473 research outputs found
Use of a food frequency questionnaire in American Indian and Caucasian pregnant women: a validation study
BACKGROUND: Food frequency questionnaires (FFQs) have been validated in pregnant women, but few studies have focused specifically on low-income women and minorities. The purpose of this study was to examine the validity of the Harvard Service FFQ (HSFFQ) among low-income American Indian and Caucasian pregnant women. METHODS: The 100-item HSFFQ was administered three times to a sample of pregnant women, and two sets of 24-hour recalls (six total) were collected at approximately 12 and 28 weeks of gestation. The sample included a total of 283 pregnant women who completed Phase 1 of the study and 246 women who completed Phase 2 of the study. Deattenuated Pearson correlation coefficients were used to compare intakes of 24 nutrients estimated from the second and third FFQ to average intakes estimated from the week-12 and week-28 sets of diet recalls. RESULTS: Deattenuated correlations ranged from 0.09 (polyunsaturated fat) to 0.67 (calcium) for Phase 1 and from 0.27 (sucrose) to 0.63 (total fat) for Phase 2. Average deattenuated correlations for the two phases were 0.48 and 0.47, similar to those reported among other groups of pregnant women. CONCLUSION: The HSFFQ is a simple self-administered questionnaire that is useful in classifying low-income American Indian and Caucasian women according to relative dietary intake during pregnancy. Its use as a research tool in this population may provide important information about associations of nutrient intakes with pregnancy outcomes and may help to identify groups of women who would benefit most from nutritional interventions
Genetic diversity of Brazilian isolates of feline immunodeficiency virus
We isolated Feline immunodeficiency virus (FIV) from three adult domestic cats, originating from two open shelters in Brazil. Viruses were isolated from PBMC following co-cultivation with the feline T-lymphoblastoid cell line MYA-1. All amplified env gene products were cloned directly into pGL8MYA. The nucleic acid sequences of seven clones were determined and then compared with those of previously described isolates. The sequences of all of the Brazilian virus clones were distinct and phylogenetic analysis revealed that all belong to subtype B. Three variants isolated from one cat and two variants were isolated from each of the two other cats, indicating that intrahost diversity has the potential to pose problems for the treatment and diagnosis of FIV infection
Composite Fermion Metals from Dyon Black Holes and S-Duality
We propose that string theory in the background of dyon black holes in
four-dimensional anti-de Sitter spacetime is holographic dual to conformally
invariant composite Dirac fermion metal. By utilizing S-duality map, we show
that thermodynamic and transport properties of the black hole match with those
of composite fermion metal, exhibiting Fermi liquid-like. Built upon
Dirac-Schwinger-Zwanziger quantization condition, we argue that turning on
magnetic charges to electric black hole along the orbit of Gamma(2) subgroup of
SL(2,Z) is equivalent to attaching even unit of statistical flux quanta to
constituent fermions. Being at metallic point, the statistical magnetic flux is
interlocked to the background magnetic field. We find supporting evidences for
proposed holographic duality from study of internal energy of black hole and
probe bulk fermion motion in black hole background. They show good agreement
with ground-state energy of composite fermion metal in Thomas-Fermi
approximation and cyclotron motion of a constituent or composite fermion
excitation near Fermi-point.Comment: 30 pages, v2. 1 figure added, minor typos corrected; v3. revised
version to be published in JHE
The Quantized Hall Insulator: A New Insulator in Two-Dimensions
Quite generally, an insulator is theoretically defined by a vanishing
conductivity tensor at the absolute zero of temperature. In classical
insulators, such as band insulators, vanishing conductivities lead to diverging
resistivities. In other insulators, in particular when a high magnetic field
(B) is added, it is possible that while the magneto-resistance diverges, the
Hall resistance remains finite, which is known as a Hall insulator. In this
letter we demonstrate experimentally the existence of another, more exotic,
insulator. This insulator, which terminates the quantum Hall effect series in a
two-dimensional electron system, is characterized by a Hall resistance which is
approximately quantized in the quantum unit of resistance h/e^2. This insulator
is termed a quantized Hall insulator. In addition we show that for the same
sample, the insulating state preceding the QHE series, at low-B, is of the HI
kind.Comment: 4 page
Observation of the Fractional Quantum Hall Effect in Graphene
When electrons are confined in two dimensions and subjected to strong
magnetic fields, the Coulomb interactions between them become dominant and can
lead to novel states of matter such as fractional quantum Hall liquids. In
these liquids electrons linked to magnetic flux quanta form complex composite
quasipartices, which are manifested in the quantization of the Hall
conductivity as rational fractions of the conductance quantum. The recent
experimental discovery of an anomalous integer quantum Hall effect in graphene
has opened up a new avenue in the study of correlated 2D electronic systems, in
which the interacting electron wavefunctions are those of massless chiral
fermions. However, due to the prevailing disorder, graphene has thus far
exhibited only weak signatures of correlated electron phenomena, despite
concerted experimental efforts and intense theoretical interest. Here, we
report the observation of the fractional quantum Hall effect in ultraclean
suspended graphene, supporting the existence of strongly correlated electron
states in the presence of a magnetic field. In addition, at low carrier density
graphene becomes an insulator with an energy gap tunable by magnetic field.
These newly discovered quantum states offer the opportunity to study a new
state of matter of strongly correlated Dirac fermions in the presence of large
magnetic fields
Wigner Crystallization in a Quasi-3D Electronic System
When a strong magnetic field is applied perpendicularly (along z) to a sheet
confining electrons to two dimensions (x-y), highly correlated states emerge as
a result of the interplay between electron-electron interactions, confinement
and disorder. These so-called fractional quantum Hall (FQH) liquids form a
series of states which ultimately give way to a periodic electron solid that
crystallizes at high magnetic fields. This quantum phase of electrons has been
identified previously as a disorder-pinned two-dimensional Wigner crystal with
broken translational symmetry in the x-y plane. Here, we report our discovery
of a new insulating quantum phase of electrons when a very high magnetic field,
up to 45T, is applied in a geometry parallel (y-direction) to the
two-dimensional electron sheet. Our data point towards this new quantum phase
being an electron solid in a "quasi-3D" configuration induced by orbital
coupling with the parallel field
Unexpected features of branched flow through high-mobility two-dimensional electron gases
GaAs-based two-dimensional electron gases (2DEGs) show a wealth of remarkable
electronic states, and serve as the basis for fast transistors, research on
electrons in nanostructures, and prototypes of quantum-computing schemes. All
these uses depend on the extremely low levels of disorder in GaAs 2DEGs, with
low-temperature mean free paths ranging from microns to hundreds of microns.
Here we study how disorder affects the spatial structure of electron transport
by imaging electron flow in three different GaAs/AlGaAs 2DEGs, whose mobilities
range over an order of magnitude. As expected, electrons flow along narrow
branches that we find remain straight over a distance roughly proportional to
the mean free path. We also observe two unanticipated phenomena in
high-mobility samples. In our highest-mobility sample we observe an almost
complete absence of sharp impurity or defect scattering, indicated by the
complete suppression of quantum coherent interference fringes. Also, branched
flow through the chaotic potential of a high-mobility sample remains stable to
significant changes to the initial conditions of injected electrons.Comment: 22 pages, 4 figures, 1 tabl
Diet and body constitution in relation to subgroups of breast cancer defined by tumour grade, proliferation and key cell cycle regulators
BACKGROUND: The general lack of clear associations between diet and breast cancer in epidemiological studies may partly be explained by the fact that breast cancer is a heterogeneous disease that may have disparate genetic associations and different aetiological bases. METHOD: A total of 346 incident breast cancers in a prospective cohort of 17,035 women enrolled in the MalmΓΆ Diet and Cancer study (Sweden) were subcategorized according to conventional pathology parameters, proliferation and expression of key cell cycle regulators. Subcategories were compared with prediagnostic diet and body measurements using analysis of variance. RESULTS: A large hip circumference and high body mass index were associated with high grade tumours (P = 0.03 and 0.009, respectively), whereas low energy and unadjusted fat intakes were associated with high proliferation (P = 0.03 and 0.004, respectively). Low intakes of saturated, monounsaturated and polyunsaturated fatty acids were also associated with high proliferation (P = 0.02, 0.004 and 0.003, respectively). Low energy and unadjusted fat intakes were associated with cyclin D(1 )overexpression (P = 0.02 and 0.007, respectively), whereas cyclin E overexpression was positively correlated with fat intake. Oestrogen receptor status and expression of the tumour suppressor gene p27 were not associated with either diet or body constitution. CONCLUSION: Low energy and low total fat (polyunsaturated fatty acids in particular) intakes, and high body mass index were associated with relatively more malignant breast tumours. Dietary behaviours and body constitution may be associated with specific types of breast cancer defined by conventional pathology parameters and cyclin D(1 )and cyclin E expression. Further studies including healthy control individuals are needed to confirm our results
Identification of novel subgroup a variants with enhanced receptor binding and replicative capacity in primary isolates of anaemogenic strains of feline leukaemia virus
<b>BACKGROUND:</b>
The development of anaemia in feline leukaemia virus (FeLV)-infected cats is associated with the emergence of a novel viral subgroup, FeLV-C. FeLV-C arises from the subgroup that is transmitted, FeLV-A, through alterations in the amino acid sequence of the receptor binding domain (RBD) of the envelope glycoprotein that result in a shift in the receptor usage and the cell tropism of the virus. The factors that influence the transition from subgroup A to subgroup C remain unclear, one possibility is that a selective pressure in the host drives the acquisition of mutations in the RBD, creating A/C intermediates with enhanced abilities to interact with the FeLV-C receptor, FLVCR. In order to understand further the emergence of FeLV-C in the infected cat, we examined primary isolates of FeLV-C for evidence of FeLV-A variants that bore mutations consistent with a gradual evolution from FeLV-A to FeLV-C.<p></p>
<b>RESULTS:</b>
Within each isolate of FeLV-C, we identified variants that were ostensibly subgroup A by nucleic acid sequence comparisons, but which bore mutations in the RBD. One such mutation, N91D, was present in multiple isolates and when engineered into a molecular clone of the prototypic FeLV-A (Glasgow-1), enhanced replication was noted in feline cells. Expression of the N91D Env on murine leukaemia virus (MLV) pseudotypes enhanced viral entry mediated by the FeLV-A receptor THTR1 while soluble FeLV-A Env bearing the N91D mutation bound more efficiently to mouse or guinea pig cells bearing the FeLV-A and -C receptors. Long-term in vitro culture of variants bearing the N91D substitution in the presence of anti-FeLV gp70 antibodies did not result in the emergence of FeLV-C variants, suggesting that additional selective pressures in the infected cat may drive the subsequent evolution from subgroup A to subgroup C.<p></p>
<b>CONCLUSIONS:</b>
Our data support a model in which variants of FeLV-A, bearing subtle differences in the RBD of Env, may be predisposed towards enhanced replication in vivo and subsequent conversion to FeLV-C. The selection pressures in vivo that drive the emergence of FeLV-C in a proportion of infected cats remain to be established
Bone mineral density and the subsequent risk of cancer in the NHANES I follow-up cohort
BACKGROUD: Bone mineral density (BMD) is a marker of long-term estrogen exposure. BMD measurement has been used in this context to investigate the association of estrogen with breast cancer risk in three cohorts. In order to assess further BMD as a predictor of estrogen related cancer risk, the association of BMD with colorectal and corpus uteri cancer was investigated in the NHANES I Epidemiologic Followup Study (NHEFS) cohort along with breast cancer and prostate cancer. METHODS: Participants were members of the NHEFS cohort who had BMD measurement in 1974β1975. Age, race, and BMI adjusted rate ratios and 95% confidence intervals were calculated for incidence of cancers of the corpus uterus, breast, colorectum, prostate, and of osteoporosis and hip fracture related to baseline BMD. RESULTS: Data were available for 6046 individuals. One hundred cases of breast cancer, 94 prostate cancers, 115 colorectal cancers, 29 uterine cancers, 110 cases of hip fracture and 103 cases of osteoporosis were reported between 1974 and 1993. Hip fracture and osteoporosis were both significantly inversely associated with BMD. Uterine cancer was positively associated (p = 0.005, test for linear trend) and colorectal cancer negatively associated (p = 0.03) with BMD. No association was found between elevated BMD and incidence of breast cancer (p = 0.74) or prostate cancer (p = 0.37) in the overall cohort, although a weak association was seen between BMD and subsequent breast cancer incidence when BMD was measured in post-menopausal women (p = 0.04). CONCLUSION: The findings related to cancers of the uterus and colorectum as well as the weak association of BMD with breast cancer strengthen the use of BMD as a marker of estrogen exposure and cancer risk
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