correa Borges

Effect of dietary supplementation of Nelore heifers with polyunsaturated fatty acids on cryotolerance and membrane lipid profile of fresh and vitrified in vitro-produced embryos.

The aim of this study was to evaluate the development and cryotolerance of IVP embryos from oocytes recovered from Nelore heifers (n = 16) fed with control diet (Control group) or fed with rumen-protected PUFA (Megalac-E®), for at least 60 days (Fat group).Proceedings of the 30th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Foz do Iguaçu, PR, Brazil, August 25th to 27th, 2016, and 32nd Meeting of the European Embryo Transfer Association (AETE); Barcelona, Spain, September 9th and 10th, 2016. A326. Support Biotechnologies: Cryopreservation and cryobiology, diagnosis through imaging, molecular biology and “omics”. Título em português: Influência da suplementação alimentar de novilhas Nelore com ácidos graxos poliinsaturados sobre a criotolerância e o perfil de lipídios de membrana de embriões produzidos in vitro frescos e vitrificados

Cryotolerance and pregnancy rates after exposure of bovine in vitro-produced embryos to forskolin and linoleic acid before vitrification.

The objective of the present study was to evaluate the effects of supplementation of in vitro culture (IVC) medium with drugs that stimulates the lipolysis (Forskolin: Forsk) and inhibit the lipogenesis (Linoleic Acid LA) on the intracytoplasmic lipid content and cryotolerance of bovine embryos (Experiment 1), as well as to evaluate the effect of treatment of embryos with Forsk on the pregnancy rates after transfer to synchronized recipients (Experiment 2).Proceedings of the 30th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Foz do Iguaçu, PR, Brazil, August 25th to 27th, 2016, and 32nd Meeting of the European Embryo Transfer Association (AETE); Barcelona, Spain, September 9th and 10th, 2016. A327 Support Biotechnologies: Cryopreservation and cryobiology, diagnosis through imaging, molecular biology and ?omics?. Título em português: Criotolerância e taxa de concepção após exposição de embriões bovinos produzidos in vitro ao Forskolin ou ácido linoleico antes da vitrificação

Membrane lipid profile monitored by mass spectrometry detected differences between fresh and vitrified in vitro-produced bovine embryos

This study aimed to evaluate the impact of vitrification on membrane lipid profile obtained by mass spectrometry (MS) of in vitro-produced bovine embryos. Matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) has been used to obtain individual embryo membrane lipid profiles. Due to conditions of analysis, mainly membrane lipids, most favorably phosphatidylcholines (PCs) and sphingomyelins (SMs) have been detected. The following ions described by their mass-to-charge ratio (m/z) and respective attribution presented increased relative abundance (1.2-20×) in the vitrified group: 703.5 [SM (16:0) + H]+; 722.5 [PC (40:3) + Na]+; 758.5 [PC (34:2) + H]+; 762.5 [PC (34:0) + H]+; 790.5 [PC (36:0) + H]+ and 810.5 [PC (38:4) + H]+ and/or [PC (36:1) + Na]+. The ion with a m/z 744.5 [PCp (34:1) and/or PCe (34:2)] was 3.4-fold more abundant in the fresh group. Interestingly, ions with m/z 722.5 or 744.5 indicate the presence of lipid species, which are more resistant to enzymatic degradation as they contain fatty acyl residues linked through ether type bonds (alkyl ether or plasmalogens, indicated by the lowercase 'e' and 'p', respectively) to the glycerol structure. The results indicate that cryopreservation impacts the membrane lipid profile, and that these alterations can be properly monitored by MALDI-MS. Membrane lipids can therefore be evaluated by MALDI-MS to monitor the effect of cryopreservation on membrane lipids, and to investigate changes in lipid profile that may reflect the metabolic response to the cryopreservation stress or changes in the environmental conditions.This study aimed to evaluate the impact of vitrification on membrane lipid profile obtained by mass spectrometry (MS) of in vitro-produced bovine embryos. 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